Real time kinetic analysis of the interaction between interleukin-1α and soluble interleukin-1 receptor I using a resonant mirror biosensor
A novel method for real time kinetic analysis of the interaction between IL-1α and sIL-1R I is reported. sIL-1R I was immobilized on the biotin cuvette surface of the resonant mirror biosensor to set up the experimental model. Results obtained from the assay confirmed that biotinylated sIL-1R I was...
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| Veröffentlicht in: | Analytica chimica acta 2006-01, Vol.557 (1), p.106-110 |
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| container_title | Analytica chimica acta |
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| creator | Wu, Bao-Yan Li, Jing Huang, Jia-Dong Wang, Yan-Yan Yin, Hui-Jun Chen, Ke-Ji Chen, Qiang |
| description | A novel method for real time kinetic analysis of the interaction between IL-1α and sIL-1R I is reported. sIL-1R I was immobilized on the biotin cuvette surface of the resonant mirror biosensor to set up the experimental model. Results obtained from the assay confirmed that biotinylated sIL-1R I was specifically immobilized on the avidin-coated biotin cuvette surface, the interaction between IL-1α and sIL-1R I was fast and specific, and the interaction response is dose-dependence of IL-1α in solution with a range of 150–2400
nM. The binding curve was fitted by FASTfit analysis, which fitted the monophasic association quite well, and the error did not exceed 0.4 arc seconds. Kinetic constants for IL-1α binding to sIL-1R I were determined from the plots of
K
on versus the concentration of IL-1α. For the interaction,
k
ass was 2.81
×
10
3
M
−1
s
−1,
K
diss was 2.52
×
10
−3
s
−1, and
K
D was 8.97
×
10
−7
M. |
| doi_str_mv | 10.1016/j.aca.2005.09.075 |
| format | Article |
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nM. The binding curve was fitted by FASTfit analysis, which fitted the monophasic association quite well, and the error did not exceed 0.4 arc seconds. Kinetic constants for IL-1α binding to sIL-1R I were determined from the plots of
K
on versus the concentration of IL-1α. For the interaction,
k
ass was 2.81
×
10
3
M
−1
s
−1,
K
diss was 2.52
×
10
−3
s
−1, and
K
D was 8.97
×
10
−7
M.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2005.09.075</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Biological and medical sciences ; Biosensors ; Biotechnology ; Chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Interleukin-1α ; Kinetic analysis ; Methods. Procedures. Technologies ; Resonant mirror biosensor ; Soluble interleukin-1 receptor I ; Various methods and equipments</subject><ispartof>Analytica chimica acta, 2006-01, Vol.557 (1), p.106-110</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-6e325bec3be53561f3a70234d71c1e88ed2853495bd0418ed1a45c6a3a805c983</citedby><cites>FETCH-LOGICAL-c358t-6e325bec3be53561f3a70234d71c1e88ed2853495bd0418ed1a45c6a3a805c983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2005.09.075$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17577833$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Bao-Yan</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Huang, Jia-Dong</creatorcontrib><creatorcontrib>Wang, Yan-Yan</creatorcontrib><creatorcontrib>Yin, Hui-Jun</creatorcontrib><creatorcontrib>Chen, Ke-Ji</creatorcontrib><creatorcontrib>Chen, Qiang</creatorcontrib><title>Real time kinetic analysis of the interaction between interleukin-1α and soluble interleukin-1 receptor I using a resonant mirror biosensor</title><title>Analytica chimica acta</title><description>A novel method for real time kinetic analysis of the interaction between IL-1α and sIL-1R I is reported. sIL-1R I was immobilized on the biotin cuvette surface of the resonant mirror biosensor to set up the experimental model. Results obtained from the assay confirmed that biotinylated sIL-1R I was specifically immobilized on the avidin-coated biotin cuvette surface, the interaction between IL-1α and sIL-1R I was fast and specific, and the interaction response is dose-dependence of IL-1α in solution with a range of 150–2400
nM. The binding curve was fitted by FASTfit analysis, which fitted the monophasic association quite well, and the error did not exceed 0.4 arc seconds. Kinetic constants for IL-1α binding to sIL-1R I were determined from the plots of
K
on versus the concentration of IL-1α. For the interaction,
