Effect of culture conditions on growth and esterase production by the moderate thermophile Bacillus circulans MAS2
Growth and esterase production (activity on p-nitrophenyl caprylate) by the newly isolated Bacillus circulans MAS2 bacterial strain were studied. The growth rate at 50 degrees was high (0.9 h-1) on LB medium with glucose added. Esterase production followed growth with the majority of activity being...
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| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 1999-09, Vol.23 (3), p.188-193 |
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| creator | Kademi, A Fakhreddine, L Ait-Abdelkader, N Baratti, J.C |
| description | Growth and esterase production (activity on p-nitrophenyl caprylate) by the newly isolated Bacillus circulans MAS2 bacterial strain were studied. The growth rate at 50 degrees was high (0.9 h-1) on LB medium with glucose added. Esterase production followed growth with the majority of activity being intracellular during exponential growth phase. During stationary phase, the esterase activity was released in the culture medium. The strain was able to grow at 35-55 degrees C with maximum growth rate at 50 degrees C, showing a pattern typical of a moderate thermophile. Growth occurred at pH 6-9 with a maximum at 8, with a similar pattern for the esterase production. Addition of glucose, fructose, sucrose or sodium acetate greatly promoted both growth and esterase production while starch, inulin, tributyrin or glycerol showed no effect. Complex nitrogen sources such as tryptone or yeast extract increased growth and esterase production while mineral sources (ammonium chloride or sulfate), glycine or glutamate showed no effect. An increase of tryptone plus yeast extract and glucose concentrations stimulated growth and esterase production which reached 160 UL-1. |
| doi_str_mv | 10.1038/sj.jim.2900717 |
| format | Article |
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The growth rate at 50 degrees was high (0.9 h-1) on LB medium with glucose added. Esterase production followed growth with the majority of activity being intracellular during exponential growth phase. During stationary phase, the esterase activity was released in the culture medium. The strain was able to grow at 35-55 degrees C with maximum growth rate at 50 degrees C, showing a pattern typical of a moderate thermophile. Growth occurred at pH 6-9 with a maximum at 8, with a similar pattern for the esterase production. Addition of glucose, fructose, sucrose or sodium acetate greatly promoted both growth and esterase production while starch, inulin, tributyrin or glycerol showed no effect. Complex nitrogen sources such as tryptone or yeast extract increased growth and esterase production while mineral sources (ammonium chloride or sulfate), glycine or glutamate showed no effect. An increase of tryptone plus yeast extract and glucose concentrations stimulated growth and esterase production which reached 160 UL-1.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1038/sj.jim.2900717</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Ammonium ; ammonium chloride ; Bacillus circulans ; Biological and medical sciences ; Biological Sciences ; Biotechnology ; Carbon ; Chemical products ; Effects ; Enzyme engineering ; Enzymes ; esterase ; Fermentation ; food microbiology ; food processing ; fructose ; Fundamental and applied biological sciences. Psychology ; Glucose ; Glycerol ; Laboratories ; Methods. Procedures. 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The growth rate at 50 degrees was high (0.9 h-1) on LB medium with glucose added. Esterase production followed growth with the majority of activity being intracellular during exponential growth phase. During stationary phase, the esterase activity was released in the culture medium. The strain was able to grow at 35-55 degrees C with maximum growth rate at 50 degrees C, showing a pattern typical of a moderate thermophile. Growth occurred at pH 6-9 with a maximum at 8, with a similar pattern for the esterase production. Addition of glucose, fructose, sucrose or sodium acetate greatly promoted both growth and esterase production while starch, inulin, tributyrin or glycerol showed no effect. Complex nitrogen sources such as tryptone or yeast extract increased growth and esterase production while mineral sources (ammonium chloride or sulfate), glycine or glutamate showed no effect. An increase of tryptone plus yeast extract and glucose concentrations stimulated growth and esterase production which reached 160 UL-1.</description><subject>Ammonium</subject><subject>ammonium chloride</subject><subject>Bacillus circulans</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences</subject><subject>Biotechnology</subject><subject>Carbon</subject><subject>Chemical products</subject><subject>Effects</subject><subject>Enzyme engineering</subject><subject>Enzymes</subject><subject>esterase</subject><subject>Fermentation</subject><subject>food microbiology</subject><subject>food processing</subject><subject>fructose</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Glycerol</subject><subject>Laboratories</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Glucose</topic><topic>Glycerol</topic><topic>Laboratories</topic><topic>Methods. Procedures. 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The growth rate at 50 degrees was high (0.9 h-1) on LB medium with glucose added. Esterase production followed growth with the majority of activity being intracellular during exponential growth phase. During stationary phase, the esterase activity was released in the culture medium. The strain was able to grow at 35-55 degrees C with maximum growth rate at 50 degrees C, showing a pattern typical of a moderate thermophile. Growth occurred at pH 6-9 with a maximum at 8, with a similar pattern for the esterase production. Addition of glucose, fructose, sucrose or sodium acetate greatly promoted both growth and esterase production while starch, inulin, tributyrin or glycerol showed no effect. Complex nitrogen sources such as tryptone or yeast extract increased growth and esterase production while mineral sources (ammonium chloride or sulfate), glycine or glutamate showed no effect. An increase of tryptone plus yeast extract and glucose concentrations stimulated growth and esterase production which reached 160 UL-1.</abstract><cop>Heidelberg</cop><pub>Springer</pub><doi>10.1038/sj.jim.2900717</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of industrial microbiology & biotechnology, 1999-09, Vol.23 (3), p.188-193 |
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| source | Oxford Journals Open Access Collection; SpringerLink Journals - AutoHoldings |
| subjects | Ammonium ammonium chloride Bacillus circulans Biological and medical sciences Biological Sciences Biotechnology Carbon Chemical products Effects Enzyme engineering Enzymes esterase Fermentation food microbiology food processing fructose Fundamental and applied biological sciences. Psychology Glucose Glycerol Laboratories Methods. Procedures. Technologies Nitrogen Nitrogen sources Production of selected enzymes sodium acetate Studies sucrose sulfate Yeast Yeasts |
| title | Effect of culture conditions on growth and esterase production by the moderate thermophile Bacillus circulans MAS2 |
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