Synergistic Effect of Hydrogen Peroxide and Elastase on Elastic Fiber Injury In Vitro
This laboratory has previously shown that hyperoxia enhances airspace enlargement in a hamster model of elastase-induced emphysema. To further understand the mechanism responsible for this finding, the effect of oxidants on elastase activity was studied in vitro, using a radiolabeled elastic fiber m...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 2006-01, Vol.231 (1), p.107-111 |
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| creator | Cantor, Jerome O. Shteyngart, Bronislava Cerreta, Joseph M. Ma, Shuren Turino, Gerard M. |
| description | This laboratory has previously shown that hyperoxia enhances airspace enlargement in a hamster model of elastase-induced emphysema. To further understand the mechanism responsible for this finding, the effect of oxidants on elastase activity was studied in vitro, using a radiolabeled elastic fiber matrix derived from rat pleural mesothelial cells. Matrix samples were treated with either 0.1%, 1%, 3%, or 10% hydrogen peroxide (H2O2) for 1 hr, then incubated with 1.0 μg/ml porcine pancreatic elastase for 2 hrs. Radioactivity released from the matrix was used as a measure of elastolysis. Results indicate that sequential exposure to H2O2 and elastase markedly enhanced elastolysis compared to enzyme treatment alone. A 22% increase in elastolysis was seen with 0.1% H2O2 (325 vs. 396 cpm; P < 0.05), whereas samples pretreated with 1%, 3%, and 10% H2O2 showed increases of 53% (274 vs. 420 cpm; P < 0.05), 71% (381 vs. 653 cpm; P < 0.01), and 38% (322 vs. 443 cpm; P < 0.01), respectively. Exposure to various concentrations of H2O2 alone (0.1% to 10%) produced only minimal elastolysis ( |
| doi_str_mv | 10.1177/153537020623100113 |
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To further understand the mechanism responsible for this finding, the effect of oxidants on elastase activity was studied in vitro, using a radiolabeled elastic fiber matrix derived from rat pleural mesothelial cells. Matrix samples were treated with either 0.1%, 1%, 3%, or 10% hydrogen peroxide (H2O2) for 1 hr, then incubated with 1.0 μg/ml porcine pancreatic elastase for 2 hrs. Radioactivity released from the matrix was used as a measure of elastolysis. Results indicate that sequential exposure to H2O2 and elastase markedly enhanced elastolysis compared to enzyme treatment alone. A 22% increase in elastolysis was seen with 0.1% H2O2 (325 vs. 396 cpm; P < 0.05), whereas samples pretreated with 1%, 3%, and 10% H2O2 showed increases of 53% (274 vs. 420 cpm; P < 0.05), 71% (381 vs. 653 cpm; P < 0.01), and 38% (322 vs. 443 cpm; P < 0.01), respectively. Exposure to various concentrations of H2O2 alone (0.1% to 10%) produced only minimal elastolysis (<20 cpm). However, 1% H2O2 was capable of degrading peptide-free desmosine and isodesmosine, suggesting that exposure to this oxidant may reduce the stability of the elastic fiber matrix. With regard to lung diseases such as emphysema, H2O2 and other oxidants derived from inflammatory cells or the environment could possibly act as priming agents for elastase-mediated breakdown of elastic fibers, resulting in amplification of lung injury.</description><identifier>ISSN: 1535-3702</identifier><identifier>ISSN: 1535-3699</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1535-3702</identifier><identifier>DOI: 10.1177/153537020623100113</identifier><identifier>PMID: 16380651</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Cells, Cultured ; Desmosine - metabolism ; Drug Synergism ; Elastic Tissue - drug effects ; Elastic Tissue - injuries ; Elastic Tissue - metabolism ; Extracellular Matrix - drug effects ; Extracellular Matrix - metabolism ; Hydrogen Peroxide - toxicity ; Inflammation Mediators - metabolism ; Isodesmosine - metabolism ; Isotope Labeling ; Oxidants - pharmacology ; Oxidants - toxicity ; Pancreatic Elastase - toxicity ; Pleural Neoplasms - metabolism ; Pleural Neoplasms - pathology ; Pulmonary Emphysema - chemically induced ; Pulmonary Emphysema - metabolism ; Pulmonary Emphysema - pathology ; Rats ; Time Factors</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 2006-01, Vol.231 (1), p.107-111</ispartof><rights>2006 by the Society for Experimental Biology and Medicine</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-36f20e130fc6605b597b624fd46211e3b68fffea51c965aded24b335204fa2193</citedby><cites>FETCH-LOGICAL-c372t-36f20e130fc6605b597b624fd46211e3b68fffea51c965aded24b335204fa2193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16380651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cantor, Jerome O.