Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells
Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induc...
Gespeichert in:
| Veröffentlicht in: | Biologicals 2015-09, Vol.43 (5), p.349-354 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 354 |
|---|---|
| container_issue | 5 |
| container_start_page | 349 |
| container_title | Biologicals |
| container_volume | 43 |
| creator | Eslami-Arshaghi, Tarlan Salehi, Mohammad Soleimani, Masoud Gholipourmalekabadi, Mazaher Mossahebi-Mohammadi, Majid Ardeshirylajimi, Abdolreza Rajabi, Hoda |
| description | Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors. |
| doi_str_mv | 10.1016/j.biologicals.2015.07.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1746883230</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1045105615000810</els_id><sourcerecordid>1746883230</sourcerecordid><originalsourceid>FETCH-LOGICAL-c480t-fbf286925d28254d3aa8244616b40cd2e68a76146931d0eedaebfa0c716140a3</originalsourceid><addsrcrecordid>eNqNkTFv2zAQhYmiRZ04-QsFu3WReqQoihoLI2kCGOjipRNBkSeXhiS6pBzA_z507QYZPfF4-O7u4T1CvjIoGTD5fVd2Pgxh660ZUsmB1SU0JQD7QG4YtHWhKg4fT7WoCwa1XJDblHYZYKIRn8mCS161slE35Pf6OO7_BO_o4Cc0W6TO9z1GnGZvZh8mug_zv89AQ0_HcEhIp4Md0EQ6RzOlDFMcu3gMk7c0zThSi8OQ7sinPsvD-8u7JJvHh83qqVj_-vm8-rEurFAwF33XcyVbXjuueC1cZYziQkgmOwHWcZTKNJIJ2VbMAaIz2PUGbMNyE0y1JN_Oa_cx_D1gmvXo00mAmTCL1awRUmU_KrgCZVXNFbRNRtszamNIKWKv99GPJh41A33KQO_0uwz0KQMNjc4W59kvlzOHbkT3Nvnf9AyszgBmW148Rp2sx8mi8xHtrF3wV5x5BYoonvE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1713528097</pqid></control><display><type>article</type><title>Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Eslami-Arshaghi, Tarlan ; Salehi, Mohammad ; Soleimani, Masoud ; Gholipourmalekabadi, Mazaher ; Mossahebi-Mohammadi, Majid ; Ardeshirylajimi, Abdolreza ; Rajabi, Hoda</creator><creatorcontrib>Eslami-Arshaghi, Tarlan ; Salehi, Mohammad ; Soleimani, Masoud ; Gholipourmalekabadi, Mazaher ; Mossahebi-Mohammadi, Majid ; Ardeshirylajimi, Abdolreza ; Rajabi, Hoda</creatorcontrib><description>Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors.</description><identifier>ISSN: 1045-1056</identifier><identifier>EISSN: 1095-8320</identifier><identifier>DOI: 10.1016/j.biologicals.2015.07.001</identifier><identifier>PMID: 26239678</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antigens, CD - immunology ; Cell Differentiation ; Cell Lineage ; Cells, Cultured ; Embryonic Stem Cells - cytology ; Flow Cytometry ; Growth factors ; Lymphoid Tissue - cytology ; Lymphoid Tissue - immunology ; Mice ; Nuclear transfer embryonic stem cells ; Nuclear Transfer Techniques ; Somatic cell nuclear transfer ; T and B lymphocytes</subject><ispartof>Biologicals, 2015-09, Vol.43 (5), p.349-354</ispartof><rights>2015 The International Alliance for Biological Standardization</rights><rights>Copyright © 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-fbf286925d28254d3aa8244616b40cd2e68a76146931d0eedaebfa0c716140a3</citedby><cites>FETCH-LOGICAL-c480t-fbf286925d28254d3aa8244616b40cd2e68a76146931d0eedaebfa0c716140a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1045105615000810$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26239678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eslami-Arshaghi, Tarlan</creatorcontrib><creatorcontrib>Salehi, Mohammad</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Gholipourmalekabadi, Mazaher</creatorcontrib><creatorcontrib>Mossahebi-Mohammadi, Majid</creatorcontrib><creatorcontrib>Ardeshirylajimi, Abdolreza</creatorcontrib><creatorcontrib>Rajabi, Hoda</creatorcontrib><title>Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells</title><title>Biologicals</title><addtitle>Biologicals</addtitle><description>Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors.