Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes
The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sa...
Gespeichert in:
| Veröffentlicht in: | Mutagenesis 1999-11, Vol.14 (6), p.621-632 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 632 |
|---|---|
| container_issue | 6 |
| container_start_page | 621 |
| container_title | Mutagenesis |
| container_volume | 14 |
| creator | de Stoppelaar, Joyce M. van de Kuil, Ton Bedaf, Mirjam Verharen, Henny W. Slob, Wout Mohn, Georges R. Hoebee, Barbara van Benthem, Jan |
| description | The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sacrifice (0, 50, 150, 450 and 800 mg/kg body wt CARB in corn oil), corresponding to exposure during late pachytene, revealed a clear induction of diploid sperm. Induction of aneuploid sperm was not observed. Although the absolute frequencies of diploidy were low, ranging from 0.03% in the control group to 0.22% in the highest dose group, the observed dose–response relationship was highly significant. In sperm of rats killed 50 days after treatment with CARB (corresponding to exposure of spermatogonial stem cells) the effect was no longer apparent. In a second experiment, in addition to more dose groups in the low dose range, the peripheral blood micronucleus assay was incorporated. Results of triple colour FISH on epididymal sperm of rats treated with CARB (0–800 mg/kg body wt) again showed induction of diploid, but not of aneuploid sperm. Induction was less prominent than in the first experiment, but the dose–response relationship for diploidy was again significant. In blood samples drawn from the tail vein 48 h after treatment with CARB induction of micronuclei in peripheral blood erythrocytes was not observed, whereas the micronucleus frequency was significantly increased after a single i.p. dose of mitomycin C (3 mg/kg body wt). In conclusion, the present results show that CARB induces diploidy in sperm, without an accompanying induction of micronuclei in erythrocytes. This finding suggests that in rats the peripheral blood micronucleus assay is a less sensitive indicator for the genotoxic potential of CARB than the epididymal sperm aneuploidy/diploidy assay. |
| doi_str_mv | 10.1093/mutage/14.6.621 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17422068</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>950802631</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-9cdc18d6dc56bb6fe4fee852a25d37e0f73525969b83261dfacb51389f31397f3</originalsourceid><addsrcrecordid>eNpdkV2PEyEUhidG49bVa-8MMca7afkYYObSVNc22cQYP2K8IQwcLOvM0AUmWn-TP1JqGzVeQeA574HzVNVjgpcEd2w1zll_gRVplmIpKLlTLUgjmpq1uLlbLTAVsm4ZlxfVg5RuMCaSCny_uiCYC4lZu6h-bicTQSewyEW4nWEyHhIKDlm_H4K3KO0hjshCBpML1R_QOA_ZmzCEOaKr7bsN0i5DRLnk5BGmfKyOOif0zecdMjr2MFn9w4-_D8Kc0ehNDNNsBpgT8pOdTfZhKjtUmvn9DqIeUD-EYBHEQ97FYA4Z0sPqntNDgkfn9bL6cPXq_XpTX795vV2_uK5Nw7tcd8Ya0lphDRd9Lxw0DqDlVFNumQTsJOOUd6LrW0YFsU6bnhPWdo4R1knHLqvnp9x9DGUkKavRJwPDoCcIc1JENpRi0Rbw6X_gTRnKVN6mKJEtly07QqsTVP6cUgSn9tGPOh4UwepoUZ0sKtIooYrFUvHkHDv3I9h_-JO2Ajw7AzoZPbioi7b0l6MEy04WrD5hPmX4_udax69KSCa52nz6rAR-S9Yfu5eKsl-lxrpX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217857838</pqid></control><display><type>article</type><title>Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>de Stoppelaar, Joyce M. ; van de Kuil, Ton ; Bedaf, Mirjam ; Verharen, Henny W. ; Slob, Wout ; Mohn, Georges R. ; Hoebee, Barbara ; van Benthem, Jan</creator><creatorcontrib>de Stoppelaar, Joyce M. ; van de Kuil, Ton ; Bedaf, Mirjam ; Verharen, Henny W. ; Slob, Wout ; Mohn, Georges R. ; Hoebee, Barbara ; van Benthem, Jan</creatorcontrib><description>The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sacrifice (0, 50, 150, 450 and 800 mg/kg body wt CARB in corn oil), corresponding to exposure during late pachytene, revealed a clear induction of diploid sperm. Induction of aneuploid sperm was not observed. Although the absolute frequencies of diploidy were low, ranging from 0.03% in the control group to 0.22% in the highest dose group, the observed dose–response relationship was highly significant. In sperm of rats killed 50 days after treatment with CARB (corresponding to exposure of spermatogonial stem cells) the effect was no longer apparent. In a second experiment, in addition to more dose groups in the low dose range, the peripheral blood micronucleus