A Rapid and Quantitative Detection of Cellular Protein Kinase Activity Based on MALDI-TOF-MS

A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly comp...

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Veröffentlicht in:	Chemistry letters 2014-05, Vol.43 (5), p.658-660
Hauptverfasser:	Otsubo, Yuki, Ikeda, Hiromu, Kamimoto, Junpei, Niidome, Takuro, Mori, Takeshi, Katayama, Yoshiki
Format:	Artikel
Sprache:	eng
Schlagworte:	Cellular Drugs Isotopes Kinases Labels Marking Peptides Proteins
Online-Zugang:	Volltext
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