Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging
We synthesized and characterized gold nanostars and their silica-coated derivatives with 7- to 50-nm shell thicknesses as contrast agents for optical imaging. The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflect...
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| Veröffentlicht in: | Journal of biomedical optics 2015-07, Vol.20 (7), p.076017-076017 |
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| container_title | Journal of biomedical optics |
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| creator | Bibikova, Olga Popov, Alexey Bykov, Alexander Prilepskii, Artur Kinnunen, Matti Kordas, Krisztian Bogatyrev, Vladimir Khlebtsov, Nikolai Vainio, Seppo Tuchin, Valery |
| description | We synthesized and characterized gold nanostars and their silica-coated derivatives with 7- to 50-nm shell thicknesses as contrast agents for optical imaging. The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflectance/transmittance analyses. The contrasting properties of the nanostructures were studied in optical coherence tomography glass capillary imaging. The silica-coated nanostars with the thickest shell have higher scattering ability in comparison with bare nanostars. Viability assays confirmed weak in vitro toxicity of nanostructures at up to ∼200-μg/mL concentrations. We showed real-time visualization of nanostars in both agarose and cultured cells by analyzing the backscattering signal using a conventional laser confocal microscope. The signal intensity detected from the silica-coated NPs was almost 1.5 times higher in comparison with bare nanostars. To the best of our knowledge, this is the first time that conventional laser confocal microscopy was applied in combined scattering and transmitted light modes to detect the backscattered signal of gold nanostars, which is useful for direct monitoring of the uptake, translocation, and accumulation of NPs in living cells. |
| doi_str_mv | 10.1117/1.JBO.20.7.076017 |
| format | Article |
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The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflectance/transmittance analyses. The contrasting properties of the nanostructures were studied in optical coherence tomography glass capillary imaging. The silica-coated nanostars with the thickest shell have higher scattering ability in comparison with bare nanostars. Viability assays confirmed weak in vitro toxicity of nanostructures at up to ∼200-μg/mL concentrations. We showed real-time visualization of nanostars in both agarose and cultured cells by analyzing the backscattering signal using a conventional laser confocal microscope. The signal intensity detected from the silica-coated NPs was almost 1.5 times higher in comparison with bare nanostars. To the best of our knowledge, this is the first time that conventional laser confocal microscopy was applied in combined scattering and transmitted light modes to detect the backscattered signal of gold nanostars, which is useful for direct monitoring of the uptake, translocation, and accumulation of NPs in living cells.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.20.7.076017</identifier><identifier>PMID: 26230637</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Backscattering ; Cell Survival - drug effects ; Confocal ; Cytological Techniques - methods ; Gold ; Gold - chemistry ; Gold - toxicity ; HeLa Cells ; Humans ; Imaging ; Lasers ; Microscopy, Confocal ; Nanostructure ; Nanostructures - chemistry ; Nanostructures - toxicity ; Particle Size ; Scattering ; Silicon Dioxide - chemistry ; Silicon Dioxide - toxicity ; Tomography, Optical Coherence - methods ; Transmittance</subject><ispartof>Journal of biomedical optics, 2015-07, Vol.20 (7), p.076017-076017</ispartof><rights>2015 Society of Photo-Optical Instrumentation Engineers (SPIE)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-7f113b7740d445847ac542ec307bdfcbbbc8c2695dacfb56ac81683cf0b291193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26230637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bibikova, Olga</creatorcontrib><creatorcontrib>Popov, Alexey</creatorcontrib><creatorcontrib>Bykov, Alexander</creatorcontrib><creatorcontrib>Prilepskii, Artur</creatorcontrib><creatorcontrib>Kinnunen, Matti</creatorcontrib><creatorcontrib>Kordas, Krisztian</creatorcontrib><creatorcontrib>Bogatyrev, Vladimir</creatorcontrib><creatorcontrib>Khlebtsov, Nikolai</creatorcontrib><creatorcontrib>Vainio, Seppo</creatorcontrib><creatorcontrib>Tuchin, Valery</creatorcontrib><title>Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging</title><title>Journal of biomedical optics</title><addtitle>J. Biomed. Opt</addtitle><description>We synthesized and characterized gold nanostars and their silica-coated derivatives with 7- to 50-nm shell thicknesses as contrast agents for optical imaging. The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflectance/transmittance analyses. The contrasting properties of the nanostructures were studied in optical coherence tomography glass capillary imaging. The silica-coated nanostars with the thickest shell have higher scattering ability in comparison with bare nanostars. Viability assays confirmed weak in vitro toxicity of nanostructures at up to ∼200-μg/mL concentrations. We showed real-time visualization of nanostars in both agarose and cultured cells by analyzing the backscattering signal using a conventional laser confocal microscope. The signal intensity detected from the silica-coated NPs was almost 1.5 times higher in comparison with bare nanostars. To the best of our knowledge, this is the first time that conventional laser confocal microscopy was applied in combined scattering and transmitted light modes to detect the backscattered signal of gold nanostars, which is useful for direct monitoring of the uptake, translocation, and accumulation of NPs in living cells.