</creatorcontrib><creatorcontrib>Shteyngart, Bronislava</creatorcontrib><creatorcontrib>Cerreta, Joseph M.</creatorcontrib><creatorcontrib>Ma, Shuren</creatorcontrib><creatorcontrib>Turino, Gerard M.</creatorcontrib><title>Synergistic Effect of Hydrogen Peroxide and Elastase on Elastic Fiber Injury In Vitro</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Exp Biol Med (Maywood)</addtitle><description>This laboratory has previously shown that hyperoxia enhances airspace enlargement in a hamster model of elastase-induced emphysema. To further understand the mechanism responsible for this finding, the effect of oxidants on elastase activity was studied in vitro, using a radiolabeled elastic fiber matrix derived from rat pleural mesothelial cells. Matrix samples were treated with either 0.1%, 1%, 3%, or 10% hydrogen peroxide (H2O2) for 1 hr, then incubated with 1.0 μg/ml porcine pancreatic elastase for 2 hrs. Radioactivity released from the matrix was used as a measure of elastolysis. Results indicate that sequential exposure to H2O2 and elastase markedly enhanced elastolysis compared to enzyme treatment alone. A 22% increase in elastolysis was seen with 0.1% H2O2 (325 vs. 396 cpm; P < 0.05), whereas samples pretreated with 1%, 3%, and 10% H2O2 showed increases of 53% (274 vs. 420 cpm; P < 0.05), 71% (381 vs. 653 cpm; P < 0.01), and 38% (322 vs. 443 cpm; P < 0.01), respectively. Exposure to various concentrations of H2O2 alone (0.1% to 10%) produced only minimal elastolysis (<20 cpm). However, 1% H2O2 was capable of degrading peptide-free desmosine and isodesmosine, suggesting that exposure to this oxidant may reduce the stability of the elastic fiber matrix. With regard to lung diseases such as emphysema, H2O2 and other oxidants derived from inflammatory cells or the environment could possibly act as priming agents for elastase-mediated breakdown of elastic fibers, resulting in amplification of lung injury.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Desmosine - metabolism</subject><subject>Drug Synergism</subject><subject>Elastic Tissue - drug effects</subject><subject>Elastic Tissue - injuries</subject><subject>Elastic Tissue - metabolism</subject><subject>Extracellular Matrix - drug effects</subject><subject>Extracellular Matrix - metabolism</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>Inflammation Mediators - metabolism</subject><subject>Isodesmosine - metabolism</subject><subject>Isotope Labeling</subject><subject>Oxidants - pharmacology</subject><subject>Oxidants - toxicity</subject><subject>Pancreatic Elastase - toxicity</subject><subject>Pleural Neoplasms - metabolism</subject><subject>Pleural Neoplasms - pathology</subject><subject>Pulmonary Emphysema - chemically induced</subject><subject>Pulmonary Emphysema - metabolism</subject><subject>Pulmonary Emphysema - pathology</subject><subject>Rats</subject><subject>Time Factors</subject><issn>1535-3702</issn><issn>1535-3699</issn><issn>1535-3699</issn><issn>1535-3702</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFLw0AQhRdRbK3-AQ-yJ2-1O7vZTXOU0tpCQUHrNWyS2ZKSZutuAubfd0sqHgRP8xi-95h5hNwDewKI4wlIIUXMOFNcAGMA4oIMT8uxUEly-aMDMSA33u8CImOurskAlJgyJWFINu9djW5b-qbM6dwYzBtqDV12hbNbrOkbOvtdFkh1XdB5pX2jPVJb9zp4FmWGjq7qXeu6MOhn2Th7S66MrjzeneeIbBbzj9lyvH59Wc2e1-NcxLwJZxrOEAQzuVJMZjKJM8UjU0SKA6DI1NSEk7SEPFFSF1jwKBNCchYZzSERI_LY5x6c_WrRN-m-9DlWla7Rtj6FOIqU5DyAvAdzZ713aNKDK_fadSmw9FRm-rfMYHo4p7fZHotfy7m9AEx6wOstpjvbujp8-1_kERbGezI</recordid><startdate>200601</startdate><enddate>200601</enddate><creator>Cantor, Jerome O.</creator><creator>Shteyngart, Bronislava</creator><creator>Cerreta, Joseph M.</creator><creator>Ma, Shuren</creator><creator>Turino, Gerard M.