Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6
A hybrid sensor kinase termed RetS (regulator of exopolysaccharide and Type III secretion) controls expression of numerous genes in Pseudomonas aeruginosa. To investigate the function of RetS in P. fluorescens FD6, the retS gene was disrupted. Genetic inactivation of retS resulted in enhanced produc...
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| Veröffentlicht in: | Microbiological research 2015-11, Vol.180, p.23-29 |
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| creator | Zhang, Qingxia Xiao, Qi Xu, Jingyou Tong, Yunhui Wen, Jia Chen, Xijun Wei, Lihui |
| description | A hybrid sensor kinase termed RetS (regulator of exopolysaccharide and Type III secretion) controls expression of numerous genes in Pseudomonas aeruginosa. To investigate the function of RetS in P. fluorescens FD6, the retS gene was disrupted. Genetic inactivation of retS resulted in enhanced production of 2, 4-diacetylphloroglucinol, pyrrolnitrin, and pyoluteorin. The retS mutant also exhibited significant increase in phlA-lacZ, prnA-lacZ, and pltA-lacZ transcription levels, influencing expression levels of the small regulatory RNAs RsmX and RsmZ. In the gacSretS double mutant, all the phenotypic changes caused by the retS deletion were reversed to the level of gacS single mutant. Furthermore, the retS mutation drastically elevated biofilm formation and improved the colonization ability of strain FD6 on wheat rhizospheres. Based on these results, we proposed that RetS negatively controlled the production of antibiotics through the Gac/Rsm pathway in P. fluorescens FD6. |
| doi_str_mv | 10.1016/j.micres.2015.07.005 |
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To investigate the function of RetS in P. fluorescens FD6, the retS gene was disrupted. Genetic inactivation of retS resulted in enhanced production of 2, 4-diacetylphloroglucinol, pyrrolnitrin, and pyoluteorin. The retS mutant also exhibited significant increase in phlA-lacZ, prnA-lacZ, and pltA-lacZ transcription levels, influencing expression levels of the small regulatory RNAs RsmX and RsmZ. In the gacSretS double mutant, all the phenotypic changes caused by the retS deletion were reversed to the level of gacS single mutant. Furthermore, the retS mutation drastically elevated biofilm formation and improved the colonization ability of strain FD6 on wheat rhizospheres. Based on these results, we proposed that RetS negatively controlled the production of antibiotics through the Gac/Rsm pathway in P. fluorescens FD6.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2015.07.005</identifier><identifier>PMID: 26505308</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Anti-Bacterial Agents - biosynthesis ; Antibiotics ; Bacterial Proteins - genetics ; Biofilms ; Gac/Rsm ; Galactosidases - metabolism ; Gene Silencing ; Meristem - microbiology ; Mutation ; Phenols - metabolism ; Phloroglucinol - analogs & derivatives ; Phloroglucinol - metabolism ; Pseudomonas ; Pseudomonas aeruginosa ; Pseudomonas fluorescens ; Pseudomonas fluorescens - genetics ; Pseudomonas fluorescens - metabolism ; Pyrroles - metabolism ; Pyrrolnitrin - metabolism ; RetS ; Rhizosphere ; Triticum - microbiology ; Triticum aestivum ; Virulence Factors - genetics</subject><ispartof>Microbiological research, 2015-11, Vol.180, p.23-29</ispartof><rights>2015 Elsevier GmbH</rights><rights>Copyright © 2015 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-527532de439e21c48570166b6c2fb98f9b18eb40d07397e72f2ccafc3115fc8a3</citedby><cites>FETCH-LOGICAL-c441t-527532de439e21c48570166b6c2fb98f9b18eb40d07397e72f2ccafc3115fc8a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944501315001317$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26505308$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Qingxia</creatorcontrib><creatorcontrib>Xiao, Qi</creatorcontrib><creatorcontrib>Xu, Jingyou</creatorcontrib><creatorcontrib>Tong, Yunhui</creatorcontrib><creatorcontrib>Wen, Jia</creatorcontrib><creatorcontrib>Chen, Xijun</creatorcontrib><creatorcontrib>Wei, Lihui</creatorcontrib><title>Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6</title><title>Microbiological research</title><addtitle>Microbiol Res</addtitle><description>A hybrid sensor kinase termed RetS (regulator of exopolysaccharide and Type III secretion) controls expression of numerous genes in Pseudomonas aeruginosa. To investigate the function of RetS in P. fluorescens FD6, the retS gene was disrupted. Genetic inactivation of retS resulted in enhanced production of 2, 4-diacetylphloroglucinol, pyrrolnitrin, and pyoluteorin. The retS mutant also exhibited significant increase in phlA-lacZ, prnA-lacZ, and pltA-lacZ transcription levels, influencing expression levels of the small regulatory RNAs RsmX and RsmZ. In the gacSretS double mutant, all the phenotypic changes caused by the retS deletion were reversed to the level of gacS single mutant. Furthermore, the retS mutation drastically elevated biofilm formation and improved the colonization ability of strain FD6 on wheat rhizospheres. Based on these results, we proposed that RetS negatively controlled the production of antibiotics through the Gac/Rsm pathway in P. fluorescens FD6.