A green method of staining DNA in polyacrylamide gel electrophoresis based on fluorescent copper nanoclusters synthesized in situ
The safety of nucleic acid staining dyes has long been recognized to be a problem. Extensive efforts have been made to search for alternatives to the most popular but toxic staining dye, ethidium bromide （EtBr）. However, so far no staining method that can be guaranteed to be suffidently safe has bee...
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| Veröffentlicht in: | Nano research 2015-08, Vol.8 (8), p.2714-2720 |
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| creator | Zhu, Xiaoli Shi, Hai Shen, Yalan Zhang, Bin Zhao, Jing Li, Genxi |
| description | The safety of nucleic acid staining dyes has long been recognized to be a problem. Extensive efforts have been made to search for alternatives to the most popular but toxic staining dye, ethidium bromide （EtBr）. However, so far no staining method that can be guaranteed to be suffidently safe has been developed. In this paper, we report a green staining method of DNA in polyacrylamide gel electrophoresis, where in situ synthesis of DNA-templated fluorescent copper nanoclusters （CuNCs） in the gel is achieved to make the DNA bands visible under UV light. Moreover, a comprehensive study of the performance of this staining method has been conducted and the experimental results show that it has favorable sensitivity, stability, and usability. Meanwhile, in our animal experiments, the two reagents （copper sulfate and ascorbic acid） as well as the synthesized CuNCs have been proven to be non-toxic in contact with skin. In addition, all the reagents employed in this work are readily available and low cost, and the procedure is simple to carry out. Therefore, this novel staining method based on the in situ synthesis DNA-templated fluorescent CuNCs has many potential applications. |
| doi_str_mv | 10.1007/s12274-015-0778-y |
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Extensive efforts have been made to search for alternatives to the most popular but toxic staining dye, ethidium bromide （EtBr）. However, so far no staining method that can be guaranteed to be suffidently safe has been developed. In this paper, we report a green staining method of DNA in polyacrylamide gel electrophoresis, where in situ synthesis of DNA-templated fluorescent copper nanoclusters （CuNCs） in the gel is achieved to make the DNA bands visible under UV light. Moreover, a comprehensive study of the performance of this staining method has been conducted and the experimental results show that it has favorable sensitivity, stability, and usability. Meanwhile, in our animal experiments, the two reagents （copper sulfate and ascorbic acid） as well as the synthesized CuNCs have been proven to be non-toxic in contact with skin. In addition, all the reagents employed in this work are readily available and low cost, and the procedure is simple to carry out. Therefore, this novel staining method based on the in situ synthesis DNA-templated fluorescent CuNCs has many potential applications.</description><identifier>ISSN: 1998-0124</identifier><identifier>EISSN: 1998-0000</identifier><identifier>DOI: 10.1007/s12274-015-0778-y</identifier><language>eng</language><publisher>Beijing: Tsinghua University Press</publisher><subject>Acids ; Atomic/Molecular Structure and Spectra ; Biochemistry ; Biomedicine ; Biotechnology ; Chemistry and Materials Science ; Condensed Matter Physics ; Copper ; COPPER SULFATE ; Deoxyribonucleic acid ; DNA ; DNA模板 ; Dyes ; Electrophoresis ; FABRICATION ; Fluorescence ; Materials Science ; MICROSTRUCTURES ; Nanomaterials ; Nanostructure ; Nanotechnology ; Nucleic acids ; Optimization ; Polyacrylamides ; Reagents ; Research Article ; Staining ; Synthesis ; Toxicity ; Ultraviolet radiation ; 动物实验 ; 原位合成 ; 染色方法 ; 硫酸铜 ; 纳米簇 ; 绿色方法 ; 聚丙烯酰胺凝胶电泳</subject><ispartof>Nano research, 2015-08, Vol.8 (8), p.2714-2720</ispartof><rights>Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-6fbddead97ba8d60cd71c48ada3665aa6a9bc2bd56d2f5caf03306ae130489ed3</citedby><cites>FETCH-LOGICAL-c479t-6fbddead97ba8d60cd71c48ada3665aa6a9bc2bd56d2f5caf03306ae130489ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71233X/71233X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12274-015-0778-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12274-015-0778-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Zhu, Xiaoli</creatorcontrib><creatorcontrib>Shi, Hai</creatorcontrib><creatorcontrib>Shen, Yalan</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><creatorcontrib>Zhao, Jing</creatorcontrib><creatorcontrib>Li, Genxi</creatorcontrib><title>A