Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR
Mesenchymal stem cells (MSCs) have several features that make them an attractive option for potentiating cartilage repair. Synovium-derived (SMSCs) have been recently recognized as an excellent source. SRY-related HMG-box (Sox) family plays an important role in the proliferation and differentiation...
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| Veröffentlicht in: | Journal of molecular histology 2015-12, Vol.46 (6), p.467-473 |
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| creator | Zhang, Lei Chen, Shuo Bao, Nirong Yang, Chao Ti, Yunfan Zhou, Liwu Zhao, Jianning |
| description | Mesenchymal stem cells (MSCs) have several features that make them an attractive option for potentiating cartilage repair. Synovium-derived (SMSCs) have been recently recognized as an excellent source. SRY-related HMG-box (Sox) family plays an important role in the proliferation and differentiation of SMSCs. However, the role of Sox4 in human SMSCs remains elusive. In the present study, we investigated the role of Sox4 in SMSCs through gain-of-function studies and found that Sox4 promoted cell proliferation and chondrogenesis. Furthermore, Sox4 could directly bind to the promoter of long noncoding RNA DANCR and increased its expression. Finally, knockdown of DANCR could reverse the stimulative effect of Sox4 on the proliferation and chondrogenesis of SMSCs. Taken together, our data highlights the pivotal role of Sox4 in the proliferation and differentiation of SMSCs. |
| doi_str_mv | 10.1007/s10735-015-9638-z |
| format | Article |
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Synovium-derived (SMSCs) have been recently recognized as an excellent source. SRY-related HMG-box (Sox) family plays an important role in the proliferation and differentiation of SMSCs. However, the role of Sox4 in human SMSCs remains elusive. In the present study, we investigated the role of Sox4 in SMSCs through gain-of-function studies and found that Sox4 promoted cell proliferation and chondrogenesis. Furthermore, Sox4 could directly bind to the promoter of long noncoding RNA DANCR and increased its expression. Finally, knockdown of DANCR could reverse the stimulative effect of Sox4 on the proliferation and chondrogenesis of SMSCs. Taken together, our data highlights the pivotal role of Sox4 in the proliferation and differentiation of SMSCs.</description><identifier>ISSN: 1567-2379</identifier><identifier>EISSN: 1567-2387</identifier><identifier>DOI: 10.1007/s10735-015-9638-z</identifier><identifier>PMID: 26514989</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Differentiation - genetics ; Cell Proliferation ; Cells, Cultured ; Chondrogenesis - genetics ; Developmental Biology ; Gene Expression ; Gene Expression Regulation ; Humans ; Life Sciences ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Original Paper ; RNA Interference ; RNA, Long Noncoding - genetics ; SOXC Transcription Factors - genetics ; SOXC Transcription Factors - metabolism ; Stem Cells - cytology ; Stem Cells - metabolism ; Synovial Membrane - cytology ; Transcriptional Activation</subject><ispartof>Journal of molecular histology, 2015-12, Vol.46 (6), p.467-473</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-d21a3f4dae3c5fd08bdfe6722667ef277dc03c0dce7775408b009687810005913</citedby><cites>FETCH-LOGICAL-c475t-d21a3f4dae3c5fd08bdfe6722667ef277dc03c0dce7775408b009687810005913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10735-015-9638-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10735-015-9638-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26514989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Chen, Shuo</creatorcontrib><creatorcontrib>Bao, Nirong</creatorcontrib><creatorcontrib>Yang, Chao</creatorcontrib><creatorcontrib>Ti, Yunfan</creatorcontrib><creatorcontrib>Zhou, Liwu</creatorcontrib><creatorcontrib>Zhao, Jianning</creatorcontrib><title>Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR</title><title>Journal of molecular histology</title><addtitle>J Mol Hist</addtitle><addtitle>J Mol Histol</addtitle><description>Mesenchymal stem cells (MSCs) have several features that make them an attractive option for potentiating cartilage repair. Synovium-derived (SMSCs) have been recently recognized as an excellent source. SRY-related HMG-box (Sox) family plays an important role in the proliferation and differentiation of SMSCs. However, the role of Sox4 in human SMSCs remains elusive. In the present study, we investigated the role of Sox4 in SMSCs through gain-of-function studies and found that Sox4 promoted cell proliferation and chondrogenesis. Furthermore, Sox4 could directly bind to the promoter of long noncoding RNA DANCR and increased its expression. Finally, knockdown of DANCR could reverse the stimulative effect of Sox4 on the proliferation and chondrogenesis of SMSCs. Taken together, our data highlights the pivotal role of Sox4 in the proliferation and differentiation of SMSCs.