</description><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Antibiotics</subject><subject>Bacterial Proteins - genetics</subject><subject>Biofilms</subject><subject>Gac/Rsm</subject><subject>Galactosidases - metabolism</subject><subject>Gene Silencing</subject><subject>Meristem - microbiology</subject><subject>Mutation</subject><subject>Phenols - metabolism</subject><subject>Phloroglucinol - analogs & derivatives</subject><subject>Phloroglucinol - metabolism</subject><subject>Pseudomonas</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas fluorescens - metabolism</subject><subject>Pyrroles - metabolism</subject><subject>Pyrrolnitrin - metabolism</subject><subject>RetS</subject><subject>Rhizosphere</subject><subject>Triticum - microbiology</subject><subject>Triticum aestivum</subject><subject>Virulence Factors - genetics</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1r3DAQhkVJaLZJ_0EpOvZid_QtXwIlH21hoYUkZ2HLo6JlbSWSHei_j8ImPZacBoZnZt55CPnEoGXA9NddO0WfsbQcmGrBtADqHdkwzWwDmosjsoFOykYBEyfkQyk7ACY7y9-TE64VKAF2Q7ZXIaBfaAo043JD_-CMNM20n5c4xLREX-h9TuPql1jbcaa_C65jmtLcFxr2a6oRPM6FXl_qM3Ic-n3Bjy_1lNxdX91e_Gi2v77_vPi2bbyUbGkUN0rwEaXokDMvrTL1IT1oz8PQ2dANzOIgYQQjOoOGB-59H7xgTAVve3FKvhz21mQPK5bFTbGG2O_7GdNaHDNS284At29AueXKaiErKg-oz6mUjMHd5zj1-a9j4J6Vu507KHfPyh0YV5XXsc8vF9ZhwvHf0KvjCpwfAKxKHiNmV3zE2eMYc1XvxhT_f-EJvM6S-w</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Zhang, Qingxia</creator><creator>Xiao, Qi</creator><creator>Xu, Jingyou</creator><creator>Tong, Yunhui</creator><creator>Wen, Jia</creator><creator>Chen, Xijun</creator><creator>Wei, Lihui</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20151101</creationdate><title>Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6</title><author>Zhang, Qingxia ; Xiao, Qi ; Xu, Jingyou ; Tong, Yunhui ; Wen, Jia ; Chen, Xijun ; Wei, Lihui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-527532de439e21c48570166b6c2fb98f9b18eb40d07397e72f2ccafc3115fc8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Antibiotics</topic><topic>Bacterial Proteins - genetics</topic><topic>Biofilms</topic><topic>Gac/Rsm</topic><topic>Galactosidases - metabolism</topic><topic>Gene Silencing</topic><topic>Meristem - microbiology</topic><topic>Mutation</topic><topic>Phenols - metabolism</topic><topic>Phloroglucinol - analogs & derivatives</topic><topic>Phloroglucinol - metabolism</topic><topic>Pseudomonas</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas fluorescens - metabolism</topic><topic>Pyrroles - metabolism</topic><topic>Pyrrolnitrin - metabolism</topic><topic>RetS</topic><topic>Rhizosphere</topic><topic>Triticum - microbiology</topic><topic>Triticum aestivum</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Qingxia</creatorcontrib><creatorcontrib>Xiao, Qi</creatorcontrib><creatorcontrib>Xu, Jingyou</creatorcontrib><creatorcontrib>Tong, Yunhui</creatorcontrib><creatorcontrib>Wen, Jia</creatorcontrib><creatorcontrib>Chen, Xijun</creatorcontrib><creatorcontrib>Wei, Lihui</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Qingxia</au><au>Xiao, Qi</au><au>Xu, Jingyou</au><au>Tong, Yunhui</au><au>Wen, Jia</au><au>Chen, Xijun</au><au>Wei, Lihui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6</atitle><jtitle>Microbiological research</jtitle><addtitle>Microbiol Res</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>180</volume><spage>23</spage><epage>29</epage><pages>23-29</pages><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>A hybrid sensor kinase termed RetS (regulator of exopolysaccharide and Type III secretion) controls expression of numerous genes in Pseudomonas aeruginosa. To investigate the function of RetS in P. fluorescens FD6, the retS gene was disrupted. Genetic inactivation of retS resulted in enhanced production of 2, 4-diacetylphloroglucinol, pyrrolnitrin, and pyoluteorin. The retS mutant also exhibited significant increase in phlA-lacZ, prnA-lacZ, and pltA-lacZ transcription levels, influencing expression levels of the small regulatory RNAs RsmX and RsmZ. In the gacSretS double mutant, all the phenotypic changes caused by the retS deletion were reversed to the level of gacS single mutant. Furthermore, the retS mutation drastically elevated biofilm formation and improved the colonization ability of strain FD6 on wheat rhizospheres. Based on these results, we proposed that RetS negatively controlled the production of antibiotics through the Gac/Rsm pathway in P. fluorescens FD6.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>26505308</pmid><doi>10.1016/j.micres.2015.07.005</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Anti-Bacterial Agents - biosynthesis Antibiotics Bacterial Proteins - genetics Biofilms Gac/Rsm Galactosidases - metabolism Gene Silencing Meristem - microbiology Mutation Phenols - metabolism Phloroglucinol - analogs & derivatives Phloroglucinol - metabolism Pseudomonas Pseudomonas aeruginosa Pseudomonas fluorescens Pseudomonas fluorescens - genetics Pseudomonas fluorescens - metabolism Pyrroles - metabolism Pyrrolnitrin - metabolism RetS Rhizosphere Triticum - microbiology Triticum aestivum Virulence Factors - genetics |
| title | Effect of retS gene on antibiotics production in Pseudomonas fluorescens FD6 |
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