Evolving to the optoelectronic mouse for phycotoxin analysis in shellfish
Despite ethical and technical concerns, the in vivo method, or more commonly referred to mouse bioassay (MBA), is employed globally as a reference method for phycotoxin analysis in shellfish. This is particularly the case for paralytic shellfish poisoning (PSP) and emerging toxin monitoring. A high-...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2014-11, Vol.406 (27), p.6867-6881 |
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| creator | Campbell, Katrina McNamee, Sara E. Huet, Anne-Catherine Delahaut, Philippe Vilarino, Natalia Botana, Luis M. Poli, Mark Elliott, Christopher T. |
| description | Despite ethical and technical concerns, the in vivo method, or more commonly referred to mouse bioassay (MBA), is employed globally as a reference method for phycotoxin analysis in shellfish. This is particularly the case for paralytic shellfish poisoning (PSP) and emerging toxin monitoring. A high-performance liquid chromatography method (HPLC-FLD) has been developed for PSP toxin analysis, but due to difficulties and limitations in the method, this procedure has not been fully implemented as a replacement. Detection of the diarrhetic shellfish poisoning (DSP) toxins has moved towards LC-mass spectrometry (MS) analysis, whereas the analysis of the amnesic shellfish poisoning (ASP) toxin domoic acid is performed by HPLC. Although alternative methods of detection to the MBA have been described, each procedure is specific for a particular toxin and its analogues, with each group of toxins requiring separate analysis utilising different extraction procedures and analytical equipment. In addition, consideration towards the detection of unregulated and emerging toxins on the replacement of the MBA must be given. The ideal scenario for the monitoring of phycotoxins in shellfish and seafood would be to evolve to multiple toxin detection on a single bioanalytical sensing platform, i.e. ‘an artificial mouse’. Immunologically based techniques and in particular surface plasmon resonance technology have been shown as a highly promising bioanalytical tool offering rapid, real-time detection requiring minimal quantities of toxin standards. A Biacore Q and a prototype multiplex SPR biosensor have been evaluated for their ability to be fit for purpose for the simultaneous detection of key regulated phycotoxin groups and the emerging toxin palytoxin. Deemed more applicable due to the separate flow channels, the prototype performance for domoic acid, okadaic acid, saxitoxin, and palytoxin calibration curves in shellfish achieved detection limits (IC
20
) of 4,000, 36, 144 and 46 μg/kg of mussel, respectively. A one-step extraction procedure demonstrated recoveries greater than 80 % for all toxins. For validation of the method at the 95 % confidence limit, the decision limits (CCα) determined from an extracted matrix curve were calculated to be 450, 36 and 24 μg/kg, and the detection capability (CCβ) as a screening method is ≤10 mg/kg, ≤160 μg/kg and ≤400 μg/kg for domoic acid, okadaic acid and saxitoxin, respectively. |
| doi_str_mv | 10.1007/s00216-014-8156-2 |
| format | Article |
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20
) of 4,000, 36, 144 and 46 μg/kg of mussel, respectively. A one-step extraction procedure demonstrated recoveries greater than 80 % for all toxins. For validation of the method at the 95 % confidence limit, the decision limits (CCα) determined from an extracted matrix curve were calculated to be 450, 36 and 24 μg/kg, and the detection capability (CCβ) as a screening method is ≤10 mg/kg, ≤160 μg/kg and ≤400 μg/kg for domoic acid, okadaic acid and saxitoxin, respectively.