green method of staining DNA in polyacrylamide gel electrophoresis based on fluorescent copper nanoclusters synthesized in situ</title><title>Nano research</title><addtitle>Nano Res</addtitle><addtitle>Nano Research</addtitle><description>The safety of nucleic acid staining dyes has long been recognized to be a problem. 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Therefore, this novel staining method based on the in situ synthesis DNA-templated fluorescent CuNCs has many potential applications.</description><subject>Acids</subject><subject>Atomic/Molecular Structure and Spectra</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Chemistry and Materials Science</subject><subject>Condensed Matter Physics</subject><subject>Copper</subject><subject>COPPER SULFATE</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA模板</subject><subject>Dyes</subject><subject>Electrophoresis</subject><subject>FABRICATION</subject><subject>Fluorescence</subject><subject>Materials Science</subject><subject>MICROSTRUCTURES</subject><subject>Nanomaterials</subject><subject>Nanostructure</subject><subject>Nanotechnology</subject><subject>Nucleic acids</subject><subject>Optimization</subject><subject>Polyacrylamides</subject><subject>Reagents</subject><subject>Research 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Xiaoli</au><au>Shi, Hai</au><au>Shen, Yalan</au><au>Zhang, Bin</au><au>Zhao, Jing</au><au>Li, Genxi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A green method of staining DNA in polyacrylamide gel electrophoresis based on fluorescent copper nanoclusters synthesized in situ</atitle><jtitle>Nano research</jtitle><stitle>Nano Res</stitle><addtitle>Nano Research</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>8</volume><issue>8</issue><spage>2714</spage><epage>2720</epage><pages>2714-2720</pages><issn>1998-0124</issn><eissn>1998-0000</eissn><abstract>The safety of nucleic acid staining dyes has long been recognized to be a problem. Extensive efforts have been made to search for alternatives to the most popular but toxic staining dye, ethidium bromide （EtBr）. However, so far no staining method that can be guaranteed to be suffidently safe has been developed. In this paper, we report a green staining method of DNA in polyacrylamide gel electrophoresis, where in situ synthesis of DNA-templated fluorescent copper nanoclusters （CuNCs） in the gel is achieved to make the DNA bands visible under UV light. Moreover, a comprehensive study of the performance of this staining method has been conducted and the experimental results show that it has favorable sensitivity, stability, and usability. Meanwhile, in our animal experiments, the two reagents （copper sulfate and ascorbic acid） as well as the synthesized CuNCs have been proven to be non-toxic in contact with skin. In addition, all the reagents employed in this work are readily available and low cost, and the procedure is simple to carry out. Therefore, this novel staining method based on the in situ synthesis DNA-templated fluorescent CuNCs has many potential applications.</abstract><cop>Beijing</cop><pub>Tsinghua University Press</pub><doi>10.1007/s12274-015-0778-y</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 1998-0124 |
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| subjects | Acids Atomic/Molecular Structure and Spectra Biochemistry Biomedicine Biotechnology Chemistry and Materials Science Condensed Matter Physics Copper COPPER SULFATE Deoxyribonucleic acid DNA DNA模板 Dyes Electrophoresis FABRICATION Fluorescence Materials Science MICROSTRUCTURES Nanomaterials Nanostructure Nanotechnology Nucleic acids Optimization Polyacrylamides Reagents Research Article Staining Synthesis Toxicity Ultraviolet radiation 动物实验 原位合成 染色方法 硫酸铜 纳米簇 绿色方法 聚丙烯酰胺凝胶电泳 |
| title | A green method of staining DNA in polyacrylamide gel electrophoresis based on fluorescent copper nanoclusters synthesized in situ |
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