k
ass was 2.81
×
10
3
M
−1
s
−1,
K
diss was 2.52
×
10
−3
s
−1, and
K
D was 8.97
×
10
−7
M.</description><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Interleukin-1α</subject><subject>Kinetic analysis</subject><subject>Methods. Procedures. Technologies</subject><subject>Resonant mirror biosensor</subject><subject>Soluble interleukin-1 receptor I</subject><subject>Various methods and equipments</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kMFu1DAQhi0EEsvSB-DmC9wS7DiOs-KEKiiVKlVC9GxNnAl48dqLxwH1HXgZXoRnqquthHrhNPpn_n9G8zH2SopWCjm83bfgoO2E0K3YtcLoJ2wjR6OaXnX9U7YRQqimG4x4zl4Q7avspOg37PdnhMCLPyD_7iMW7zhECLfkiaeFl2_IfSyYwRWfIp-w_EKMp17AtWYa-fdPzcycUlingI9nPKPDY0mZX_KVfPzKobYoRYiFH3zOdTL5RBgp5Zfs2QKB8OyhbtnNxw9fzj81V9cXl-fvrxqn9FiaAVWnJ3RqQq30IBcFRnSqn410EscR527Uqt_paRa9rFJCr90ACkah3W5UW_bmtPeY048VqdiDJ4chQMS0kpWmH3pR6W2ZPBldTkQZF3vM_gD51kph77nbva3c7T13K3a2cq-Z1w_LgRyEJUN0nv4FjTZmVKr63p18WD_96TFbch6jw9lXZsXOyf_nyh0fBZry</recordid><startdate>20060131</startdate><enddate>20060131</enddate><creator>Wu, Bao-Yan</creator><creator>Li, Jing</creator><creator>Huang, Jia-Dong</creator><creator>Wang, Yan-Yan</creator><creator>Yin, Hui-Jun</creator><creator>Chen, Ke-Ji</creator><creator>Chen, Qiang</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20060131</creationdate><title>Real time kinetic analysis of the interaction between interleukin-1α and soluble interleukin-1 receptor I using a resonant mirror biosensor</title><author>Wu, Bao-Yan ; Li, Jing ; Huang, Jia-Dong ; Wang, Yan-Yan ; Yin, Hui-Jun ; Chen, Ke-Ji ; Chen, Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-6e325bec3be53561f3a70234d71c1e88ed2853495bd0418ed1a45c6a3a805c983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Interleukin-1α</topic><topic>Kinetic analysis</topic><topic>Methods. Procedures. Technologies</topic><topic>Resonant mirror biosensor</topic><topic>Soluble interleukin-1 receptor I</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Bao-Yan</creatorcontrib><creatorcontrib>Li, Jing</creatorcontrib><creatorcontrib>Huang, Jia-Dong</creatorcontrib><creatorcontrib>Wang, Yan-Yan</creatorcontrib><creatorcontrib>Yin, Hui-Jun</creatorcontrib><creatorcontrib>Chen, Ke-Ji</creatorcontrib><creatorcontrib>Chen, Qiang</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Bao-Yan</au><au>Li, Jing</au><au>Huang, Jia-Dong</au><au>Wang, Yan-Yan</au><au>Yin, Hui-Jun</au><au>Chen, Ke-Ji</au><au>Chen, Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real time kinetic analysis of the interaction between interleukin-1α and soluble interleukin-1 receptor I using a resonant mirror biosensor</atitle><jtitle>Analytica chimica acta</jtitle><date>2006-01-31</date><risdate>2006</risdate><volume>557</volume><issue>1</issue><spage>106</spage><epage>110</epage><pages>106-110</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>A novel method for real time kinetic analysis of the interaction between IL-1α and sIL-1R I is reported. sIL-1R I was immobilized on the biotin cuvette surface of the resonant mirror biosensor to set up the experimental model. Results obtained from the assay confirmed that biotinylated sIL-1R I was specifically immobilized on the avidin-coated biotin cuvette surface, the interaction between IL-1α and sIL-1R I was fast and specific, and the interaction response is dose-dependence of IL-1α in solution with a range of 150–2400
nM. The binding curve was fitted by FASTfit analysis, which fitted the monophasic association quite well, and the error did not exceed 0.4 arc seconds. Kinetic constants for IL-1α binding to sIL-1R I were determined from the plots of
K
on versus the concentration of IL-1α. For the interaction,
k
ass was 2.81
×
10
3
M
−1
s
−1,
K
diss was 2.52
×
10
−3
s
−1, and
K
D was 8.97
×
10
−7
M.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.aca.2005.09.075</doi><tpages>5</tpages></addata></record> |
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| subjects | Analytical chemistry Biological and medical sciences Biosensors Biotechnology Chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Interleukin-1α Kinetic analysis Methods. Procedures. Technologies Resonant mirror biosensor Soluble interleukin-1 receptor I Various methods and equipments |
| title | Real time kinetic analysis of the interaction between interleukin-1α and soluble interleukin-1 receptor I using a resonant mirror biosensor |
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