Integrins Direct Cell Adhesion in a Substrate-Dependent Manner
The relationship between substrate properties and cell behavior is complex, including roles for both mechanics and biochemistry. Here we investigate the role of viscous dissipation on cell adhesion behaviors, using polymer films of tunable lateral mobility. We find that fibroblasts selectively use α...
Gespeichert in:
| Veröffentlicht in: | Cellular and molecular bioengineering 2015-09, Vol.8 (3), p.488-495 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 495 |
|---|---|
| container_issue | 3 |
| container_start_page | 488 |
| container_title | Cellular and molecular bioengineering |
| container_volume | 8 |
| creator | Kourouklis, Andreas P. Bermudez, Harry |
| description | The relationship between substrate properties and cell behavior is complex, including roles for both mechanics and biochemistry. Here we investigate the role of viscous dissipation on cell adhesion behaviors, using polymer films of tunable lateral mobility. We find that fibroblasts selectively use
α
v
β
3
and
α
5
β
1
integrin receptors to control their spreading area and polarization on low and high mobility films, respectively. In addition, the dynamics of cell spreading and polarization are well described by a semi-empirical sigmoidal relationship. Analysis of cell dynamic behavior reveals that spreading dynamics are controlled by the availability of integrins, whereas the polarization dynamics are controlled by intracellular signaling. The result that cells preferentially use specific integrin receptors in response to substrate mechanical properties has broad implications for processes in dynamic environments such as wound healing and cancer metastasis. |
| doi_str_mv | 10.1007/s12195-015-0394-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1744684578</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3781271971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-945b59970222e6316c7485834df55006ecd4511021cb7bef9f12fa8b8d5923293</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMouK7-AG8FL16qmXw0yUVY1q-FFQ_qOaTtdO2ym65Je_Dfm6UiIngYZg7PO7w8hJwDvQJK1XUEBkbmFNJwI3J1QCagC5lLyvnhz83kMTmJcU1pwSgXE3Kz8D2uQutjdtsGrPpsjptNNqvfMbadz1qfuexlKGMfXI_5Le7Q1-j77Ml5j-GUHDVuE_Hse0_J2_3d6_wxXz4_LOazZV4JMH1uhCylMYoyxrDgUFRKaKm5qBspUxesaiEBKIOqVCU2pgHWOF3qWhrGmeFTcjn-3YXuY8DY220bq9TUeeyGaEEJUWghlU7oxR903Q3Bp3aJosJwwYRMFIxUFboYAzZ2F9qtC58WqN0btaNRm4zavVGrUoaNmZhYv8Lw6_O_oS9YX3Wx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1704934245</pqid></control><display><type>article</type><title>Integrins Direct Cell Adhesion in a Substrate-Dependent Manner</title><source>SpringerNature Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Kourouklis, Andreas P. ; Bermudez, Harry</creator><creatorcontrib>Kourouklis, Andreas P. ; Bermudez, Harry</creatorcontrib><description>The relationship between substrate properties and cell behavior is complex, including roles for both mechanics and biochemistry. Here we investigate the role of viscous dissipation on cell adhesion behaviors, using polymer films of tunable lateral mobility. We find that fibroblasts selectively use
α
v
β
3
and
α
5
β
1
integrin receptors to control their spreading area and polarization on low and high mobility films, respectively. In addition, the dynamics of cell spreading and polarization are well described by a semi-empirical sigmoidal relationship. Analysis of cell dynamic behavior reveals that spreading dynamics are controlled by the availability of integrins, whereas the polarization dynamics are controlled by intracellular signaling. The result that cells preferentially use specific integrin receptors in response to substrate mechanical properties has broad implications for processes in dynamic environments such as wound healing and cancer metastasis.</description><identifier>ISSN: 1865-5025</identifier><identifier>EISSN: 1865-5033</identifier><identifier>DOI: 10.1007/s12195-015-0394-7</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adhesion ; Biochemistry ; Bioengineering ; Biological and Medical Physics ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical Engineering/Biotechnology ; Biophysics ; Cell adhesion ; Cell adhesion & migration ; Cell Biology ; Cellular ; Copolymers ; Dynamic tests ; Dynamics ; Engineering ; Mechanical properties ; Polarization ; Polymers ; Receptors ; Spreading ; Substrates</subject><ispartof>Cellular and molecular bioengineering, 2015-09, Vol.8 (3), p.488-495</ispartof><rights>Biomedical Engineering Society 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-945b59970222e6316c7485834df55006ecd4511021cb7bef9f12fa8b8d5923293</citedby><cites>FETCH-LOGICAL-c419t-945b59970222e6316c7485834df55006ecd4511021cb7bef9f12fa8b8d5923293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12195-015-0394-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12195-015-0394-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids></links><search><creatorcontrib>Kourouklis, Andreas P.</creatorcontrib><creatorcontrib>Bermudez, Harry</creatorcontrib><title>Integrins Direct Cell Adhesion in a Substrate-Dependent Manner</title><title>Cellular and molecular bioengineering</title><addtitle>Cel. Mol. Bioeng</addtitle><description>The relationship between substrate properties and cell behavior is complex, including roles for both mechanics and biochemistry. Here we investigate the role of viscous dissipation on cell adhesion behaviors, using polymer films of tunable lateral mobility. We find that fibroblasts selectively use
α
v
β
3
and
α
5
β
1
