Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive

Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosp...

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Veröffentlicht in:Biotechnology letters 1999-11, Vol.21 (11), p.925-927
Hauptverfasser: KIM, D.-H, OH, B.-C, CHOI, W.-C, LEE, J.-K, OH, T.-K
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container_end_page 927
container_issue 11
container_start_page 925
container_title Biotechnology letters
container_volume 21
creator KIM, D.-H
OH, B.-C
CHOI, W.-C
LEE, J.-K
OH, T.-K
description Phytases from Bacillus amyloliquefaciens DS11 and Aspergillus ficuum for feed enzyme were compared on the basis of phosphate inhibition and thermal stabilities. The apparent half-life of the former enzyme at 80 °C was 42 min. The activity of B. amyloliquefaciens phytase was retained up to 5 mM phosphate while 3 mM phosphate inhibited 50% of the original activity in case of A. ficuum phytase. Addition of 5 mM CaCl^sub 2^ significantly broadened the active pH range and also increased the pH stability of DS11 phytase.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/A:1005602717835
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subjects Additives
Aspergillus ficuum
Bacillus amyloliquefaciens
Biological and medical sciences
Enzymes
Feed additives
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
Phosphates
title Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive
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