Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA

The phylogenetic relationships between Bombycidae ( Bombyx mori and Bombyx mandarina) and Saturniidae ( Antheraea yamamai and Antheraea pernyi) were investigated based on large and small mitochondiral rRNA genes. About 430 bp of four kinds of PCR-amplified fragments were sequenced and aligned. For t...

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Veröffentlicht in:Genetic analysis : biomolecular engineering 1999-12, Vol.15 (6), p.223-228
Hauptverfasser: Hwang, Jae-Sam, Lee, Jin-Sung, Goo, Tae-Won, Young Yun, Eun, Sohn, Hae-Ryong, Rak Kim, Ho, Kwon, O-Yu
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container_end_page 228
container_issue 6
container_start_page 223
container_title Genetic analysis : biomolecular engineering
container_volume 15
creator Hwang, Jae-Sam
Lee, Jin-Sung
Goo, Tae-Won
Young Yun, Eun
Sohn, Hae-Ryong
Rak Kim, Ho
Kwon, O-Yu
description The phylogenetic relationships between Bombycidae ( Bombyx mori and Bombyx mandarina) and Saturniidae ( Antheraea yamamai and Antheraea pernyi) were investigated based on large and small mitochondiral rRNA genes. About 430 bp of four kinds of PCR-amplified fragments were sequenced and aligned. For the 16S rRNA gene, B. mori shared a 98, 87 and 86% sequence homology with B. mandarina, A. yamamai and A. pernyi, and for the 12S rRNA gene, B. mori shared a 99, 89 and 88% sequence homology with B. mandarina, A. yamamai and A. pernyi, respectively. DNA sequence data were also used for a phylogenetic analysis. All of the trees showed monophyly for both Bombycidae and Saturniidae. The monophyly confidence limits of these trees were estimated using bootstrapping tests and measured more than 99% for all trees for both Bombycidae and Saturniidae.
doi_str_mv 10.1016/S1050-3862(99)00008-X
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subjects Animals
Antheraea pernyi
Antheraea yamamai
Base Sequence
Bombyx - classification
Bombyx - genetics
Bombyx mandarina
Bombyx mori
DNA, Mitochondrial - genetics
DNA, Ribosomal - genetics
Molecular Sequence Data
Moths - classification
Moths - genetics
Phylogenetic relationships
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal - genetics
RNA, Ribosomal, 16S - genetics
rRNA 12S
rRNA 16S
rRNA gene
Saturniidae
Sequence Alignment
title Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA
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