Genotyping Measles Virus by Real-Time Amplification Refractory Mutation System PCR Represents a Rapid Approach for Measles Outbreak Investigations
Real-time PCR has been developed to genotype measles virus (MV) isolates. MV strains circulating in epidemics in Gabon in 1984, Cameroon in 2001, Morocco in 2003, and France in 2004 were investigated. We developed a real-time amplification refractory mutation system PCR (RT-AMRS PCR) using SYBR gree...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2006-02, Vol.44 (2), p.487-494 |
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| creator | Waku-Kouomou, Diane Alla, Amal Blanquier, Bariza Jeantet, Damien Caidi, Hayat Rguig, Ahmed Freymuth, François Wild, Fabian T |
| description | Real-time PCR has been developed to genotype measles virus (MV) isolates. MV strains circulating in epidemics in Gabon in 1984, Cameroon in 2001, Morocco in 2003, and France in 2004 were investigated. We developed a real-time amplification refractory mutation system PCR (RT-AMRS PCR) using SYBR green fluorescent dye. Six pairs of primers for RT-ARMS PCR were designed to specifically amplify genotypes A, B2, B3.1, B3.2, C2, and D7. Genotypes could be differentiated by melting curve analysis. All strains were also confirmed by direct sequencing. Using the result obtained by direct sequencing and phylogenetic analysis as the reference, the accuracy of MV by RT-ARMS PCR and melting curve analysis was 97%. However, the latter method is more rapid and sensitive than the former method. This method could be a useful tool for molecular epidemiological studies of MV, providing an efficient alternative for large-scale studies. |
| doi_str_mv | 10.1128/JCM.44.2.487-494.2006 |
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MV strains circulating in epidemics in Gabon in 1984, Cameroon in 2001, Morocco in 2003, and France in 2004 were investigated. We developed a real-time amplification refractory mutation system PCR (RT-AMRS PCR) using SYBR green fluorescent dye. Six pairs of primers for RT-ARMS PCR were designed to specifically amplify genotypes A, B2, B3.1, B3.2, C2, and D7. Genotypes could be differentiated by melting curve analysis. All strains were also confirmed by direct sequencing. Using the result obtained by direct sequencing and phylogenetic analysis as the reference, the accuracy of MV by RT-ARMS PCR and melting curve analysis was 97%. However, the latter method is more rapid and sensitive than the former method. This method could be a useful tool for molecular epidemiological studies of MV, providing an efficient alternative for large-scale studies.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JCM.44.2.487-494.2006</identifier><identifier>PMID: 16455903</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Biological and medical sciences ; Disease Outbreaks ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Genotype ; Human viral diseases ; Humans ; Infectious diseases ; Measles - epidemiology ; Measles - virology ; Measles virus ; Measles virus - classification ; Measles virus - genetics ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Mutation ; Organic Chemicals ; Polymerase Chain Reaction - methods ; Reproducibility of Results ; Sequence Analysis, DNA ; Time Factors ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye ; Virology</subject><ispartof>Journal of Clinical Microbiology, 2006-02, Vol.44 (2), p.487-494</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-2d371e6a4f6c8c9fdec8cbfe0887e5442e3b5ba1387bc54d9f1d8bd51a5013413</citedby><cites>FETCH-LOGICAL-c519t-2d371e6a4f6c8c9fdec8cbfe0887e5442e3b5ba1387bc54d9f1d8bd51a5013413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1392642/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1392642/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17486713$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16455903$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Waku-Kouomou, Diane</creatorcontrib><creatorcontrib>Alla, Amal</creatorcontrib><creatorcontrib>Blanquier, Bariza</creatorcontrib><creatorcontrib>Jeantet, Damien</creatorcontrib><creatorcontrib>Caidi, Hayat</creatorcontrib><creatorcontrib>Rguig, Ahmed</creatorcontrib><creatorcontrib>Freymuth, François</creatorcontrib><creatorcontrib>Wild, Fabian T</creatorcontrib><title>Genotyping Measles Virus by Real-Time Amplification Refractory Mutation System PCR Represents a Rapid Approach for Measles Outbreak Investigations</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Real-time PCR has been developed to genotype measles virus (MV) isolates. MV strains circulating in epidemics in Gabon in 1984, Cameroon in 2001, Morocco in 2003, and France in 2004 were investigated. We developed a real-time amplification refractory mutation system PCR (RT-AMRS PCR) using SYBR green fluorescent dye. Six pairs of primers for RT-ARMS PCR were designed to specifically amplify genotypes A, B2, B3.1, B3.2, C2, and D7. Genotypes could be differentiated by melting curve analysis. All strains were also confirmed by direct sequencing. Using the result obtained by direct sequencing and phylogenetic analysis as the reference, the accuracy of MV by RT-ARMS PCR and melting curve analysis was 97%. However, the latter method is more rapid and sensitive than the former method. This method could be a useful tool for molecular epidemiological studies of MV, providing an efficient alternative for large-scale studies.