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-014-8156-2</identifier><identifier>PMID: 25245418</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Advanced Food Analysis ; Amnesic shellfish poisoning ; Analysis ; Analytical Chemistry ; Animals ; Bioassays ; Biochemistry ; Biosensing Techniques ; Biosensors ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chromatography, High Pressure Liquid ; Confidence limits ; Detection limits ; Diarrhetic shellfish poisoning ; Domoic acid ; Ethics ; Extraction procedures ; Flow channels ; Food Science ; High performance liquid chromatography ; In vivo methods and tests ; Laboratory Medicine ; Limit of Detection ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Mathematical analysis ; Monitoring ; Monitoring/Environmental Analysis ; Okadaic acid ; Optoelectronics ; Palytoxin ; Paralytic shellfish poisoning ; Phycotoxins ; Poisoning ; Prototypes ; Reproducibility of Results ; Research Paper ; Saxitoxin ; Seafood ; Shellfish ; Surface Plasmon Resonance ; Surgical implants ; Toxins ; Toxins, Biological - analysis</subject><ispartof>Analytical and bioanalytical chemistry, 2014-11, Vol.406 (27), p.6867-6881</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2014.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c584t-f1e409370e9a117ec2520f8e34c69c519a9797f390a8541a09818b61272557863</citedby><cites>FETCH-LOGICAL-c584t-f1e409370e9a117ec2520f8e34c69c519a9797f390a8541a09818b61272557863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-014-8156-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-014-8156-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25245418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Campbell, Katrina</creatorcontrib><creatorcontrib>McNamee, Sara E.</creatorcontrib><creatorcontrib>Huet, Anne-Catherine</creatorcontrib><creatorcontrib>Delahaut, Philippe</creatorcontrib><creatorcontrib>Vilarino, Natalia</creatorcontrib><creatorcontrib>Botana, Luis M.</creatorcontrib><creatorcontrib>Poli, Mark</creatorcontrib><creatorcontrib>Elliott, Christopher T.</creatorcontrib><title>Evolving to the optoelectronic mouse for phycotoxin analysis in shellfish</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Despite ethical and technical concerns, the in vivo method, or more commonly referred to mouse bioassay (MBA), is employed globally as a reference method for phycotoxin analysis in shellfish. This is particularly the case for paralytic shellfish poisoning (PSP) and emerging toxin monitoring. A high-performance liquid chromatography method (HPLC-FLD) has been developed for PSP toxin analysis, but due to difficulties and limitations in the method, this procedure has not been fully implemented as a replacement. Detection of the diarrhetic shellfish poisoning (DSP) toxins has moved towards LC-mass spectrometry (MS) analysis, whereas the analysis of the amnesic shellfish poisoning (ASP) toxin domoic acid is performed by HPLC. Although alternative methods of detection to the MBA have been described, each procedure is specific for a particular toxin and its analogues, with each group of toxins requiring separate analysis utilising different extraction procedures and analytical equipment. In addition, consideration towards the detection of unregulated and emerging toxins on the replacement of the MBA must be given. The ideal scenario for the monitoring of phycotoxins in shellfish and seafood would be to evolve to multiple toxin detection on a single bioanalytical sensing platform, i.e. ‘an artificial mouse’. Immunologically based techniques and in particular surface plasmon resonance technology have been shown as a highly promising bioanalytical tool offering rapid, real-time detection requiring minimal quantities of toxin standards. A Biacore Q and a prototype multiplex SPR biosensor have been evaluated for their ability to be fit for purpose for the simultaneous detection of key regulated phycotoxin groups and the emerging toxin palytoxin. Deemed more applicable due to the separate flow channels, the prototype performance for domoic acid, okadaic acid, saxitoxin, and palytoxin calibration curves in shellfish achieved detection limits (IC
20