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>200601</creationdate><title>Synergistic Effect of Hydrogen Peroxide and Elastase on Elastic Fiber Injury In Vitro</title><author>Cantor, Jerome O. ; Shteyngart, Bronislava ; Cerreta, Joseph M. ; Ma, Shuren ; Turino, Gerard M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-36f20e130fc6605b597b624fd46211e3b68fffea51c965aded24b335204fa2193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Desmosine - metabolism</topic><topic>Drug Synergism</topic><topic>Elastic Tissue - drug effects</topic><topic>Elastic Tissue - injuries</topic><topic>Elastic Tissue - metabolism</topic><topic>Extracellular Matrix - drug effects</topic><topic>Extracellular Matrix - metabolism</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>Inflammation Mediators - metabolism</topic><topic>Isodesmosine - metabolism</topic><topic>Isotope Labeling</topic><topic>Oxidants - pharmacology</topic><topic>Oxidants - toxicity</topic><topic>Pancreatic Elastase - toxicity</topic><topic>Pleural Neoplasms - metabolism</topic><topic>Pleural Neoplasms - pathology</topic><topic>Pulmonary Emphysema - chemically induced</topic><topic>Pulmonary Emphysema - metabolism</topic><topic>Pulmonary Emphysema - pathology</topic><topic>Rats</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cantor, Jerome O.</creatorcontrib><creatorcontrib>Shteyngart, Bronislava</creatorcontrib><creatorcontrib>Cerreta, Joseph M.</creatorcontrib><creatorcontrib>Ma, Shuren</creatorcontrib><creatorcontrib>Turino, Gerard M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cantor, Jerome O.</au><au>Shteyngart, Bronislava</au><au>Cerreta, Joseph M.</au><au>Ma, Shuren</au><au>Turino, Gerard M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergistic Effect of Hydrogen Peroxide and Elastase on Elastic Fiber Injury In Vitro</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Exp Biol Med (Maywood)</addtitle><date>2006-01</date><risdate>2006</risdate><volume>231</volume><issue>1</issue><spage>107</spage><epage>111</epage><pages>107-111</pages><issn>1535-3702</issn><issn>1535-3699</issn><eissn>1535-3699</eissn><eissn>1535-3702</eissn><abstract>This laboratory has previously shown that hyperoxia enhances airspace enlargement in a hamster model of elastase-induced emphysema. To further understand the mechanism responsible for this finding, the effect of oxidants on elastase activity was studied in vitro, using a radiolabeled elastic fiber matrix derived from rat pleural mesothelial cells. Matrix samples were treated with either 0.1%, 1%, 3%, or 10% hydrogen peroxide (H2O2) for 1 hr, then incubated with 1.0 μg/ml porcine pancreatic elastase for 2 hrs. Radioactivity released from the matrix was used as a measure of elastolysis. Results indicate that sequential exposure to H2O2 and elastase markedly enhanced elastolysis compared to enzyme treatment alone. A 22% increase in elastolysis was seen with 0.1% H2O2 (325 vs. 396 cpm; P < 0.05), whereas samples pretreated with 1%, 3%, and 10% H2O2 showed increases of 53% (274 vs. 420 cpm; P < 0.05), 71% (381 vs. 653 cpm; P < 0.01), and 38% (322 vs. 443 cpm; P < 0.01), respectively. Exposure to various concentrations of H2O2 alone (0.1% to 10%) produced only minimal elastolysis (<20 cpm). However, 1% H2O2 was capable of degrading peptide-free desmosine and isodesmosine, suggesting that exposure to this oxidant may reduce the stability of the elastic fiber matrix. With regard to lung diseases such as emphysema, H2O2 and other oxidants derived from inflammatory cells or the environment could possibly act as priming agents for elastase-mediated breakdown of elastic fibers, resulting in amplification of lung injury.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>16380651</pmid><doi>10.1177/153537020623100113</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Cells, Cultured Desmosine - metabolism Drug Synergism Elastic Tissue - drug effects Elastic Tissue - injuries Elastic Tissue - metabolism Extracellular Matrix - drug effects Extracellular Matrix - metabolism Hydrogen Peroxide - toxicity Inflammation Mediators - metabolism Isodesmosine - metabolism Isotope Labeling Oxidants - pharmacology Oxidants - toxicity Pancreatic Elastase - toxicity Pleural Neoplasms - metabolism Pleural Neoplasms - pathology Pulmonary Emphysema - chemically induced Pulmonary Emphysema - metabolism Pulmonary Emphysema - pathology Rats Time Factors |
| title | Synergistic Effect of Hydrogen Peroxide and Elastase on Elastic Fiber Injury In Vitro |
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