Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)
The efficient recovery of plants from avocado somatic embryos has been difficult to achieve by manipulating maturation and conversion conditions in vitro. The production of morphologically normal, bipolar somatic embryos occurs at low frequency, usually
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2005-07, Vol.82 (1), p.1-9 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Raharjo, S.H.T Litz, R.E |
| description | The efficient recovery of plants from avocado somatic embryos has been difficult to achieve by manipulating maturation and conversion conditions in vitro. The production of morphologically normal, bipolar somatic embryos occurs at low frequency, usually |
| doi_str_mv | 10.1007/s11240-004-5486-3 |
| format | Article |
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The production of morphologically normal, bipolar somatic embryos occurs at low frequency, usually <2%, and conversion is sporadic. In order to utilize the embryogenic system for improving avocado by genetic transformation and in vitro mutagenesis, different protocols for rescuing the shoots that develop from somatic embryos were evaluated. Shoots derived either from somatic embryos or from shoot tip and nodal cultures were either micrografted or grafted ex vitro onto seedling rootstocks. Depending on scion type, micrografting was 59-100% successful. For ex vitro grafting, several genotypes derived from shoot tip/nodal cultures were used as scions with either top slit or side grafting. Plant recovery after ex vitro grafting was 52-76%, and flushing generally occurred 20-25 days after grafting. Top slit and side grafting were equally successful (68-72%); however, the former resulted in earlier flushing (21 versus 29 days). These grafting procedures have been adopted for routine rescue of avocado regenerants from somatic embryos.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-004-5486-3</identifier><identifier>CODEN: PTCEDJ</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>avocados ; benzyladenine ; Biological and medical sciences ; Biotechnology ; culture media ; dose response ; embryo culture ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; gibberellic acid ; grafting (plants) ; in vitro culture ; in vitro regeneration ; methodology ; Methods. Procedures. Technologies ; micropropagation ; Miscellaneous ; Murashige and Skoog medium ; Persea americana ; Plant cells and fungal cells ; somatic embryos</subject><ispartof>Plant cell, tissue and organ culture, 2005-07, Vol.82 (1), p.1-9</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-fc1ac54e37e38ccb0524d7174e21f29ae2689771ee7cf661d5f3608600f18a593</citedby><cites>FETCH-LOGICAL-c396t-fc1ac54e37e38ccb0524d7174e21f29ae2689771ee7cf661d5f3608600f18a593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16741771$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Raharjo, S.H.T</creatorcontrib><creatorcontrib>Litz, R.E</creatorcontrib><title>Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)</title><title>Plant cell, tissue and organ culture</title><description>The efficient recovery of plants from avocado somatic embryos has been difficult to achieve by manipulating maturation and conversion conditions in vitro. The production of morphologically normal, bipolar somatic embryos occurs at low frequency, usually <2%, and conversion is sporadic. In order to utilize the embryogenic system for improving avocado by genetic transformation and in vitro mutagenesis, different protocols for rescuing the shoots that develop from somatic embryos were evaluated. Shoots derived either from somatic embryos or from shoot tip and nodal cultures were either micrografted or grafted ex vitro onto seedling rootstocks. Depending on scion type, micrografting was 59-100% successful. For ex vitro grafting, several genotypes derived from shoot tip/nodal cultures were used as scions with either top slit or side grafting. Plant recovery after ex vitro grafting was 52-76%, and flushing generally occurred 20-25 days after grafting. Top slit and side grafting were equally successful (68-72%); however, the former resulted in earlier flushing (21 versus 29 days). These grafting procedures have been adopted for routine rescue of avocado regenerants from somatic embryos.