) of 4,000, 36, 144 and 46 μg/kg of mussel, respectively. A one-step extraction procedure demonstrated recoveries greater than 80 % for all toxins. For validation of the method at the 95 % confidence limit, the decision limits (CCα) determined from an extracted matrix curve were calculated to be 450, 36 and 24 μg/kg, and the detection capability (CCβ) as a screening method is ≤10 mg/kg, ≤160 μg/kg and ≤400 μg/kg for domoic acid, okadaic acid and saxitoxin, respectively.</description><subject>Acids</subject><subject>Advanced Food Analysis</subject><subject>Amnesic shellfish poisoning</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Bioassays</subject><subject>Biochemistry</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Confidence limits</subject><subject>Detection limits</subject><subject>Diarrhetic shellfish poisoning</subject><subject>Domoic acid</subject><subject>Ethics</subject><subject>Extraction procedures</subject><subject>Flow channels</subject><subject>Food Science</subject><subject>High performance liquid chromatography</subject><subject>In vivo methods and tests</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mathematical analysis</subject><subject>Monitoring</subject><subject>Monitoring/Environmental Analysis</subject><subject>Okadaic acid</subject><subject>Optoelectronics</subject><subject>Palytoxin</subject><subject>Paralytic shellfish poisoning</subject><subject>Phycotoxins</subject><subject>Poisoning</subject><subject>Prototypes</subject><subject>Reproducibility of Results</subject><subject>Research Paper</subject><subject>Saxitoxin</subject><subject>Seafood</subject><subject>Shellfish</subject><subject>Surface Plasmon Resonance</subject><subject>Surgical implants</subject><subject>Toxins</subject><subject>Toxins, Biological - analysis</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU9rFTEUxYMotlY_gBsZcONm6r2Z_F2WUrVQcKPrkKY376XMTJ7JvOL79uYxtYIgkkUuye-ee5LD2FuEcwTQHysAR9UDit6gVD1_xk5Roem5kvD8qRb8hL2q9R4ApUH1kp1wyYUUaE7Z9dVDHh_SvOmW3C1b6vJuyTRSWEqeU-imvK_UxVy63fYQ8pJ_prnzsx8PNdWu1XVL4xhT3b5mL6IfK7153M_Y909X3y6_9DdfP19fXtz0QRqx9BFJgB00kPWImkLzAtHQIIKyQaL1VlsdBwveNIserEFzq5BrLqU2ajhjH1bdXck_9lQXN6Uamgk_UzPrUAtlLEjE_6NK4mDAat7Q93-h93lf2jur402q_SdXR-p8pTZ-JJfmmJfiQ1t3NKWQZ4qpnV8MxigzCCFbA64NoeRaC0W3K2ny5eAQ3DFDt2boWobumKE7Dnn3aGV_O9HdU8fv0BrAV6C2q3lD5Y_Xf6v-Aqg_o6U</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Campbell, Katrina</creator><creator>McNamee, Sara 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to the optoelectronic mouse for phycotoxin analysis in shellfish</title><author>Campbell, Katrina ; McNamee, Sara E. ; Huet, Anne-Catherine ; Delahaut, Philippe ; Vilarino, Natalia ; Botana, Luis M. ; Poli, Mark ; Elliott, Christopher T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c584t-f1e409370e9a117ec2520f8e34c69c519a9797f390a8541a09818b61272557863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acids</topic><topic>Advanced Food Analysis</topic><topic>Amnesic shellfish poisoning</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Bioassays</topic><topic>Biochemistry</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Confidence limits</topic><topic>Detection limits</topic><topic>Diarrhetic shellfish poisoning</topic><topic>Domoic acid</topic><topic>Ethics</topic><topic>Extraction procedures</topic><topic>Flow channels</topic><topic>Food Science</topic><topic>High performance liquid chromatography</topic><topic>In vivo methods and tests</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Mathematical analysis</topic><topic>Monitoring</topic><topic>Monitoring/Environmental Analysis</topic><topic>Okadaic acid</topic><topic>Optoelectronics</topic><topic>Palytoxin</topic><topic>Paralytic shellfish poisoning</topic><topic>Phycotoxins</topic><topic>Poisoning</topic><topic>Prototypes</topic><topic>Reproducibility of Results</topic><topic>Research Paper</topic><topic>Saxitoxin</topic><topic>Seafood</topic><topic>Shellfish</topic><topic>Surface Plasmon 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chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>406</volume><issue>27</issue><spage>6867</spage><epage>6881</epage><pages>6867-6881</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Despite ethical and technical concerns, the in vivo method, or more commonly referred to mouse bioassay (MBA), is employed globally as a reference method for