</description><subject>Animals</subject><subject>Antigens, CD - immunology</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Cells, Cultured</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Flow Cytometry</subject><subject>Growth factors</subject><subject>Lymphoid Tissue - cytology</subject><subject>Lymphoid Tissue - immunology</subject><subject>Mice</subject><subject>Nuclear transfer embryonic stem cells</subject><subject>Nuclear Transfer Techniques</subject><subject>Somatic cell nuclear transfer</subject><subject>T and B lymphocytes</subject><issn>1045-1056</issn><issn>1095-8320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTFv2zAQhYmiRZ04-QsFu3WReqQoihoLI2kCGOjipRNBkSeXhiS6pBzA_z507QYZPfF4-O7u4T1CvjIoGTD5fVd2Pgxh660ZUsmB1SU0JQD7QG4YtHWhKg4fT7WoCwa1XJDblHYZYKIRn8mCS161slE35Pf6OO7_BO_o4Cc0W6TO9z1GnGZvZh8mug_zv89AQ0_HcEhIp4Md0EQ6RzOlDFMcu3gMk7c0zThSi8OQ7sinPsvD-8u7JJvHh83qqVj_-vm8-rEurFAwF33XcyVbXjuueC1cZYziQkgmOwHWcZTKNJIJ2VbMAaIz2PUGbMNyE0y1JN_Oa_cx_D1gmvXo00mAmTCL1awRUmU_KrgCZVXNFbRNRtszamNIKWKv99GPJh41A33KQO_0uwz0KQMNjc4W59kvlzOHbkT3Nvnf9AyszgBmW148Rp2sx8mi8xHtrF3wV5x5BYoonvE</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Eslami-Arshaghi, Tarlan</creator><creator>Salehi, Mohammad</creator><creator>Soleimani, Masoud</creator><creator>Gholipourmalekabadi, Mazaher</creator><creator>Mossahebi-Mohammadi, Majid</creator><creator>Ardeshirylajimi, Abdolreza</creator><creator>Rajabi, Hoda</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20150901</creationdate><title>Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells</title><author>Eslami-Arshaghi, Tarlan ; Salehi, Mohammad ; Soleimani, Masoud ; Gholipourmalekabadi, Mazaher ; Mossahebi-Mohammadi, Majid ; Ardeshirylajimi, Abdolreza ; Rajabi, Hoda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-fbf286925d28254d3aa8244616b40cd2e68a76146931d0eedaebfa0c716140a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antigens, CD - immunology</topic><topic>Cell Differentiation</topic><topic>Cell Lineage</topic><topic>Cells, Cultured</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Flow Cytometry</topic><topic>Growth factors</topic><topic>Lymphoid Tissue - cytology</topic><topic>Lymphoid Tissue - immunology</topic><topic>Mice</topic><topic>Nuclear transfer embryonic stem cells</topic><topic>Nuclear Transfer Techniques</topic><topic>Somatic cell nuclear transfer</topic><topic>T and B lymphocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eslami-Arshaghi, Tarlan</creatorcontrib><creatorcontrib>Salehi, Mohammad</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Gholipourmalekabadi, Mazaher</creatorcontrib><creatorcontrib>Mossahebi-Mohammadi, Majid</creatorcontrib><creatorcontrib>Ardeshirylajimi, Abdolreza</creatorcontrib><creatorcontrib>Rajabi, Hoda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Biologicals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eslami-Arshaghi, Tarlan</au><au>Salehi, Mohammad</au><au>Soleimani, Masoud</au><au>Gholipourmalekabadi, Mazaher</au><au>Mossahebi-Mohammadi, Majid</au><au>Ardeshirylajimi, Abdolreza</au><au>Rajabi, Hoda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells</atitle><jtitle>Biologicals</jtitle><addtitle>Biologicals</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>43</volume><issue>5</issue><spage>349</spage><epage>354</epage><pages>349-354</pages><issn>1045-1056</issn><eissn>1095-8320</eissn><abstract>Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26239678</pmid><doi>10.1016/j.biologicals.2015.07.001</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1045-1056 |
| ispartof | Biologicals, 2015-09, Vol.43 (5), p.349-354 |
| issn | 1045-1056 1095-8320 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1746883230 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antigens, CD - immunology Cell Differentiation Cell Lineage Cells, Cultured Embryonic Stem Cells - cytology Flow Cytometry Growth factors Lymphoid Tissue - cytology Lymphoid Tissue - immunology Mice Nuclear transfer embryonic stem cells Nuclear Transfer Techniques Somatic cell nuclear transfer T and B lymphocytes |
| title | Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T02%3A26%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lymphoid%20lineage%20differentiation%20potential%20of%20mouse%20nuclear%20transfer%20embryonic%20stem%20cells&rft.jtitle=Biologicals&rft.au=Eslami-Arshaghi,%20Tarlan&rft.date=2015-09-01&rft.volume=43&rft.issue=5&rft.spage=349&rft.epage=354&rft.pages=349-354&rft.issn=1045-1056&rft.eissn=1095-8320&rft_id=info:doi/10.1016/j.biologicals.2015.07.001&rft_dat=%3Cproquest_cross%3E1746883230%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1713528097&rft_id=info:pmid/26239678&rft_els_id=S1045105615000810&rfr_iscdi=true |