Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive
Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosp...
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Veröffentlicht in: | Biotechnology letters 1999-11, Vol.21 (11), p.925-927 |
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creator | KIM, D.-H OH, B.-C CHOI, W.-C LEE, J.-K OH, T.-K |
description | Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl^sub 2^ significantly broadened the active pH range and also increased the pH stability of DS11 phytase.[PUBLICATION ABSTRACT] |
doi_str_mv | 10.1023/A:1005602717835 |
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subjects | Additives Aspergillus ficuum Bacillus amyloliquefaciens Biological and medical sciences Enzymes Feed additives Feed and pet food industries Food industries Fundamental and applied biological sciences. Psychology Phosphates |
title | Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive |
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