</description><subject>Biological and medical sciences</subject><subject>Disease Outbreaks</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Measles - epidemiology</subject><subject>Measles - virology</subject><subject>Measles virus</subject><subject>Measles virus - classification</subject><subject>Measles virus - genetics</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Organic Chemicals</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>Sequence Analysis, DNA</subject><subject>Time Factors</subject><subject>Viral diseases</subject><subject>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><subject>Virology</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEotvCIwC-0FsWO_4T54K0WkEp6qpo2yJuluNMdl2SONhJUV6DJ8bLrrZw4jTWzG--mfGXJK8InhOSyXefl6s5Y_NszmSesiK-MBZPkhnBhUyFwN-eJjOMC54SQvOT5DSEe4wJY5w_T06IiLHAdJb8uoDODVNvuw1agQ4NBPTV-jGgckJr0E16a1tAi7ZvbG2NHqzrYr722gzOT2g1DvvczRQGaNGX5TqWew8BuiEgjda6txVa9L132mxR7fxxzvU4lB70d3TZPUAY7OaPUniRPKt1E-DlIZ4ldx8_3C4_pVfXF5fLxVVqOCmGNKtoTkBoVgsjTVFXEENZA5YyB85YBrTkpSZU5qXhrCpqUsmy4kRzTCgj9Cx5v9ftx7KFysSFvW5U722r_aScturfSme3auMeFKFFJlgWBc4PAt79GOMFqrXBQNPoDtwYlMhFJmQu_guSnAlaSBxBvgeNdyF4qI_bEKx2tqtou2JMZSrarqLtamd77Hv99ymPXQefI_D2AOhgdBPt64wNj1zOpMjJjkN7bms325_Wg9KhVfemPQ6NyJs9Umun9MZHmbubLH4pJphngjL6GxT3zko</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Waku-Kouomou, Diane</creator><creator>Alla, Amal</creator><creator>Blanquier, Bariza</creator><creator>Jeantet, Damien</creator><creator>Caidi, Hayat</creator><creator>Rguig, Ahmed</creator><creator>Freymuth, François</creator><creator>Wild, Fabian T</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060201</creationdate><title>Genotyping Measles Virus by Real-Time Amplification Refractory Mutation System PCR Represents a Rapid Approach for Measles Outbreak Investigations</title><author>Waku-Kouomou, Diane ; Alla, Amal ; Blanquier, Bariza ; Jeantet, Damien ; Caidi, Hayat ; Rguig, Ahmed ; Freymuth, François ; Wild, Fabian T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-2d371e6a4f6c8c9fdec8cbfe0887e5442e3b5ba1387bc54d9f1d8bd51a5013413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>Disease Outbreaks</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Measles - epidemiology</topic><topic>Measles - virology</topic><topic>Measles virus</topic><topic>Measles virus - classification</topic><topic>Measles virus - genetics</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Organic Chemicals</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reproducibility of Results</topic><topic>Sequence Analysis, DNA</topic><topic>Time Factors</topic><topic>Viral diseases</topic><topic>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Waku-Kouomou, Diane</creatorcontrib><creatorcontrib>Alla, Amal</creatorcontrib><creatorcontrib>Blanquier, Bariza</creatorcontrib><creatorcontrib>Jeantet, Damien</creatorcontrib><creatorcontrib>Caidi, Hayat</creatorcontrib><creatorcontrib>Rguig, Ahmed</creatorcontrib><creatorcontrib>Freymuth, François</creatorcontrib><creatorcontrib>Wild, Fabian T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Waku-Kouomou, Diane</au><au>Alla, Amal</au><au>Blanquier, Bariza</au><au>Jeantet, Damien</au><au>Caidi, Hayat</au><au>Rguig, Ahmed</au><au>Freymuth, François</au><au>Wild, Fabian T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping Measles Virus by Real-Time Amplification Refractory Mutation System PCR Represents a Rapid Approach for Measles Outbreak Investigations</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>44</volume><issue>2</issue><spage>487</spage><epage>494</epage><pages>487-494</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>Real-time PCR has been developed to genotype measles virus (MV) isolates. MV strains circulating in epidemics in Gabon in 1984, Cameroon in 2001, Morocco in 2003, and France in 2004 were investigated. We developed a real-time amplification refractory mutation system PCR (RT-AMRS PCR) using SYBR green fluorescent dye. Six pairs of primers for RT-ARMS PCR were designed to specifically amplify genotypes A, B2, B3.1, B3.2, C2, and D7. Genotypes could be differentiated by melting curve analysis. All strains were also confirmed by direct sequencing. Using the result obtained by direct sequencing and phylogenetic analysis as the reference, the accuracy of MV by RT-ARMS PCR and melting curve analysis was 97%. However, the latter method is more rapid and sensitive than the former method. This method could be a useful tool for molecular epidemiological studies of MV, providing an efficient alternative for large-scale studies.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16455903</pmid><doi>10.1128/JCM.44.2.487-494.2006</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Disease Outbreaks Fluorescent Dyes Fundamental and applied biological sciences. Psychology Genotype Human viral diseases Humans Infectious diseases Measles - epidemiology Measles - virology Measles virus Measles virus - classification Measles virus - genetics Medical sciences Microbiology Miscellaneous Molecular Sequence Data Mutation Organic Chemicals Polymerase Chain Reaction - methods Reproducibility of Results Sequence Analysis, DNA Time Factors Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye Virology |
| title | Genotyping Measles Virus by Real-Time Amplification Refractory Mutation System PCR Represents a Rapid Approach for Measles Outbreak Investigations |
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