Formation of multilayer aggregates of hepatocytes cultured on ligand-coated surfaces
Hepatocytes grown on various ligand (hepatocyte growth factor, HGF; low-density lipoprotein, LDL; transferrin; poly-N-p-vinylbenzyl-d-lactonamide, PVLA)-coated surfaces tended to keep round cell shapes and form multilayer aggregates in the presence of hepatocyte growth factor. Inhibitors of prolifer...
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| Veröffentlicht in: | Biotechnology letters 1999-12, Vol.21 (12), p.1065-1069 |
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| container_title | Biotechnology letters |
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| creator | JUN FENG LIANG AKAIKE, T |
| description | Hepatocytes grown on various ligand (hepatocyte growth factor, HGF; low-density lipoprotein, LDL; transferrin; poly-N-p-vinylbenzyl-d-lactonamide, PVLA)-coated surfaces tended to keep round cell shapes and form multilayer aggregates in the presence of hepatocyte growth factor. Inhibitors of proliferating signal transduction did not affect mitogen-induced hepatocyte aggregation, whereas colchicine, an antimicrotubular drug, completely inhibited the hepatocyte aggregation. Meanwhile, significant ligand loss was found on hepatocytes moving over surfaces and thus receptor-mediated endocytosis might be directly involved in the hepatocyte aggregation on ligand-coated surfaces.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1023/A:1005648913238 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 1999-12, Vol.21 (12), p.1065-1069 |
| issn | 0141-5492 1573-6776 |
| language | eng |
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| subjects | Aggregates Animal cells Biological and medical sciences Biotechnology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology hepatocyte growth factor Methods. Procedures. Technologies Miscellaneous poly-N-p-vinylbenzyl-D-lactonamide Proteins Signal transduction |
| title | Formation of multilayer aggregates of hepatocytes cultured on ligand-coated surfaces |
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