Integrated plasma and urine metabolomics coupled with HPLC/QTOF-MS and chemometric analysis on potential biomarkers in liver injury and hepatoprotective effects of Er-Zhi-Wan
Metabolomics techniques are the comprehensive assessment of endogenous metabolites in a biological system and may provide additional insight into the molecular mechanisms. Er-Zhi-Wan (EZW) is a traditional Chinese medicine formula, which contains Fructus Ligustri Lucidi (FLL) and Herba Ecliptae (HE)...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2014-11, Vol.406 (28), p.7367-7378 |
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| creator | Yao, Weifeng Gu, Haiwei Zhu, Jiangjiang Barding, Gregory Cheng, Haibo Bao, Beihua Zhang, Li Ding, Anwei Li, Wei |
| description | Metabolomics techniques are the comprehensive assessment of endogenous metabolites in a biological system and may provide additional insight into the molecular mechanisms. Er-Zhi-Wan (EZW) is a traditional Chinese medicine formula, which contains
Fructus Ligustri Lucidi
(FLL) and
Herba Ecliptae
(HE). EZW is widely used to prevent and treat various liver injuries through the nourishment of the liver. However, the precise molecular mechanism of hepatoprotective effects has not been comprehensively explored. Here, an integrated metabolomics strategy was designed to assess the effects and possible mechanisms of EZW against carbon tetrachloride-induced liver injury, a commonly used model of both acute and chronic liver intoxication. High-performance chromatography/quadrupole time-of-flight mass spectrometry (HPLC/QTOF-MS) combined with chemometric approaches including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to discover differentiating metabolites in metabolomics data of rat plasma and urine. Results indicate six differentiating metabolites, tryptophan, sphinganine, tetrahydrocorticosterone, pipecolic acid,
l
-2-amino-3-oxobutanoic acid and phosphoribosyl pyrophosphate, in the positive mode. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan metabolism, sphingolipid metabolism, steroid hormone biosynthesis, lysine degradation, glycine, serine and threonine metabolism, and pentose phosphate pathway. Of note, EZW has a potential pharmacological effect, which might be through regulating multiple perturbed pathways to the normal state. Our findings also showed that the robust integrated metabolomics techniques are promising for identifying more biomarkers and pathways and helping to clarify the function mechanisms of traditional Chinese medicine.
Graphical Abstract
Overview of the integrated metabolomics strategy |
| doi_str_mv | 10.1007/s00216-014-8169-x |
| format | Article |
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Fructus Ligustri Lucidi
(FLL) and
Herba Ecliptae
(HE). EZW is widely used to prevent and treat various liver injuries through the nourishment of the liver. However, the precise molecular mechanism of hepatoprotective effects has not been comprehensively explored. Here, an integrated metabolomics strategy was designed to assess the effects and possible mechanisms of EZW against carbon tetrachloride-induced liver injury, a commonly used model of both acute and chronic liver intoxication. High-performance chromatography/quadrupole time-of-flight mass spectrometry (HPLC/QTOF-MS) combined with chemometric approaches including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to discover differentiating metabolites in metabolomics data of rat plasma and urine. Results indicate six differentiating metabolites, tryptophan, sphinganine, tetrahydrocorticosterone, pipecolic acid,
l
-2-amino-3-oxobutanoic acid and phosphoribosyl pyrophosphate, in the positive mode. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan metabolism, sphingolipid metabolism, steroid hormone biosynthesis, lysine degradation, glycine, serine and threonine metabolism, and pentose phosphate pathway. Of note, EZW has a potential pharmacological effect, which might be through regulating multiple perturbed pathways to the normal state. Our findings also showed that the robust integrated metabolomics techniques are promising for identifying more biomarkers and pathways and helping to clarify the function mechanisms of traditional Chinese medicine.