integrin receptors to control their spreading area and polarization on low and high mobility films, respectively. In addition, the dynamics of cell spreading and polarization are well described by a semi-empirical sigmoidal relationship. Analysis of cell dynamic behavior reveals that spreading dynamics are controlled by the availability of integrins, whereas the polarization dynamics are controlled by intracellular signaling. The result that cells preferentially use specific integrin receptors in response to substrate mechanical properties has broad implications for processes in dynamic environments such as wound healing and cancer metastasis.</description><subject>Adhesion</subject><subject>Biochemistry</subject><subject>Bioengineering</subject><subject>Biological and Medical Physics</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biophysics</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Biology</subject><subject>Cellular</subject><subject>Copolymers</subject><subject>Dynamic tests</subject><subject>Dynamics</subject><subject>Engineering</subject><subject>Mechanical properties</subject><subject>Polarization</subject><subject>Polymers</subject><subject>Receptors</subject><subject>Spreading</subject><subject>Substrates</subject><issn>1865-5025</issn><issn>1865-5033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1LxDAQhoMouK7-AG8FL16qmXw0yUVY1q-FFQ_qOaTtdO2ym65Je_Dfm6UiIngYZg7PO7w8hJwDvQJK1XUEBkbmFNJwI3J1QCagC5lLyvnhz83kMTmJcU1pwSgXE3Kz8D2uQutjdtsGrPpsjptNNqvfMbadz1qfuexlKGMfXI_5Le7Q1-j77Ml5j-GUHDVuE_Hse0_J2_3d6_wxXz4_LOazZV4JMH1uhCylMYoyxrDgUFRKaKm5qBspUxesaiEBKIOqVCU2pgHWOF3qWhrGmeFTcjn-3YXuY8DY220bq9TUeeyGaEEJUWghlU7oxR903Q3Bp3aJosJwwYRMFIxUFboYAzZ2F9qtC58WqN0btaNRm4zavVGrUoaNmZhYv8Lw6_O_oS9YX3Wx</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Kourouklis, Andreas P.</creator><creator>Bermudez, Harry</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20150901</creationdate><title>Integrins Direct Cell Adhesion in a Substrate-Dependent Manner</title><author>Kourouklis, Andreas P. ; Bermudez, Harry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-945b59970222e6316c7485834df55006ecd4511021cb7bef9f12fa8b8d5923293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adhesion</topic><topic>Biochemistry</topic><topic>Bioengineering</topic><topic>Biological and Medical Physics</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biophysics</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell Biology</topic><topic>Cellular</topic><topic>Copolymers</topic><topic>Dynamic tests</topic><topic>Dynamics</topic><topic>Engineering</topic><topic>Mechanical properties</topic><topic>Polarization</topic><topic>Polymers</topic><topic>Receptors</topic><topic>Spreading</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kourouklis, Andreas P.</creatorcontrib><creatorcontrib>Bermudez, Harry</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Cellular and molecular bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kourouklis, Andreas P.</au><au>Bermudez, Harry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrins Direct Cell Adhesion in a Substrate-Dependent Manner</atitle><jtitle>Cellular and molecular bioengineering</jtitle><stitle>Cel. Mol. Bioeng</stitle><date>2015-09-01</date><risdate>2015</risdate><volume>8</volume><issue>3</issue><spage>488</spage><epage>495</epage><pages>488-495</pages><issn>1865-5025</issn><eissn>1865-5033</eissn><abstract>The relationship between substrate properties and cell behavior is complex, including roles for both mechanics and biochemistry. Here we investigate the role of viscous dissipation on cell adhesion behaviors, using polymer films of tunable lateral mobility. We find that fibroblasts selectively use
α
v
β
3
and
α
5
β
1
integrin receptors to control their spreading area and polarization on low and high mobility films, respectively. In addition, the dynamics of cell spreading and polarization are well described by a semi-empirical sigmoidal relationship. Analysis of cell dynamic behavior reveals that spreading dynamics are controlled by the availability of integrins, whereas the polarization dynamics are controlled by intracellular signaling. The result that cells preferentially use specific integrin receptors in response to substrate mechanical properties has broad implications for processes in dynamic environments such as wound healing and cancer metastasis.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12195-015-0394-7</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1865-5025 |
| ispartof | Cellular and molecular bioengineering, 2015-09, Vol.8 (3), p.488-495 |
| issn | 1865-5025 1865-5033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1744684578 |
| source | SpringerNature Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adhesion Biochemistry Bioengineering Biological and Medical Physics Biomaterials Biomedical Engineering and Bioengineering Biomedical Engineering/Biotechnology Biophysics Cell adhesion Cell adhesion & migration Cell Biology Cellular Copolymers Dynamic tests Dynamics Engineering Mechanical properties Polarization Polymers Receptors Spreading Substrates |
| title | Integrins Direct Cell Adhesion in a Substrate-Dependent Manner |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T14%3A30%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Integrins%20Direct%20Cell%20Adhesion%20in%20a%20Substrate-Dependent%20Manner&rft.jtitle=Cellular%20and%20molecular%20bioengineering&rft.au=Kourouklis,%20Andreas%20P.&rft.date=2015-09-01&rft.volume=8&rft.issue=3&rft.spage=488&rft.epage=495&rft.pages=488-495&rft.issn=1865-5025&rft.eissn=1865-5033&rft_id=info:doi/10.1007/s12195-015-0394-7&rft_dat=%3Cproquest_cross%3E3781271971%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1704934245&rft_id=info:pmid/&rfr_iscdi=true |