Increased Expression of Myosin Light Chain Kinase mRNA Is Related to Metastasis in Non-Small Cell Lung Cancer
Background: The invasiveness of tumor cells depends in large part on their motility, which in turn depends on cytoskeletal function. A major cytoskeletal component involved in cell motility is myosin II, the classical form of myosin first identified in muscle but also expressed in nonmuscle cells. M...
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| Veröffentlicht in: | Tumor biology 2005-05, Vol.26 (3), p.153-157 |
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| creator | Minamiya, Yoshihiro Nakagawa, Taku Saito, Hajime Matsuzaki, Ikuo Taguchi, Kousei Ito, Manabu Ogawa, Jun-ichi |
| description | Background: The invasiveness of tumor cells depends in large part on their motility, which in turn depends on cytoskeletal function. A major cytoskeletal component involved in cell motility is myosin II, the classical form of myosin first identified in muscle but also expressed in nonmuscle cells. Myosin II is activated by myosin light chain kinase (MLCK), which phosphorylates it on its regulatory light chain. In this context, the contribution made by MLCK to tumor cell motility and invasiveness has been investigated extensively in vitro, but clinical evidence of such a contribution has been lacking. In the present study, therefore, we examined the role of MLCK in the metastasis of non-small cell lung cancer (NSCLC) in a clinical setting. Methods: We measured MLCK mRNA expression in tumor samples from 39 NSCLC patients using real-time semiquantitative reverse transcription polymerase chain reaction carried out in a LightCycler. We then correlated MLCK mRNA expression with known clinicopathological factors. Results: We found that levels of MLCK mRNA expression were higher in patients who showed disease recurrence and distant metastasis than in those who did not. Moreover, the 3-year disease-free survival rate among patients showing lower levels of MLCK mRNA expression [log 10 (MLCK/GAPDH) |
| doi_str_mv | 10.1159/000086487 |
| format | Article |
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A major cytoskeletal component involved in cell motility is myosin II, the classical form of myosin first identified in muscle but also expressed in nonmuscle cells. Myosin II is activated by myosin light chain kinase (MLCK), which phosphorylates it on its regulatory light chain. In this context, the contribution made by MLCK to tumor cell motility and invasiveness has been investigated extensively in vitro, but clinical evidence of such a contribution has been lacking. In the present study, therefore, we examined the role of MLCK in the metastasis of non-small cell lung cancer (NSCLC) in a clinical setting. Methods: We measured MLCK mRNA expression in tumor samples from 39 NSCLC patients using real-time semiquantitative reverse transcription polymerase chain reaction carried out in a LightCycler. We then correlated MLCK mRNA expression with known clinicopathological factors. Results: We found that levels of MLCK mRNA expression were higher in patients who showed disease recurrence and distant metastasis than in those who did not. Moreover, the 3-year disease-free survival rate among patients showing lower levels of MLCK mRNA expression [log 10 (MLCK/GAPDH) <1.4] was significantly greater than among those showing higher MLCK mRNA expression [log 10 (MLCK/GAPDH) ≧1.4] (87.5 vs. 50.0%; log-rank test, p = 0.021). Conclusion: These findings are the first clinical evidence that expression of MLCK is correlated with disease recurrence and distant metastasis in NSCLC.