Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry
Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether th...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2012-06, Vol.403 (7), p.1863-1871 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1871 |
|---|---|
| container_issue | 7 |
| container_start_page | 1863 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 403 |
| creator | Goto-Inoue, Naoko Manabe, Yasuko Miyatake, Shouta Ogino, Shinya Morishita, Ai Hayasaka, Takahiro Masaki, Noritaka Setou, Mitsutoshi Fujii, Nobuharu L. |
| description | Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether the lipid composition of skeletal muscle changes by contraction, due to the complexity of lipid molecular species. In this study, we used matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to investigate changes in skeletal muscle lipid composition induced by contraction. We successfully observed the reduction of diacylglycerol and triacylglycerol, which are generally associated with muscle contraction. Interestingly, we found the accumulation of some saturated and mono-unsaturated fatty acids and poly-unsaturated fatty acids containing phosphatidylcholine in contracted muscles. Moreover, the distributions of several types of lipid were changed by contraction. Our results show that changes in the lipid amount, lipid composition, and energy metabolic activity can be evaluated in each local spot of cells and tissues at the same time using MALDI-IMS. In conclusion, MALDI-IMS is a powerful tool for studying lipid changes associated with contractions. |
| doi_str_mv | 10.1007/s00216-012-5809-x |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1744721016</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A429779741</galeid><sourcerecordid>A429779741</sourcerecordid><originalsourceid>FETCH-LOGICAL-c609t-2564dbe66454c56763375c8c3b7b670657b093339b5b634f072a855cdbf36d8f3</originalsourceid><addsrcrecordid>eNqNkl9r1jAUxosobk4_gDcS8Mabbvmf9HIMncLAG_U2pGn6mtkmNaeF9_Uj-SlN120giBshJCS_5-SE56mq1wSfEozVGWBMiawxobXQuKn3T6pjIomuqRT46f2e06PqBcA1xkRoIp9XR5Qy3jBOj6vf3wIsdgi_7BxSRKlH3SHaMTjkvtu48yhE5FKcs3UrUIfYLc53aAhT6MrNOCUIN9ICjmkBj-CHH_xsBzQu4AaP2gMa7ZzDvrYAAeZVbcFn1HlIeVrFZ2XetRBGuwtxVzQACCbv5pxGP-fDy-pZbwfwr27Xk-rrh_dfLj7WV58vP12cX9VO4mauqZC8a72UXHAnpJKMKeG0Y61qpcJSqBY3jLGmFa1kvMeKWi2E69qeyU737KR6t9Wdcvq5eJjNGMD5YbDRlw8aojhXlGAiH4dirTB7GKWU6FKW8YdRrAuspH5EA8VyJbjSTUHfbujODt6E2KfV1BU355w2SjWKk0Kd_oMqo_MlFCn6PpTzvwRkE7icALLvzZSLhflQ3jZrSs2WUlNSataUmn3RvLntemlH390r7mJZALoBUK5KCrO5TkuOxfX_VP0Dghzz0g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1015754789</pqid></control><display><type>article</type><title>Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Goto-Inoue, Naoko ; Manabe, Yasuko ; Miyatake, Shouta ; Ogino, Shinya ; Morishita, Ai ; Hayasaka, Takahiro ; Masaki, Noritaka ; Setou, Mitsutoshi ; Fujii, Nobuharu L.</creator><creatorcontrib>Goto-Inoue, Naoko ; Manabe, Yasuko ; Miyatake, Shouta ; Ogino, Shinya ; Morishita, Ai ; Hayasaka, Takahiro ; Masaki, Noritaka ; Setou, Mitsutoshi ; Fujii, Nobuharu L.</creatorcontrib><description>Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether the lipid composition of skeletal muscle changes by contraction, due to the complexity of lipid molecular species. In this study, we used matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to investigate changes in skeletal muscle lipid composition induced by contraction. We successfully observed the reduction of diacylglycerol and triacylglycerol, which are generally associated with muscle contraction. Interestingly, we found the accumulation of some saturated and mono-unsaturated fatty acids and poly-unsaturated fatty acids containing phosphatidylcholine in contracted muscles. Moreover, the distributions of several types of lipid were changed by contraction. Our results show that changes in the lipid amount, lipid composition, and energy metabolic activity can be evaluated in each local spot of cells and tissues at the same time using MALDI-IMS. In conclusion, MALDI-IMS is a powerful tool for studying lipid changes associated with contractions.