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Chondrogenesis - genetics</subject><subject>Developmental Biology</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Original Paper</subject><subject>RNA Interference</subject><subject>RNA, Long Noncoding - genetics</subject><subject>SOXC Transcription Factors - genetics</subject><subject>SOXC Transcription Factors - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Synovial Membrane - cytology</subject><subject>Transcriptional Activation</subject><issn>1567-2379</issn><issn>1567-2387</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkctuFDEQRS1ERELgA9ggS2zYNPjR7cdyNDylKJESWFuOXT3jqNsOdveI5Bf4aTzqMEJIKCuXqs6tctVF6BUl7ygh8n2hRPKuIbRrtOCquX-CTmgnZMO4kk8PsdTH6HkpN4QwJVr9DB0z0dFWK32Cfl2lny2GuLXRQcFum6LPaQMxOOxD30OGOAU7hRSxjR7f5jSEml0yqcfbebQRl7uYdmEeGw857MDjMsGIHQwD3gWLrZvC7iAZUtzgmKJLPtTo8nyFP6zO15cv0FFvhwIvH95T9P3Tx2_rL83Zxeev69VZ41rZTY1n1PK-9Ra463pP1LXvQUjGhJDQMym9I9wR70BK2bW1TogWSqp6M9Jpyk_R26VvXebHDGUyYyj7v9oIaS6GylYozXS96eMop1JxTbuKvvkHvUlzjnWRPVUHt7LVlaIL5XIqJUNvbnMYbb4zlJi9qWYx1VRTzd5Uc181rx86z9cj-IPij4sVYAtQailuIP81-r9dfwOafK35</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Zhang, Lei</creator><creator>Chen, Shuo</creator><creator>Bao, Nirong</creator><creator>Yang, Chao</creator><creator>Ti, Yunfan</creator><creator>Zhou, Liwu</creator><creator>Zhao, Jianning</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20151201</creationdate><title>Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR</title><author>Zhang, Lei ; Chen, Shuo ; Bao, Nirong ; Yang, Chao ; Ti, Yunfan ; Zhou, Liwu ; Zhao, Jianning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-d21a3f4dae3c5fd08bdfe6722667ef277dc03c0dce7775408b009687810005913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Chondrogenesis - genetics</topic><topic>Developmental Biology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Original Paper</topic><topic>RNA Interference</topic><topic>RNA, Long Noncoding - genetics</topic><topic>SOXC Transcription Factors - genetics</topic><topic>SOXC Transcription Factors - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Synovial Membrane - cytology</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Chen, Shuo</creatorcontrib><creatorcontrib>Bao, Nirong</creatorcontrib><creatorcontrib>Yang, Chao</creatorcontrib><creatorcontrib>Ti, Yunfan</creatorcontrib><creatorcontrib>Zhou, Liwu</creatorcontrib><creatorcontrib>Zhao, Jianning</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Journal of molecular histology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Lei</au><au>Chen, Shuo</au><au>Bao, Nirong</au><au>Yang, Chao</au><au>Ti, Yunfan</au><au>Zhou, Liwu</au><au>Zhao, Jianning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR</atitle><jtitle>Journal of molecular histology</jtitle><stitle>J Mol Hist</stitle><addtitle>J Mol Histol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>46</volume><issue>6</issue><spage>467</spage><epage>473</epage><pages>467-473</pages><issn>1567-2379</issn><eissn>1567-2387</eissn><abstract>Mesenchymal stem cells (MSCs) have several features that make them an attractive option for potentiating cartilage repair. Synovium-derived (SMSCs) have been recently recognized as an excellent source. SRY-related HMG-box (Sox) family plays an important role in the proliferation and differentiation of SMSCs. However, the role of Sox4 in human SMSCs remains elusive. In the present study, we investigated the role of Sox4 in SMSCs through gain-of-function studies and found that Sox4 promoted cell proliferation and chondrogenesis. Furthermore, Sox4 could directly bind to the promoter of long noncoding RNA DANCR and increased its expression. Finally, knockdown of DANCR could reverse the stimulative effect of Sox4 on the proliferation and chondrogenesis of SMSCs. Taken together, our data highlights the pivotal role of Sox4 in the proliferation and differentiation of SMSCs.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26514989</pmid><doi>10.1007/s10735-015-9638-z</doi><tpages>7</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cell Biology Cell Differentiation - genetics Cell Proliferation Cells, Cultured Chondrogenesis - genetics Developmental Biology Gene Expression Gene Expression Regulation Humans Life Sciences Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Original Paper RNA Interference RNA, Long Noncoding - genetics SOXC Transcription Factors - genetics SOXC Transcription Factors - metabolism Stem Cells - cytology Stem Cells - metabolism Synovial Membrane - cytology Transcriptional Activation |
| title | Sox4 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cell via activation of long noncoding RNA DANCR |
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