Graphical Abstract
Overview of the integrated metabolomics strategy</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-014-8169-x</identifier><identifier>PMID: 25245419</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Animals ; Biochemistry ; Biological markers ; Biomarkers ; Biomarkers - blood ; Biomarkers - urine ; Biosynthesis ; Carbon tetrachloride ; Carbon Tetrachloride - toxicity ; Characterization and Evaluation of Materials ; Chemical and Drug Induced Liver Injury - blood ; Chemical and Drug Induced Liver Injury - diagnosis ; Chemical and Drug Induced Liver Injury - prevention & control ; Chemical and Drug Induced Liver Injury - urine ; Chemistry ; Chemistry and Materials Science ; Chemometrics ; Chinese medicine ; Chromatography, High Pressure Liquid ; Discriminant Analysis ; Food Science ; Glycine ; Herbal medicine ; High performance liquid chromatography ; Injuries ; Injury analysis ; Injury prevention ; Intoxication ; Laboratory Medicine ; Lipid metabolism ; Liquid chromatography ; Liver ; Liver - drug effects ; Liver - metabolism ; Lysine ; Male ; Mass spectrometry ; Mass spectroscopy ; Mathematical models ; Medicine, Chinese Traditional ; Metabolism ; Metabolites ; Metabolomics ; Metabolomics - methods ; Molecular modelling ; Monitoring/Environmental Analysis ; Pathways ; Pentose ; Pentose phosphate pathway ; Phosphoribosyl pyrophosphate ; Principal components analysis ; Quadrupoles ; Rats ; Rats, Wistar ; Research Paper ; Serine ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Sphinganine ; Steroids ; Threonine ; Traditional Chinese medicine ; Tryptophan</subject><ispartof>Analytical and bioanalytical chemistry, 2014-11, Vol.406 (28), p.7367-7378</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2014.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c575t-f58451ab6d055ecb3f70877254ba3c9ab6cf072c941029ac8da85e278e310f53</citedby><cites>FETCH-LOGICAL-c575t-f58451ab6d055ecb3f70877254ba3c9ab6cf072c941029ac8da85e278e310f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-014-8169-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-014-8169-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25245419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yao, Weifeng</creatorcontrib><creatorcontrib>Gu, Haiwei</creatorcontrib><creatorcontrib>Zhu, Jiangjiang</creatorcontrib><creatorcontrib>Barding, Gregory</creatorcontrib><creatorcontrib>Cheng, Haibo</creatorcontrib><creatorcontrib>Bao, Beihua</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Ding, Anwei</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><title>Integrated plasma and urine metabolomics coupled with HPLC/QTOF-MS and chemometric analysis on potential biomarkers in liver injury and hepatoprotective effects of Er-Zhi-Wan</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Metabolomics techniques are the comprehensive assessment of endogenous metabolites in a biological system and may provide additional insight into the molecular mechanisms. Er-Zhi-Wan (EZW) is a traditional Chinese medicine formula, which contains
Fructus Ligustri Lucidi
(FLL) and
Herba Ecliptae
(HE). EZW is widely used to prevent and treat various liver injuries through the nourishment of the liver. However, the precise molecular mechanism of hepatoprotective effects has not been comprehensively explored. Here, an integrated metabolomics strategy was designed to assess the effects and possible mechanisms of EZW against carbon tetrachloride-induced liver injury, a commonly used model of both acute and chronic liver intoxication. High-performance chromatography/quadrupole time-of-flight mass spectrometry (HPLC/QTOF-MS) combined with chemometric approaches including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to discover differentiating metabolites in metabolomics data of rat plasma and urine. Results indicate six differentiating metabolites, tryptophan, sphinganine, tetrahydrocorticosterone, pipecolic acid,
l
-2-amino-3-oxobutanoic acid and phosphoribosyl pyrophosphate, in the positive mode. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan metabolism, sphingolipid metabolism, steroid hormone biosynthesis, lysine degradation, glycine, serine and threonine metabolism, and pentose phosphate pathway. Of note, EZW has a potential pharmacological effect, which might be through regulating multiple perturbed pathways to the normal state. Our findings also showed that the robust integrated metabolomics techniques are promising for identifying more biomarkers and pathways and helping to clarify the function mechanisms of traditional Chinese medicine.
