Capturing hepatocellular carcinoma cells using lactobionic acid-functionalized electrospun polyvinyl alcohol/polyethyleneimine nanofibers
We report a facile approach to immobilizing lactobionic acid (LA) onto electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers through a polyethylene glycol (PEG) spacer for capturing hepatocellular carcinoma cells. In this work, electrospun PVA/PEI nanofibers were crosslinked using g...
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| Veröffentlicht in: | RSC advances 2015-01, Vol.5 (86), p.70439-70447 |
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| container_title | RSC advances |
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| creator | Zhao, Yili Fan, Zhangyu Shen, Mingwu Shi, Xiangyang |
| description | We report a facile approach to immobilizing lactobionic acid (LA) onto electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers through a polyethylene glycol (PEG) spacer for capturing hepatocellular carcinoma cells. In this work, electrospun PVA/PEI nanofibers were crosslinked using glutaraldehyde vapor, covalently conjugated with PEGylated LA
via
an
N
-(3-dimethylaminopropyl)-
N
′-ethylcarbodiimide hydrochloride (EDC) coupling reaction, followed by acetylation of the remaining PEI amines on the fiber surface. The formed LA-functionalized nanofibers were characterized
via
scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. We show that the fiber morphology does not significantly change after fiber surface modification. The functionalized nanofibers display good hemocompatibility and superior capability to capture asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma cells
in vitro via
a ligand–receptor interaction. The developed LA-modified PVA/PEI nanofibers may be applied to capture circulating tumor cells for cancer diagnosis applications. |
| doi_str_mv | 10.1039/C5RA11662G |
| format | Article |
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via
an
N
-(3-dimethylaminopropyl)-
N
′-ethylcarbodiimide hydrochloride (EDC) coupling reaction, followed by acetylation of the remaining PEI amines on the fiber surface. The formed LA-functionalized nanofibers were characterized
via
scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. We show that the fiber morphology does not significantly change after fiber surface modification. The functionalized nanofibers display good hemocompatibility and superior capability to capture asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma cells
in vitro via
a ligand–receptor interaction. The developed LA-modified PVA/PEI nanofibers may be applied to capture circulating tumor cells for cancer diagnosis applications.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/C5RA11662G</identifier><language>eng</language><subject>Circulating ; Electrospinning ; Fibers ; Nanofibers ; Polyetherimides ; Polyethylene glycol ; Polyethyleneimine ; Polyvinyl alcohols</subject><ispartof>RSC advances, 2015-01, Vol.5 (86), p.70439-70447</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-616e0a0d51f5d079e7eafb4452a7f431c90c33ec07390d1eace123481e583bd33</citedby><cites>FETCH-LOGICAL-c330t-616e0a0d51f5d079e7eafb4452a7f431c90c33ec07390d1eace123481e583bd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Zhao, Yili</creatorcontrib><creatorcontrib>Fan, Zhangyu</creatorcontrib><creatorcontrib>Shen, Mingwu</creatorcontrib><creatorcontrib>Shi, Xiangyang</creatorcontrib><title>Capturing hepatocellular carcinoma cells using lactobionic acid-functionalized electrospun polyvinyl alcohol/polyethyleneimine nanofibers</title><title>RSC advances</title><description>We report a facile approach to immobilizing lactobionic acid (LA) onto electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers through a polyethylene glycol (PEG) spacer for capturing hepatocellular carcinoma cells. In this work, electrospun PVA/PEI nanofibers were crosslinked using glutaraldehyde vapor, covalently conjugated with PEGylated LA
via
an
N
-(3-dimethylaminopropyl)-
N
′-ethylcarbodiimide hydrochloride (EDC) coupling reaction, followed by acetylation of the remaining PEI amines on the fiber surface. The formed LA-functionalized nanofibers were characterized
via
scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. We show that the fiber morphology does not significantly change after fiber surface modification. The functionalized nanofibers display good hemocompatibility and superior capability to capture asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma cells
in vitro via
a ligand–receptor interaction. The developed LA-modified PVA/PEI nanofibers may be applied to capture circulating tumor cells for cancer diagnosis applications.</description><subject>Circulating</subject><subject>Electrospinning</subject><subject>Fibers</subject><subject>Nanofibers</subject><subject>Polyetherimides</subject><subject>Polyethylene glycol</subject><subject>Polyethyleneimine</subject><subject>Polyvinyl alcohols</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpNkEFLxDAQhYMouKx78RfkKELdpGnT7XFZdBUEQfRcpunUjaRJTVKh_gP_tS0r6Fxm3uNj4D1CLjm74UyU613-vOVcynR_QhYpy2SSMlme_rvPySqEdzaNzHkq-YJ876CPg9f2jR6wh-gUGjMY8FSBV9q6DuhsBTqEGTKgoqu1s1pRULpJ2sGqOGkw-gsbigZV9C70g6W9M-OntqOhYJQ7OLOeHYyH0aBF3WmL1IJ1ra7Rhwty1oIJuPrdS_J6d_uyu08en_YPu-1jooRgMZFcIgPW5LzNG1aUWCC0dZblKRRtJrgq2QSiYoUoWcMRFPJUZBuO-UbUjRBLcnX823v3MWCIVafDHBEsuiFUvMiyIuW5ZBN6fUTVlCh4bKve6w78WHFWzZVXf5WLH-sweBs</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Zhao, Yili</creator><creator>Fan, Zhangyu</creator><creator>Shen, Mingwu</creator><creator>Shi, Xiangyang</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150101</creationdate><title>Capturing hepatocellular carcinoma cells using lactobionic acid-functionalized electrospun polyvinyl alcohol/polyethyleneimine nanofibers</title><author>Zhao, Yili ; Fan, Zhangyu ; Shen, Mingwu ; Shi, Xiangyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-616e0a0d51f5d079e7eafb4452a7f431c90c33ec07390d1eace123481e583bd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Circulating</topic><topic>Electrospinning</topic><topic>Fibers</topic><topic>Nanofibers</topic><topic>Polyetherimides</topic><topic>Polyethylene glycol</topic><topic>Polyethyleneimine</topic><topic>Polyvinyl alcohols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yili</creatorcontrib><creatorcontrib>Fan, Zhangyu</creatorcontrib><creatorcontrib>Shen, Mingwu</creatorcontrib><creatorcontrib>Shi, Xiangyang</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yili</au><au>Fan, Zhangyu</au><au>Shen, Mingwu</au><au>Shi, Xiangyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capturing hepatocellular carcinoma cells using lactobionic acid-functionalized electrospun polyvinyl alcohol/polyethyleneimine nanofibers</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>86</issue><spage>70439</spage><epage>70447</epage><pages>70439-70447</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>We report a facile approach to immobilizing lactobionic acid (LA) onto electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers through a polyethylene glycol (PEG) spacer for capturing hepatocellular carcinoma cells. In this work, electrospun PVA/PEI nanofibers were crosslinked using glutaraldehyde vapor, covalently conjugated with PEGylated LA
via
an
N
-(3-dimethylaminopropyl)-
N
′-ethylcarbodiimide hydrochloride (EDC) coupling reaction, followed by acetylation of the remaining PEI amines on the fiber surface. The formed LA-functionalized nanofibers were characterized
via
scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy. We show that the fiber morphology does not significantly change after fiber surface modification. The functionalized nanofibers display good hemocompatibility and superior capability to capture asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma cells
in vitro via
a ligand–receptor interaction. The developed LA-modified PVA/PEI nanofibers may be applied to capture circulating tumor cells for cancer diagnosis applications.</abstract><doi>10.1039/C5RA11662G</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2046-2069 |
| ispartof | RSC advances, 2015-01, Vol.5 (86), p.70439-70447 |
| issn | 2046-2069 2046-2069 |
| language | eng |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Circulating Electrospinning Fibers Nanofibers Polyetherimides Polyethylene glycol Polyethyleneimine Polyvinyl alcohols |
| title | Capturing hepatocellular carcinoma cells using lactobionic acid-functionalized electrospun polyvinyl alcohol/polyethyleneimine nanofibers |
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