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1159/000086487</identifier><identifier>PMID: 15970650</identifier><language>eng</language><publisher>Basel, Switzerland: Springer Nature B.V</publisher><subject>Adult ; Aged ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - secondary ; Case-Control Studies ; Disease-Free Survival ; Female ; Humans ; Lung cancer ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Male ; Middle Aged ; Myosin-Light-Chain Kinase - biosynthesis ; Myosin-Light-Chain Kinase - physiology ; Neoplasm Metastasis ; Neoplasm Recurrence, Local ; Prognosis ; Research Article ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - biosynthesis</subject><ispartof>Tumor biology, 2005-05, Vol.26 (3), p.153-157</ispartof><rights>2005 S. Karger AG, Basel</rights><rights>Copyright (c) 2005 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-19670a84a2f6940a01544860bfe9c0ff965ac4a09d510bd3d1496aad0f7c68a73</citedby><cites>FETCH-LOGICAL-c361t-19670a84a2f6940a01544860bfe9c0ff965ac4a09d510bd3d1496aad0f7c68a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2427,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15970650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minamiya, Yoshihiro</creatorcontrib><creatorcontrib>Nakagawa, Taku</creatorcontrib><creatorcontrib>Saito, Hajime</creatorcontrib><creatorcontrib>Matsuzaki, Ikuo</creatorcontrib><creatorcontrib>Taguchi, Kousei</creatorcontrib><creatorcontrib>Ito, Manabu</creatorcontrib><creatorcontrib>Ogawa, Jun-ichi</creatorcontrib><title>Increased Expression of Myosin Light Chain Kinase mRNA Is Related to Metastasis in Non-Small Cell Lung Cancer</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><description>Background: The invasiveness of tumor cells depends in large part on their motility, which in turn depends on cytoskeletal function. A major cytoskeletal component involved in cell motility is myosin II, the classical form of myosin first identified in muscle but also expressed in nonmuscle cells. Myosin II is activated by myosin light chain kinase (MLCK), which phosphorylates it on its regulatory light chain. In this context, the contribution made by MLCK to tumor cell motility and invasiveness has been investigated extensively in vitro, but clinical evidence of such a contribution has been lacking. In the present study, therefore, we examined the role of MLCK in the metastasis of non-small cell lung cancer (NSCLC) in a clinical setting. Methods: We measured MLCK mRNA expression in tumor samples from 39 NSCLC patients using real-time semiquantitative reverse transcription polymerase chain reaction carried out in a LightCycler. We then correlated MLCK mRNA expression with known clinicopathological factors. Results: We found that levels of MLCK mRNA expression were higher in patients who showed disease recurrence and distant metastasis than in those who did not. Moreover, the 3-year disease-free survival rate among patients showing lower levels of MLCK mRNA expression [log 10 (MLCK/GAPDH) <1.4] was significantly greater than among those showing higher MLCK mRNA expression [log 10 (MLCK/GAPDH) ≧1.4] (87.5 vs. 50.0%; log-rank test, p = 0.021). Conclusion: These findings are the first clinical evidence that expression of MLCK is correlated with disease recurrence and distant metastasis in NSCLC.</description><subject>Adult</subject><subject>Aged</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - secondary</subject><subject>Case-Control Studies</subject><subject>Disease-Free Survival</subject><subject>Female</subject><subject>Humans</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myosin-Light-Chain Kinase - biosynthesis</subject><subject>Myosin-Light-Chain Kinase - physiology</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Recurrence, Local</subject><subject>Prognosis</subject><subject>Research Article</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0c1LwzAUAPAgitPpwbMgwYPgofrSpkl6HMWP4VSYei5vbbpV12QmLeh_b2RDwYsh5L3D7z1eeIQcMbhgLM0uIRwluJJbZI_xOIkgUbAdcmAQ8VglA7Lv_StAwJnYJYMQJYgU9kg7NqXT6HVFrz5WTnvfWENtTe8_rW8MnTTzRUfzBYb8rjEB0nb6MKJjT6d6iV2o6yy91x36cBtPg3uwJnpqcbmkuQ7PpDdzmqMptTsgOzUuvT7cxCF5ub56zm-jyePNOB9NojIRrItYJiSg4hjXIuOAYW7OlYBZrbMS6joTKZYcIatSBrMqqRjPBGIFtSyFQpkMydm678rZ9177rmgbX4Zh0Gjb-0IoiONU8X8hkzxRqfiGp3_gq-2dCZ8o4jgWUkrGAjpfo9JZ752ui5VrWnSfBYPie1PFz6aCPdk07Getrn7lZjUBHK_BG7q5dj9gXf4FxUeUMg</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Minamiya, Yoshihiro</creator><creator>Nakagawa, Taku</creator><creator>Saito, Hajime</creator><creator>Matsuzaki, Ikuo</creator><creator>Taguchi, Kousei</creator><creator>Ito, Manabu</creator><creator>Ogawa, Jun-ichi</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Increased Expression of Myosin Light Chain Kinase mRNA Is Related to