Isolation of ice-nucleating active bacterium from mackerel [Scomber australasicus] and its properties

"An ice-nucleating bacterium, designated MACK-4, was isolated from ice-stored mackerel (Scomber australasicus) and identified as a Pseudomonas fluorescens. The optimal temperature and pH for its growth in nutrient broth with 2.5% glycerol (NB-G) were 15 deg C and 6.5, respectively.The maximal i...

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Veröffentlicht in:	Fisheries science 2002-08, Vol.68 (4), p.934-941
Hauptverfasser:	"Chen, M.L. (National Taiwan Ocean Univ., Keelung), Chiou, T.K, Jiang, S.T.
Format:	Artikel
Sprache:	eng
Schlagworte:	ICE NUCLEATING BACTERIA ice‐nucleating activity (INA) ISOLATION MICROBIAL PROPERTIES number of ice nuclei INA/mL SCOMBER Scomber australasicus Seudomonas fluorescens
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creator	"Chen, M.L. (National Taiwan Ocean Univ., Keelung) Chiou, T.K Jiang, S.T.
description	"An ice-nucleating bacterium, designated MACK-4, was isolated from ice-stored mackerel (Scomber australasicus) and identified as a Pseudomonas fluorescens. The optimal temperature and pH for its growth in nutrient broth with 2.5% glycerol (NB-G) were 15 deg C and 6.5, respectively.The maximal ice-nucleating activity (INA) was obtained after 54 h incubation at 15 deg C. However, the INA was almost completely lost after 48 h incubation at 25 deg C or higher. The growth and INA decreased with increase of NaCI added in NB-G within 0.0-4.0%. The INA of MACK-4 was very stable at 5-25 deg C,pH 4.0-9.5, while that of isolated ice-nucleating matter from MACK-4 was stable at 5-25 deg C, pH 5.5-9.0."
doi_str_mv	10.1046/j.1444-2906.2002.00513.x
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(National Taiwan Ocean Univ., Keelung)</au><au>Chiou, T.K</au><au>Jiang, S.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of ice-nucleating active bacterium from mackerel [Scomber australasicus] and its properties</atitle><jtitle>Fisheries science</jtitle><date>2002-08</date><risdate>2002</risdate><volume>68</volume><issue>4</issue><spage>934</spage><epage>941</epage><pages>934-941</pages><issn>0919-9268</issn><eissn>1444-2906</eissn><abstract>"An ice-nucleating bacterium, designated MACK-4, was isolated from ice-stored mackerel (Scomber australasicus) and identified as a Pseudomonas fluorescens. The optimal temperature and pH for its growth in nutrient broth with 2.5% glycerol (NB-G) were 15 deg C and 6.5, respectively.The maximal ice-nucleating activity (INA) was obtained after 54 h incubation at 15 deg C. However, the INA was almost completely lost after 48 h incubation at 25 deg C or higher. The growth and INA decreased with increase of NaCI added in NB-G within 0.0-4.0%. The INA of MACK-4 was very stable at 5-25 deg C,pH 4.0-9.5, while that of isolated ice-nucleating matter from MACK-4 was stable at 5-25 deg C, pH 5.5-9.0."</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><doi>10.1046/j.1444-2906.2002.00513.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects	ICE NUCLEATING BACTERIA ice‐nucleating activity (INA) ISOLATION MICROBIAL PROPERTIES number of ice nuclei INA/mL SCOMBER Scomber australasicus Seudomonas fluorescens
title	Isolation of ice-nucleating active bacterium from mackerel [Scomber australasicus] and its properties
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