</description><subject>Backscattering</subject><subject>Cell Survival - drug effects</subject><subject>Confocal</subject><subject>Cytological Techniques - methods</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Gold - toxicity</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Imaging</subject><subject>Lasers</subject><subject>Microscopy, Confocal</subject><subject>Nanostructure</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - toxicity</subject><subject>Particle Size</subject><subject>Scattering</subject><subject>Silicon Dioxide - chemistry</subject><subject>Silicon Dioxide - toxicity</subject><subject>Tomography, Optical Coherence - methods</subject><subject>Transmittance</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtP3DAUhS1U1Blof0A3lZfdJPjaie0shylPIQ0LWFaW7dgoKBMHO1m0v76Goa1UQGLlx_3O0b33IPQFSAkA4gjKy-NNSUkpSiI4AbGHllBzUlAq4UO-E8kKxrlcoIOU7gkhkjf8I1pQThnhTCzRj804dVb3eIxhdHHqXMLB47HXaRuGIroUBj1M2OjosB5anLo-84UNenItzrWQJh0TnlN--hCxdX2Pu62-64a7T2jf6z65z8_nIbo9PblZnxdXm7OL9eqqsFXDpkJ4AGaEqEhbVbWshLZ1RZ1lRJjWW2OMlZbypm619abm2krgkllPDG0AGnaIvu188xQPs0uT2nbpsRE9uDAnBaKqsoABfwdKQDYgGM0o7FAbQ0rReTXGPFj8qYCoxwAUqByAokQJtQsga74-289m69q_ij8bz8DNDkhj59R9mOOQN_PP51c3_m_69LfK2djeXX8_fVEeW59ty9ds3270N9Ilqyk</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Bibikova, Olga</creator><creator>Popov, Alexey</creator><creator>Bykov, Alexander</creator><creator>Prilepskii, Artur</creator><creator>Kinnunen, Matti</creator><creator>Kordas, Krisztian</creator><creator>Bogatyrev, Vladimir</creator><creator>Khlebtsov, Nikolai</creator><creator>Vainio, Seppo</creator><creator>Tuchin, Valery</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20150701</creationdate><title>Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging</title><author>Bibikova, Olga ; Popov, Alexey ; Bykov, Alexander ; Prilepskii, Artur ; Kinnunen, Matti ; Kordas, Krisztian ; Bogatyrev, Vladimir ; Khlebtsov, Nikolai ; Vainio, Seppo ; Tuchin, Valery</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-7f113b7740d445847ac542ec307bdfcbbbc8c2695dacfb56ac81683cf0b291193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Backscattering</topic><topic>Cell Survival - drug effects</topic><topic>Confocal</topic><topic>Cytological Techniques - methods</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Gold - toxicity</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Imaging</topic><topic>Lasers</topic><topic>Microscopy, Confocal</topic><topic>Nanostructure</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - toxicity</topic><topic>Particle Size</topic><topic>Scattering</topic><topic>Silicon Dioxide - chemistry</topic><topic>Silicon Dioxide - toxicity</topic><topic>Tomography, Optical Coherence - methods</topic><topic>Transmittance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bibikova, Olga</creatorcontrib><creatorcontrib>Popov, Alexey</creatorcontrib><creatorcontrib>Bykov, Alexander</creatorcontrib><creatorcontrib>Prilepskii, Artur</creatorcontrib><creatorcontrib>Kinnunen, Matti</creatorcontrib><creatorcontrib>Kordas, Krisztian</creatorcontrib><creatorcontrib>Bogatyrev, Vladimir</creatorcontrib><creatorcontrib>Khlebtsov, Nikolai</creatorcontrib><creatorcontrib>Vainio, Seppo</creatorcontrib><creatorcontrib>Tuchin, Valery</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bibikova, Olga</au><au>Popov, Alexey</au><au>Bykov, Alexander</au><au>Prilepskii, Artur</au><au>Kinnunen, Matti</au><au>Kordas, Krisztian</au><au>Bogatyrev, Vladimir</au><au>Khlebtsov, Nikolai</au><au>Vainio, Seppo</au><au>Tuchin, Valery</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging</atitle><jtitle>Journal of biomedical optics</jtitle><addtitle>J. Biomed. Opt</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>20</volume><issue>7</issue><spage>076017</spage><epage>076017</epage><pages>076017-076017</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>We synthesized and characterized gold nanostars and their silica-coated derivatives with 7- to 50-nm shell thicknesses as contrast agents for optical imaging. The scattering and absorption coefficients of the nanoparticles (NPs) were estimated by means of collimated transmittance and diffuse reflectance/transmittance analyses. The contrasting properties of the nanostructures were studied in optical coherence tomography glass capillary imaging. The silica-coated nanostars with the thickest shell have higher scattering ability in comparison with bare nanostars. Viability assays confirmed weak in vitro toxicity of nanostructures at up to ∼200-μg/mL concentrations. We showed real-time visualization of nanostars in both agarose and cultured cells by analyzing the backscattering signal using a conventional laser confocal microscope. The signal intensity detected from the silica-coated NPs was almost 1.5 times higher in comparison with bare nanostars. To the best of our knowledge, this is the first time that conventional laser confocal microscopy was applied in combined scattering and transmitted light modes to detect the backscattered signal of gold nanostars, which is useful for direct monitoring of the uptake, translocation, and accumulation of NPs in living cells.</abstract><cop>United States</cop><pub>Society of Photo-Optical Instrumentation Engineers</pub><pmid>26230637</pmid><doi>10.1117/1.JBO.20.7.076017</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Backscattering Cell Survival - drug effects Confocal Cytological Techniques - methods Gold Gold - chemistry Gold - toxicity HeLa Cells Humans Imaging Lasers Microscopy, Confocal Nanostructure Nanostructures - chemistry Nanostructures - toxicity Particle Size Scattering Silicon Dioxide - chemistry Silicon Dioxide - toxicity Tomography, Optical Coherence - methods Transmittance |
| title | Optical properties of plasmon-resonant bare and silica-coated nanostars used for cell imaging |
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