Metastasis in Non-Small Cell Lung Cancer</title><author>Minamiya, Yoshihiro ; Nakagawa, Taku ; Saito, Hajime ; Matsuzaki, Ikuo ; Taguchi, Kousei ; Ito, Manabu ; Ogawa, Jun-ichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-19670a84a2f6940a01544860bfe9c0ff965ac4a09d510bd3d1496aad0f7c68a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - secondary</topic><topic>Case-Control Studies</topic><topic>Disease-Free Survival</topic><topic>Female</topic><topic>Humans</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myosin-Light-Chain Kinase - biosynthesis</topic><topic>Myosin-Light-Chain Kinase - physiology</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Recurrence, Local</topic><topic>Prognosis</topic><topic>Research Article</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minamiya, Yoshihiro</creatorcontrib><creatorcontrib>Nakagawa, Taku</creatorcontrib><creatorcontrib>Saito, Hajime</creatorcontrib><creatorcontrib>Matsuzaki, Ikuo</creatorcontrib><creatorcontrib>Taguchi, Kousei</creatorcontrib><creatorcontrib>Ito, Manabu</creatorcontrib><creatorcontrib>Ogawa, Jun-ichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minamiya, Yoshihiro</au><au>Nakagawa, Taku</au><au>Saito, Hajime</au><au>Matsuzaki, Ikuo</au><au>Taguchi, Kousei</au><au>Ito, Manabu</au><au>Ogawa, Jun-ichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Expression of Myosin Light Chain Kinase mRNA Is Related to Metastasis in Non-Small Cell Lung Cancer</atitle><jtitle>Tumor biology</jtitle><addtitle>Tumor Biol</addtitle><date>2005-05</date><risdate>2005</risdate><volume>26</volume><issue>3</issue><spage>153</spage><epage>157</epage><pages>153-157</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>Background: The invasiveness of tumor cells depends in large part on their motility, which in turn depends on cytoskeletal function. A major cytoskeletal component involved in cell motility is myosin II, the classical form of myosin first identified in muscle but also expressed in nonmuscle cells. Myosin II is activated by myosin light chain kinase (MLCK), which phosphorylates it on its regulatory light chain. In this context, the contribution made by MLCK to tumor cell motility and invasiveness has been investigated extensively in vitro, but clinical evidence of such a contribution has been lacking. In the present study, therefore, we examined the role of MLCK in the metastasis of non-small cell lung cancer (NSCLC) in a clinical setting. Methods: We measured MLCK mRNA expression in tumor samples from 39 NSCLC patients using real-time semiquantitative reverse transcription polymerase chain reaction carried out in a LightCycler. We then correlated MLCK mRNA expression with known clinicopathological factors. Results: We found that levels of MLCK mRNA expression were higher in patients who showed disease recurrence and distant metastasis than in those who did not. Moreover, the 3-year disease-free survival rate among patients showing lower levels of MLCK mRNA expression [log 10 (MLCK/GAPDH) <1.4] was significantly greater than among those showing higher MLCK mRNA expression [log 10 (MLCK/GAPDH) ≧1.4] (87.5 vs. 50.0%; log-rank test, p = 0.021). Conclusion: These findings are the first clinical evidence that expression of MLCK is correlated with disease recurrence and distant metastasis in NSCLC.</abstract><cop>Basel, Switzerland</cop><pub>Springer Nature B.V</pub><pmid>15970650</pmid><doi>10.1159/000086487</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Tumor biology, 2005-05, Vol.26 (3), p.153-157 |
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| subjects | Adult Aged Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - secondary Case-Control Studies Disease-Free Survival Female Humans Lung cancer Lung Neoplasms - genetics Lung Neoplasms - pathology Male Middle Aged Myosin-Light-Chain Kinase - biosynthesis Myosin-Light-Chain Kinase - physiology Neoplasm Metastasis Neoplasm Recurrence, Local Prognosis Research Article Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis |
| title | Increased Expression of Myosin Light Chain Kinase mRNA Is Related to Metastasis in Non-Small Cell Lung Cancer |
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