Graphical Abstract
Overview of the integrated metabolomics strategy</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological markers</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - urine</subject><subject>Biosynthesis</subject><subject>Carbon tetrachloride</subject><subject>Carbon Tetrachloride - toxicity</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical and Drug Induced Liver Injury - blood</subject><subject>Chemical and Drug Induced Liver Injury - diagnosis</subject><subject>Chemical and Drug Induced Liver Injury - prevention & control</subject><subject>Chemical and Drug Induced Liver Injury - urine</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemometrics</subject><subject>Chinese medicine</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Discriminant Analysis</subject><subject>Food Science</subject><subject>Glycine</subject><subject>Herbal medicine</subject><subject>High performance liquid chromatography</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Injury prevention</subject><subject>Intoxication</subject><subject>Laboratory Medicine</subject><subject>Lipid metabolism</subject><subject>Liquid chromatography</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Lysine</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mathematical models</subject><subject>Medicine, Chinese Traditional</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Metabolomics - methods</subject><subject>Molecular modelling</subject><subject>Monitoring/Environmental Analysis</subject><subject>Pathways</subject><subject>Pentose</subject><subject>Pentose phosphate pathway</subject><subject>Phosphoribosyl pyrophosphate</subject><subject>Principal components analysis</subject><subject>Quadrupoles</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Research Paper</subject><subject>Serine</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Sphinganine</subject><subject>Steroids</subject><subject>Threonine</subject><subject>Traditional Chinese medicine</subject><subject>Tryptophan</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNksFu1DAQhiMEoqXwAFyQJS69pLWdOE6O1aqllRYVxEpIXCzHmex6SezFdqD7Ujwjs93SCgSC5JBJ5vt_TTx_lr1k9IRRKk8jpZxVOWVlXrOqyW8eZYesYnXOK0Ef39clP8iexbimlAnknmYHXPBSlKw5zL5fuQTLoBN0ZDPoOGqiXUemYB2QEZJu_eBHayIxftoMSH2zaUUu381np-8X1xf52w-3ArOC0SMfrMF3PWyjjcQ7svEJXLJ6IK31ow6fIURiHRnsVwhYrKewvTVYwUYnvwnIm4RNAn2PFZr05Dzkn1Y2_6jd8-xJr4cIL-6eR9ni4nwxu8zn12-uZmfz3AgpUt6LuhRMt1VHhQDTFr2ktZRclK0uTIMN01PJTVMyyhtt6k7XArisoWC0F8VRdry3xXm-TBCTGm00MAzagZ-iYrKs6gZPv_w3WglW1Fww-R8oq0qJF0P09W_o2k8BzzUqtGKikkLyB2qpB1DW9T4FbXam6qyomwp3LHYTnvyBwrsDXKx30Fv8_ouA7QUm-BgD9GoTLO5uqxhVu-CpffAUBk_tgqduUPPqbuCpHaG7V_xMGgJ8D0RsuSWEhz_6u-sPlwnjRg</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Yao, Weifeng</creator><creator>Gu, 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plasma and urine metabolomics coupled with HPLC/QTOF-MS and chemometric analysis on potential biomarkers in liver injury and hepatoprotective effects of Er-Zhi-Wan</title><author>Yao, Weifeng ; Gu, Haiwei ; Zhu, Jiangjiang ; Barding, Gregory ; Cheng, Haibo ; Bao, Beihua ; Zhang, Li ; Ding, Anwei ; Li, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c575t-f58451ab6d055ecb3f70877254ba3c9ab6cf072c941029ac8da85e278e310f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological markers</topic><topic>Biomarkers</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - urine</topic><topic>Biosynthesis</topic><topic>Carbon tetrachloride</topic><topic>Carbon Tetrachloride - toxicity</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical and Drug Induced Liver Injury - blood</topic><topic>Chemical and Drug Induced Liver Injury - diagnosis</topic><topic>Chemical and Drug Induced Liver Injury - prevention & control</topic><topic>Chemical and Drug Induced Liver Injury - urine</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemometrics</topic><topic>Chinese medicine</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Discriminant Analysis</topic><topic>Food Science</topic><topic>Glycine</topic><topic>Herbal medicine</topic><topic>High performance liquid chromatography</topic><topic>Injuries</topic><topic>Injury analysis</topic><topic>Injury prevention</topic><topic>Intoxication</topic><topic>Laboratory Medicine</topic><topic>Lipid metabolism</topic><topic>Liquid chromatography</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Lysine</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Mathematical models</topic><topic>Medicine, Chinese Traditional</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Metabolomics - methods</topic><topic>Molecular modelling</topic><topic>Monitoring/Environmental Analysis</topic><topic>Pathways</topic><topic>Pentose</topic><topic>Pentose phosphate pathway</topic><topic>Phosphoribosyl pyrophosphate</topic><topic>Principal components analysis</topic><topic>Quadrupoles</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Research Paper</topic><topic>Serine</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Sphinganine</topic><topic>Steroids</topic><topic>Threonine</topic><topic>Traditional Chinese