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-012-5809-x</identifier><identifier>PMID: 22349342</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analytical Chemistry ; Animals ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Desorption ; Fatty acids ; Food Science ; Imaging ; Insulin resistance ; Ionization ; Laboratory Medicine ; Lasers ; Lipids ; Lipids - analysis ; Lipids - chemistry ; Male ; Mass spectrometry ; Mice ; Mice, Inbred ICR ; Monitoring/Environmental Analysis ; Muscle, Skeletal - chemistry ; Muscles ; Obesity ; Original Paper ; Physiological aspects ; Skeletal muscle ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Tandem Mass Spectrometry ; Triglycerides ; Unsaturated fatty acids</subject><ispartof>Analytical and bioanalytical chemistry, 2012-06, Vol.403 (7), p.1863-1871</ispartof><rights>Springer-Verlag 2012</rights><rights>COPYRIGHT 2012 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c609t-2564dbe66454c56763375c8c3b7b670657b093339b5b634f072a855cdbf36d8f3</citedby><cites>FETCH-LOGICAL-c609t-2564dbe66454c56763375c8c3b7b670657b093339b5b634f072a855cdbf36d8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-012-5809-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-012-5809-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22349342$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goto-Inoue, Naoko</creatorcontrib><creatorcontrib>Manabe, Yasuko</creatorcontrib><creatorcontrib>Miyatake, Shouta</creatorcontrib><creatorcontrib>Ogino, Shinya</creatorcontrib><creatorcontrib>Morishita, Ai</creatorcontrib><creatorcontrib>Hayasaka, Takahiro</creatorcontrib><creatorcontrib>Masaki, Noritaka</creatorcontrib><creatorcontrib>Setou, Mitsutoshi</creatorcontrib><creatorcontrib>Fujii, Nobuharu L.</creatorcontrib><title>Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether the lipid composition of skeletal muscle changes by contraction, due to the complexity of lipid molecular species. In this study, we used matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to investigate changes in skeletal muscle lipid composition induced by contraction. We successfully observed the reduction of diacylglycerol and triacylglycerol, which are generally associated with muscle contraction. Interestingly, we found the accumulation of some saturated and mono-unsaturated fatty acids and poly-unsaturated fatty acids containing phosphatidylcholine in contracted muscles. Moreover, the distributions of several types of lipid were changed by contraction. Our results show that changes in the lipid amount, lipid composition, and energy metabolic activity can be evaluated in each local spot of cells and tissues at the same time using MALDI-IMS. In conclusion, MALDI-IMS is a powerful tool for studying lipid changes associated with contractions.</description><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Desorption</subject><subject>Fatty acids</subject><subject>Food Science</subject><subject>Imaging</subject><subject>Insulin resistance</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Lasers</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Lipids - chemistry</subject><subject>Male</subject><subject>Mass spectrometry</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Monitoring/Environmental Analysis</subject><subject>Muscle, Skeletal - chemistry</subject><subject>Muscles</subject><subject>Obesity</subject><subject>Original Paper</subject><subject>Physiological aspects</subject><subject>Skeletal muscle</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Tandem Mass Spectrometry</subject><subject>Triglycerides</subject><subject>Unsaturated fatty acids</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl9r1jAUxosobk4_gDcS8Mabbvmf9HIMncLAG_U2pGn6mtkmNaeF9_Uj-SlN120giBshJCS_5-SE56mq1wSfEozVGWBMiawxobXQuKn3T6pjIomuqRT46f2e06PqBcA1xkRoIp9XR5Qy3jBOj6vf3wIsdgi_7BxSRKlH3SHaMTjkvtu48yhE5FKcs3UrUIfYLc53aAhT6MrNOCUIN9ICjmkBj-CHH_xsBzQu4AaP2gMa7ZzDvrYAAeZVbcFn1HlIeVrFZ2XetRBGuwtxVzQACCbv5pxGP-fDy-pZbwfwr27Xk-rrh_dfLj7WV58vP12cX9VO4mauqZC8a72UXHAnpJKMKeG0Y61qpcJSqBY3jLGmFa1kvMeKWi2E69qeyU737KR6t9Wdcvq5eJjNGMD5YbDRlw8aojhXlGAiH4dirTB7GKWU6FKW8YdRrAuspH5EA8VyJbjSTUHfbujODt6E2KfV1BU355w2SjWKk0Kd_oMqo_MlFCn6PpTzvwRkE7icALLvzZSLhflQ3jZrSs2WUlNSataUmn3RvLntemlH390r7mJZALoBUK5KCrO5TkuOxfX_VP0Dghzz0g</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Goto-Inoue, Naoko</creator><creator>Manabe, Yasuko</creator><creator>Miyatake, Shouta</creator><creator>Ogino, Shinya</creator><creator>Morishita, Ai</creator><creator>Hayasaka, Takahiro</creator><creator>Masaki, Noritaka</creator><creator>Setou, Mitsutoshi</creator><creator>Fujii, Nobuharu L.