phycotoxin analysis in shellfish. This is particularly the case for paralytic shellfish poisoning (PSP) and emerging toxin monitoring. A high-performance liquid chromatography method (HPLC-FLD) has been developed for PSP toxin analysis, but due to difficulties and limitations in the method, this procedure has not been fully implemented as a replacement. Detection of the diarrhetic shellfish poisoning (DSP) toxins has moved towards LC-mass spectrometry (MS) analysis, whereas the analysis of the amnesic shellfish poisoning (ASP) toxin domoic acid is performed by HPLC. Although alternative methods of detection to the MBA have been described, each procedure is specific for a particular toxin and its analogues, with each group of toxins requiring separate analysis utilising different extraction procedures and analytical equipment. In addition, consideration towards the detection of unregulated and emerging toxins on the replacement of the MBA must be given. The ideal scenario for the monitoring of phycotoxins in shellfish and seafood would be to evolve to multiple toxin detection on a single bioanalytical sensing platform, i.e. ‘an artificial mouse’. Immunologically based techniques and in particular surface plasmon resonance technology have been shown as a highly promising bioanalytical tool offering rapid, real-time detection requiring minimal quantities of toxin standards. A Biacore Q and a prototype multiplex SPR biosensor have been evaluated for their ability to be fit for purpose for the simultaneous detection of key regulated phycotoxin groups and the emerging toxin palytoxin. Deemed more applicable due to the separate flow channels, the prototype performance for domoic acid, okadaic acid, saxitoxin, and palytoxin calibration curves in shellfish achieved detection limits (IC
20
) of 4,000, 36, 144 and 46 μg/kg of mussel, respectively. A one-step extraction procedure demonstrated recoveries greater than 80 % for all toxins. For validation of the method at the 95 % confidence limit, the decision limits (CCα) determined from an extracted matrix curve were calculated to be 450, 36 and 24 μg/kg, and the detection capability (CCβ) as a screening method is ≤10 mg/kg, ≤160 μg/kg and ≤400 μg/kg for domoic acid, okadaic acid and saxitoxin, respectively.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25245418</pmid><doi>10.1007/s00216-014-8156-2</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2014-11, Vol.406 (27), p.6867-6881 |
| issn | 1618-2642 1618-2650 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Acids Advanced Food Analysis Amnesic shellfish poisoning Analysis Analytical Chemistry Animals Bioassays Biochemistry Biosensing Techniques Biosensors Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Chromatography, High Pressure Liquid Confidence limits Detection limits Diarrhetic shellfish poisoning Domoic acid Ethics Extraction procedures Flow channels Food Science High performance liquid chromatography In vivo methods and tests Laboratory Medicine Limit of Detection Liquid chromatography Mass spectrometry Mass spectroscopy Mathematical analysis Monitoring Monitoring/Environmental Analysis Okadaic acid Optoelectronics Palytoxin Paralytic shellfish poisoning Phycotoxins Poisoning Prototypes Reproducibility of Results Research Paper Saxitoxin Seafood Shellfish Surface Plasmon Resonance Surgical implants Toxins Toxins, Biological - analysis |
| title | Evolving to the optoelectronic mouse for phycotoxin analysis in shellfish |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T18%3A41%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evolving%20to%20the%20optoelectronic%20mouse%20for%20phycotoxin%20analysis%20in%20shellfish&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Campbell,%20Katrina&rft.date=2014-11-01&rft.volume=406&rft.issue=27&rft.spage=6867&rft.epage=6881&rft.pages=6867-6881&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-014-8156-2&rft_dat=%3Cgale_proqu%3EA388683445%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2511650262&rft_id=info:pmid/25245418&rft_galeid=A388683445&rfr_iscdi=true |