medicine</topic><topic>Tryptophan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yao, Weifeng</creatorcontrib><creatorcontrib>Gu, Haiwei</creatorcontrib><creatorcontrib>Zhu, 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Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yao, Weifeng</au><au>Gu, Haiwei</au><au>Zhu, Jiangjiang</au><au>Barding, Gregory</au><au>Cheng, Haibo</au><au>Bao, Beihua</au><au>Zhang, Li</au><au>Ding, Anwei</au><au>Li, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated plasma and urine metabolomics coupled with HPLC/QTOF-MS and chemometric analysis on potential biomarkers in liver injury and hepatoprotective effects of Er-Zhi-Wan</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>406</volume><issue>28</issue><spage>7367</spage><epage>7378</epage><pages>7367-7378</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Metabolomics techniques are the comprehensive assessment of endogenous metabolites in a biological system and may provide additional insight into the molecular mechanisms. Er-Zhi-Wan (EZW) is a traditional Chinese medicine formula, which contains
Fructus Ligustri Lucidi
(FLL) and
Herba Ecliptae
(HE). EZW is widely used to prevent and treat various liver injuries through the nourishment of the liver. However, the precise molecular mechanism of hepatoprotective effects has not been comprehensively explored. Here, an integrated metabolomics strategy was designed to assess the effects and possible mechanisms of EZW against carbon tetrachloride-induced liver injury, a commonly used model of both acute and chronic liver intoxication. High-performance chromatography/quadrupole time-of-flight mass spectrometry (HPLC/QTOF-MS) combined with chemometric approaches including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to discover differentiating metabolites in metabolomics data of rat plasma and urine. Results indicate six differentiating metabolites, tryptophan, sphinganine, tetrahydrocorticosterone, pipecolic acid,
l
-2-amino-3-oxobutanoic acid and phosphoribosyl pyrophosphate, in the positive mode. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan metabolism, sphingolipid metabolism, steroid hormone biosynthesis, lysine degradation, glycine, serine and threonine metabolism, and pentose phosphate pathway. Of note, EZW has a potential pharmacological effect, which might be through regulating multiple perturbed pathways to the normal state. Our findings also showed that the robust integrated metabolomics techniques are promising for identifying more biomarkers and pathways and helping to clarify the function mechanisms of traditional Chinese medicine.
Graphical Abstract
Overview of the integrated metabolomics strategy</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25245419</pmid><doi>10.1007/s00216-014-8169-x</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2014-11, Vol.406 (28), p.7367-7378 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1746890024 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analysis Analytical Chemistry Animals Biochemistry Biological markers Biomarkers Biomarkers - blood Biomarkers - urine Biosynthesis Carbon tetrachloride Carbon Tetrachloride - toxicity Characterization and Evaluation of Materials Chemical and Drug Induced Liver Injury - blood Chemical and Drug Induced Liver Injury - diagnosis Chemical and Drug Induced Liver Injury - prevention & control Chemical and Drug Induced Liver Injury - urine Chemistry Chemistry and Materials Science Chemometrics Chinese medicine Chromatography, High Pressure Liquid Discriminant Analysis Food Science Glycine Herbal medicine High performance liquid chromatography Injuries Injury analysis Injury prevention Intoxication Laboratory Medicine Lipid metabolism Liquid chromatography Liver Liver - drug effects Liver - metabolism Lysine Male Mass spectrometry Mass spectroscopy Mathematical models Medicine, Chinese Traditional Metabolism Metabolites Metabolomics Metabolomics - methods Molecular modelling Monitoring/Environmental Analysis Pathways Pentose Pentose phosphate pathway Phosphoribosyl pyrophosphate Principal components analysis Quadrupoles Rats Rats, Wistar Research Paper Serine Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Sphinganine Steroids Threonine Traditional Chinese medicine Tryptophan |
| title | Integrated plasma and urine metabolomics coupled with HPLC/QTOF-MS and chemometric analysis on potential biomarkers in liver injury and hepatoprotective effects of Er-Zhi-Wan |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T02%3A44%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Integrated%20plasma%20and%20urine%20metabolomics%20coupled%20with%20HPLC/QTOF-MS%20and%20chemometric%20analysis%20on%20potential%20biomarkers%20in%20liver%20injury%20and%20hepatoprotective%20effects%20of%20Er-Zhi-Wan&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Yao,%20Weifeng&rft.date=2014-11-01&rft.volume=406&rft.issue=28&rft.spage=7367&rft.epage=7378&rft.pages=7367-7378&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-014-8169-x&rft_dat=%3Cgale_proqu%3EA389645454%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2511567572&rft_id=info:pmid/25245419&rft_galeid=A389645454&rfr_iscdi=true |