</creator><general>Springer-Verlag</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope><scope>7SR</scope><scope>7SU</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20120601</creationdate><title>Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry</title><author>Goto-Inoue, Naoko ; Manabe, Yasuko ; Miyatake, Shouta ; Ogino, Shinya ; Morishita, Ai ; Hayasaka, Takahiro ; Masaki, Noritaka ; Setou, Mitsutoshi ; Fujii, Nobuharu L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c609t-2564dbe66454c56763375c8c3b7b670657b093339b5b634f072a855cdbf36d8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Desorption</topic><topic>Fatty acids</topic><topic>Food Science</topic><topic>Imaging</topic><topic>Insulin resistance</topic><topic>Ionization</topic><topic>Laboratory Medicine</topic><topic>Lasers</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Lipids - chemistry</topic><topic>Male</topic><topic>Mass spectrometry</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Monitoring/Environmental Analysis</topic><topic>Muscle, Skeletal - chemistry</topic><topic>Muscles</topic><topic>Obesity</topic><topic>Original Paper</topic><topic>Physiological aspects</topic><topic>Skeletal muscle</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Tandem Mass Spectrometry</topic><topic>Triglycerides</topic><topic>Unsaturated fatty acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goto-Inoue, Naoko</creatorcontrib><creatorcontrib>Manabe, Yasuko</creatorcontrib><creatorcontrib>Miyatake, Shouta</creatorcontrib><creatorcontrib>Ogino, Shinya</creatorcontrib><creatorcontrib>Morishita, Ai</creatorcontrib><creatorcontrib>Hayasaka, Takahiro</creatorcontrib><creatorcontrib>Masaki, Noritaka</creatorcontrib><creatorcontrib>Setou, Mitsutoshi</creatorcontrib><creatorcontrib>Fujii, Nobuharu L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineered Materials Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goto-Inoue, Naoko</au><au>Manabe, Yasuko</au><au>Miyatake, Shouta</au><au>Ogino, Shinya</au><au>Morishita, Ai</au><au>Hayasaka, Takahiro</au><au>Masaki, Noritaka</au><au>Setou, Mitsutoshi</au><au>Fujii, Nobuharu L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>403</volume><issue>7</issue><spage>1863</spage><epage>1871</epage><pages>1863-1871</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether the lipid composition of skeletal muscle changes by contraction, due to the complexity of lipid molecular species. In this study, we used matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to investigate changes in skeletal muscle lipid composition induced by contraction. We successfully observed the reduction of diacylglycerol and triacylglycerol, which are generally associated with muscle contraction. Interestingly, we found the accumulation of some saturated and mono-unsaturated fatty acids and poly-unsaturated fatty acids containing phosphatidylcholine in contracted muscles. Moreover, the distributions of several types of lipid were changed by contraction. Our results show that changes in the lipid amount, lipid composition, and energy metabolic activity can be evaluated in each local spot of cells and tissues at the same time using MALDI-IMS. In conclusion, MALDI-IMS is a powerful tool for studying lipid changes associated with contractions.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22349342</pmid><doi>10.1007/s00216-012-5809-x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2012-06, Vol.403 (7), p.1863-1871 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1744721016 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Analytical Chemistry Animals Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Desorption Fatty acids Food Science Imaging Insulin resistance Ionization Laboratory Medicine Lasers Lipids Lipids - analysis Lipids - chemistry Male Mass spectrometry Mice Mice, Inbred ICR Monitoring/Environmental Analysis Muscle, Skeletal - chemistry Muscles Obesity Original Paper Physiological aspects Skeletal muscle Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Tandem Mass Spectrometry Triglycerides Unsaturated fatty acids |
| title | Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T22%3A04%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Visualization%20of%20dynamic%20change%20in%20contraction-induced%20lipid%20composition%20in%20mouse%20skeletal%20muscle%20by%20matrix-assisted%20laser%20desorption/ionization%20imaging%20mass%20spectrometry&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Goto-Inoue,%20Naoko&rft.date=2012-06-01&rft.volume=403&rft.issue=7&rft.spage=1863&rft.epage=1871&rft.pages=1863-1871&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-012-5809-x&rft_dat=%3Cgale_proqu%3EA429779741%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1015754789&rft_id=info:pmid/22349342&rft_galeid=A429779741&rfr_iscdi=true |