Correction of peripheral blood mononuclear cell cytosolic protein for hemoglobin contamination

Pharmacodynamic (PD) analysis requires accurate and precise quantification of enzyme activity targeted by anticancer agents in surrogate cells like peripheral blood mononuclear cells (PBMCs). Enzyme activity is normally reported per mass unit of protein input. However, high and fluctuating hemoglobi...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2013-03, Vol.405 (7), p.2391-2395
Hauptverfasser:	Pluim, Dick, Jacobs, Bart A. W., Krähenbühl, Martin D., Ruijter, Anna E. M., Beijnen, Jos H., Schellens, Jan H. M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Anticancer properties Antimitotic agents Antineoplastic agents Biochemistry Blood Blood cells Cancer Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cold Contamination Cytosol - chemistry Cytosol - enzymology Dehydrogenases Dihydrouracil Dehydrogenase (NADP) - analysis Dosage and administration Enzymatic activity Enzyme activity Enzymes Female Fluctuation Food Science Health aspects Hemoglobin Hemoglobins - analysis Humans Identification and classification Laboratory Medicine Leukocytes, Mononuclear - chemistry Leukocytes, Mononuclear - enzymology Male Mathematical analysis Methods Monitoring/Environmental Analysis Pharmacodynamics Pharmacokinetics Physiological aspects Proteins Proteins - analysis Spectrophotometry Spectrophotometry - methods Technical Note
Online-Zugang:	Volltext
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