Identification of new nodulin cDNAs from yellow lupine by differential display
In this study several sequences that represent yellow lupine nodulin genes are identified. Four different cDNA clones were isolated from a lupine nodule cDNA library and sequenced. Database search revealed that the deduced amino acid of one clone, DD3A20, shows near 80% amino acid similarity to plan...
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| Veröffentlicht in: | Plant science (Limerick) 2000-02, Vol.151 (1), p.75-83 |
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| creator | Swiderski, Michal R Zaborowska, Zaneta Legocki, Andrzej B |
| description | In this study several sequences that represent yellow lupine nodulin genes are identified. Four different cDNA clones were isolated from a lupine nodule cDNA library and sequenced. Database search revealed that the deduced amino acid of one clone, DD3A20, shows near 80% amino acid similarity to plant stearoyl-acyl carrier protein desaturases and contains crucial amino acids in the fatty acid binding domain which are characteristic for stearoyl-ACP desaturases. The other sequences seem to be unique. Nearly identical signal peptide sequences are present at the N-termini of the DD4A9 and DD2T15 putative proteins. Northern analysis indicated that the pattern of expression of the
Lldd4A9,
Lldd2A18 and
Lldd2T15 genes is the same as that of the lupine leghemoglobin gene
LllbII, while the desaturase gene
Ll3A20 is turned on at least 3 days earlier.
Ll3A20 is also expressed in lupine fruits. RT-PCR analysis of the
Lldd3A20 and
Lldd2T15 expression confirmed organ-specific and temporal pattern of expression in nodules. |
| doi_str_mv | 10.1016/S0168-9452(99)00198-3 |
| format | Article |
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Lldd4A9,
Lldd2A18 and
Lldd2T15 genes is the same as that of the lupine leghemoglobin gene
LllbII, while the desaturase gene
Ll3A20 is turned on at least 3 days earlier.
Ll3A20 is also expressed in lupine fruits. RT-PCR analysis of the
Lldd3A20 and
Lldd2T15 expression confirmed organ-specific and temporal pattern of expression in nodules.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/S0168-9452(99)00198-3</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Desaturase ; Economic plant physiology ; Fundamental and applied biological sciences. Psychology ; Ll3A20 gene ; Lldd2A18 gene ; Lldd2T15 gene ; Lldd3A20 gene ; Lldd4A9 gene ; LllbII gene ; Lupinus ; Nodules ; Nodulins ; Parasitism and symbiosis ; Plant physiology and development ; Symbiosis ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; Yellow lupine</subject><ispartof>Plant science (Limerick), 2000-02, Vol.151 (1), p.75-83</ispartof><rights>2000 Elsevier Science Ireland Ltd</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-975942c3192b53ac74d73a845aa2d9eec36e57afb7009550b881b94464177aa93</citedby><cites>FETCH-LOGICAL-c367t-975942c3192b53ac74d73a845aa2d9eec36e57afb7009550b881b94464177aa93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945299001983$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1249434$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Swiderski, Michal R</creatorcontrib><creatorcontrib>Zaborowska, Zaneta</creatorcontrib><creatorcontrib>Legocki, Andrzej B</creatorcontrib><title>Identification of new nodulin cDNAs from yellow lupine by differential display</title><title>Plant science (Limerick)</title><description>In this study several sequences that represent yellow lupine nodulin genes are identified. Four different cDNA clones were isolated from a lupine nodule cDNA library and sequenced. Database search revealed that the deduced amino acid of one clone, DD3A20, shows near 80% amino acid similarity to plant stearoyl-acyl carrier protein desaturases and contains crucial amino acids in the fatty acid binding domain which are characteristic for stearoyl-ACP desaturases. The other sequences seem to be unique. Nearly identical signal peptide sequences are present at the N-termini of the DD4A9 and DD2T15 putative proteins. Northern analysis indicated that the pattern of expression of the
Lldd4A9,
Lldd2A18 and
Lldd2T15 genes is the same as that of the lupine leghemoglobin gene
LllbII, while the desaturase gene
Ll3A20 is turned on at least 3 days earlier.