</description><subject>avocados</subject><subject>benzyladenine</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>culture media</subject><subject>dose response</subject><subject>embryo culture</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gibberellic acid</subject><subject>grafting (plants)</subject><subject>in vitro culture</subject><subject>in vitro regeneration</subject><subject>methodology</subject><subject>Methods. Procedures. Technologies</subject><subject>micropropagation</subject><subject>Miscellaneous</subject><subject>Murashige and Skoog medium</subject><subject>Persea americana</subject><subject>Plant cells and fungal cells</subject><subject>somatic embryos</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpFkU1vEzEQhi0EEqHwAzjhC4getp1Zf22OqCoUqRWVoGdr6owjo-w62JuI_HscUsFppFfPvBo9I8RbhAsEcJcVsdfQAejO6MF26plYoHGqM6D1c7EAtK6zg3EvxatafwKAVRoXYrpLoeR1oTinaS1pWkn-LfdpLln-S2MusuaR5hQkj4_lkGXhGnb8l99uaJpbEPKey0GmSdI-B1pl-fGeS2WSNHJJgSY6fy1eRNpUfvM0z8TD5-sfVzfd7bcvX68-3XZBLe3cxYAUjGblWA0hPILp9cqh09xj7JfEvR2WziGzC9FaXJmoLAwWIOJAZqnOxIdT77bkXzuusx9TDbxpp3LeVd-qDKBRDcQT2CzUWjj6bUkjlYNH8Eez_mTWN7P-aNYfd94_lVMNtImFppDq_0XrNLbjGvfuxEXKntalMQ_fe0AF2N5h7aD-AErmgpw</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Raharjo, S.H.T</creator><creator>Litz, R.E</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050701</creationdate><title>Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)</title><author>Raharjo, S.H.T ; Litz, R.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-fc1ac54e37e38ccb0524d7174e21f29ae2689771ee7cf661d5f3608600f18a593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>avocados</topic><topic>benzyladenine</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>culture media</topic><topic>dose response</topic><topic>embryo culture</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gibberellic acid</topic><topic>grafting (plants)</topic><topic>in vitro culture</topic><topic>in vitro regeneration</topic><topic>methodology</topic><topic>Methods. Procedures. Technologies</topic><topic>micropropagation</topic><topic>Miscellaneous</topic><topic>Murashige and Skoog medium</topic><topic>Persea americana</topic><topic>Plant cells and fungal cells</topic><topic>somatic embryos</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raharjo, S.H.T</creatorcontrib><creatorcontrib>Litz, R.E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raharjo, S.H.T</au><au>Litz, R.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana)</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><date>2005-07-01</date><risdate>2005</risdate><volume>82</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>The efficient recovery of plants from avocado somatic embryos has been difficult to achieve by manipulating maturation and conversion conditions in vitro. The production of morphologically normal, bipolar somatic embryos occurs at low frequency, usually <2%, and conversion is sporadic. In order to utilize the embryogenic system for improving avocado by genetic transformation and in vitro mutagenesis, different protocols for rescuing the shoots that develop from somatic embryos were evaluated. Shoots derived either from somatic embryos or from shoot tip and nodal cultures were either micrografted or grafted ex vitro onto seedling rootstocks. Depending on scion type, micrografting was 59-100% successful. For ex vitro grafting, several genotypes derived from shoot tip/nodal cultures were used as scions with either top slit or side grafting. Plant recovery after ex vitro grafting was 52-76%, and flushing generally occurred 20-25 days after grafting. Top slit and side grafting were equally successful (68-72%); however, the former resulted in earlier flushing (21 versus 29 days). These grafting procedures have been adopted for routine rescue of avocado regenerants from somatic embryos.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/s11240-004-5486-3</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0167-6857 |
| ispartof | Plant cell, tissue and organ culture, 2005-07, Vol.82 (1), p.1-9 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | avocados benzyladenine Biological and medical sciences Biotechnology culture media dose response embryo culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology gibberellic acid grafting (plants) in vitro culture in vitro regeneration methodology Methods. Procedures. Technologies micropropagation Miscellaneous Murashige and Skoog medium Persea americana Plant cells and fungal cells somatic embryos |
| title | Micrografting and ex vitro grafting for somatic embryo rescue and plant recovery in avocado (Persea americana) |
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