In Vitro Synthesis of Hyaluronan by a Single Protein Derived from Mouse HAS1 Gene and Characterization of Amino Acid Residues Essential for the Activity

HAS1 was expressed as a FLAG-tagged HAS1 fusion protein in COS-1 cells. This recombinant protein was extracted with CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid) from the membrane fraction and purified by anti-FLAG affinity chromatography and subsequent SDS-polyacrylamide gel...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 2000-01, Vol.275 (1), p.497-506
Hauptverfasser:	Yoshida, M, Itano, N, Yamada, Y, Kimata, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Motifs Amino Acid Sequence Animals Cell-Free System Chitin - metabolism Glucosamine - analogs & derivatives Glucosamine - metabolism Glucuronosyltransferase - genetics Glucuronosyltransferase - isolation & purification Glucuronosyltransferase - metabolism Glycosyltransferases HAS1 gene HAS1 protein Hyaluronan Hyaluronan Synthases Hyaluronic Acid - biosynthesis Membrane Proteins Membranes - metabolism Mice Molecular Sequence Data Oligosaccharides - metabolism Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Transferases Uridine Diphosphate N-Acetylglucosamine - metabolism Xenopus Proteins
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!