A stable and highly sensitive 3,3′,5,5′-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays

3,3′,5,5′-Tetramethylbenzidine (TMB) is a widely used chromogen for horseradish peroxidase-based detection systems because it yields reaction products with high absorption coefficients and lacks carcinogenicity. Unfortunately, TMB is labile and poorly soluble in aqueous buffers and such solutions mu...

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Veröffentlicht in:	Journal of immunological methods 2000-01, Vol.233 (1), p.47-56
Hauptverfasser:	Frey, Andreas, Meckelein, Barbara, Externest, Dörthe, Schmidt, M.Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	3,3',5,5'-tetramethylbenzidine Animals Benzidines Biological and medical sciences Chromogenic Compounds Drug Stability Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - statistics & numerical data Evaluation Studies as Topic Female Fundamental and applied biological sciences. Psychology Fundamental immunology Horseradish Peroxidase Immunoassay Immunoglobulin A - analysis Immunoglobulin G - analysis Indicators and Reagents Mice Mice, Inbred BALB C Molecular immunology Ovalbumin - immunology Sensitivity Sensitivity and Specificity Substrate reagent Substrate Specificity Techniques
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