Ll3A20 is also expressed in lupine fruits. RT-PCR analysis of the
Lldd3A20 and
Lldd2T15 expression confirmed organ-specific and temporal pattern of expression in nodules.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Desaturase</subject><subject>Economic plant physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ll3A20 gene</subject><subject>Lldd2A18 gene</subject><subject>Lldd2T15 gene</subject><subject>Lldd3A20 gene</subject><subject>Lldd4A9 gene</subject><subject>LllbII gene</subject><subject>Lupinus</subject><subject>Nodules</subject><subject>Nodulins</subject><subject>Parasitism and symbiosis</subject><subject>Plant physiology and development</subject><subject>Symbiosis</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>Yellow lupine</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkE9LAzEQxYMoWKsfQchBRA-rySZpNicp9V-h1IN6DtnsBCLbpCZbS7-921b06GWGgd-bN_MQOqfkhhI6un3tS1UoLsorpa4Joaoq2AEa0EqyoiyFOkSDX-QYneT8QQgphZADNJ82EDrvvDWdjwFHhwOscYjNqvUB2_v5OGOX4gJvoG3jGrerpQ-A6w1uvHOQtmrT9kNetmZzio6caTOc_fQhen98eJs8F7OXp-lkPCssG8muUFIoXlpGVVkLZqzkjWSm4sKYslEAPQVCGldLQpQQpK4qWivOR5xKaYxiQ3S537tM8XMFudMLn21_oQkQV1lTyUdUMdqDYg_aFHNO4PQy-YVJG02J3qand-npbTRaKb1LT7Ned_FjYLI1rUsmWJ__xCVXnPEeu9tj0D_75SHpbD0EC41PYDvdRP-P0Tct1IMv</recordid><startdate>20000207</startdate><enddate>20000207</enddate><creator>Swiderski, Michal R</creator><creator>Zaborowska, Zaneta</creator><creator>Legocki, Andrzej B</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000207</creationdate><title>Identification of new nodulin cDNAs from yellow lupine by differential display</title><author>Swiderski, Michal R ; Zaborowska, Zaneta ; Legocki, Andrzej B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-975942c3192b53ac74d73a845aa2d9eec36e57afb7009550b881b94464177aa93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Desaturase</topic><topic>Economic plant physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ll3A20 gene</topic><topic>Lldd2A18 gene</topic><topic>Lldd2T15 gene</topic><topic>Lldd3A20 gene</topic><topic>Lldd4A9 gene</topic><topic>LllbII gene</topic><topic>Lupinus</topic><topic>Nodules</topic><topic>Nodulins</topic><topic>Parasitism and symbiosis</topic><topic>Plant physiology and development</topic><topic>Symbiosis</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>Yellow lupine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swiderski, Michal R</creatorcontrib><creatorcontrib>Zaborowska, Zaneta</creatorcontrib><creatorcontrib>Legocki, Andrzej B</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swiderski, Michal R</au><au>Zaborowska, Zaneta</au><au>Legocki, Andrzej B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of new nodulin cDNAs from yellow lupine by differential display</atitle><jtitle>Plant science (Limerick)</jtitle><date>2000-02-07</date><risdate>2000</risdate><volume>151</volume><issue>1</issue><spage>75</spage><epage>83</epage><pages>75-83</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>In this study several sequences that represent yellow lupine nodulin genes are identified. Four different cDNA clones were isolated from a lupine nodule cDNA library and sequenced. Database search revealed that the deduced amino acid of one clone, DD3A20, shows near 80% amino acid similarity to plant stearoyl-acyl carrier protein desaturases and contains crucial amino acids in the fatty acid binding domain which are characteristic for stearoyl-ACP desaturases. The other sequences seem to be unique. Nearly identical signal peptide sequences are present at the N-termini of the DD4A9 and DD2T15 putative proteins. Northern analysis indicated that the pattern of expression of the
Lldd4A9,
Lldd2A18 and
Lldd2T15 genes is the same as that of the lupine leghemoglobin gene
LllbII, while the desaturase gene
Ll3A20 is turned on at least 3 days earlier.
Ll3A20 is also expressed in lupine fruits. RT-PCR analysis of the
Lldd3A20 and
Lldd2T15 expression confirmed organ-specific and temporal pattern of expression in nodules.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/S0168-9452(99)00198-3</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant science (Limerick), 2000-02, Vol.151 (1), p.75-83 |
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| source | Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Desaturase Economic plant physiology Fundamental and applied biological sciences. Psychology Ll3A20 gene Lldd2A18 gene Lldd2T15 gene Lldd3A20 gene Lldd4A9 gene LllbII gene Lupinus Nodules Nodulins Parasitism and symbiosis Plant physiology and development Symbiosis Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Yellow lupine |
| title | Identification of new nodulin cDNAs from yellow lupine by differential display |
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