assay was incorporated. Results of triple colour FISH on epididymal sperm of rats treated with CARB (0–800 mg/kg body wt) again showed induction of diploid, but not of aneuploid sperm. Induction was less prominent than in the first experiment, but the dose–response relationship for diploidy was again significant. In blood samples drawn from the tail vein 48 h after treatment with CARB induction of micronuclei in peripheral blood erythrocytes was not observed, whereas the micronucleus frequency was significantly increased after a single i.p. dose of mitomycin C (3 mg/kg body wt). In conclusion, the present results show that CARB induces diploidy in sperm, without an accompanying induction of micronuclei in erythrocytes. This finding suggests that in rats the peripheral blood micronucleus assay is a less sensitive indicator for the genotoxic potential of CARB than the epididymal sperm aneuploidy/diploidy assay.</description><identifier>ISSN: 0267-8357</identifier><identifier>EISSN: 1464-3804</identifier><identifier>DOI: 10.1093/mutage/14.6.621</identifier><identifier>PMID: 10567038</identifier><identifier>CODEN: MUTAEX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Administration, Oral ; Aneuploidy ; Animals ; Benzimidazoles - toxicity ; Biological and medical sciences ; Carbamates ; carbendazim ; Diploidy ; Dose-Response Relationship, Drug ; Erythrocytes - drug effects ; In Situ Hybridization, Fluorescence ; Male ; Medical sciences ; Meiosis ; Micronucleus Tests ; Mutagens - toxicity ; Pesticides, fertilizers and other agrochemicals toxicology ; Rats ; Rats, Wistar ; Spermatogonia - drug effects ; Spermatozoa - drug effects ; Toxicology</subject><ispartof>Mutagenesis, 1999-11, Vol.14 (6), p.621-632</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Nov 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-9cdc18d6dc56bb6fe4fee852a25d37e0f73525969b83261dfacb51389f31397f3</citedby><cites>FETCH-LOGICAL-c459t-9cdc18d6dc56bb6fe4fee852a25d37e0f73525969b83261dfacb51389f31397f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1210797$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10567038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Stoppelaar, Joyce M.</creatorcontrib><creatorcontrib>van de Kuil, Ton</creatorcontrib><creatorcontrib>Bedaf, Mirjam</creatorcontrib><creatorcontrib>Verharen, Henny W.</creatorcontrib><creatorcontrib>Slob, Wout</creatorcontrib><creatorcontrib>Mohn, Georges R.</creatorcontrib><creatorcontrib>Hoebee, Barbara</creatorcontrib><creatorcontrib>van Benthem, Jan</creatorcontrib><title>Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes</title><title>Mutagenesis</title><addtitle>Mutagenesis</addtitle><description>The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sacrifice (0, 50, 150, 450 and 800 mg/kg body wt CARB in corn oil), corresponding to exposure during late pachytene, revealed a clear induction of diploid sperm. Induction of aneuploid sperm was not observed. Although the absolute frequencies of diploidy were low, ranging from 0.03% in the control group to 0.22% in the highest dose group, the observed dose–response relationship was highly significant. In sperm of rats killed 50 days after treatment with CARB (corresponding to exposure of spermatogonial stem cells) the effect was no longer apparent. In a second experiment, in addition to more dose groups in the low dose range, the peripheral blood micronucleus assay was incorporated. Results of triple colour FISH on epididymal sperm of rats treated with CARB (0–800 mg/kg body wt) again showed induction of diploid, but not of aneuploid sperm. Induction was less prominent than in the first experiment, but the dose–response relationship for diploidy was again significant. In blood samples drawn from the tail vein 48 h after treatment with CARB induction of micronuclei in peripheral blood erythrocytes was not observed, whereas the micronucleus frequency was significantly increased after a single i.p. dose of mitomycin C (3 mg/kg body wt). In conclusion, the present results show that CARB induces diploidy in sperm, without an accompanying induction of micronuclei in erythrocytes. This finding suggests that in rats the peripheral blood micronucleus assay is a less sensitive indicator for the genotoxic potential of CARB than the epididymal sperm aneuploidy/diploidy assay.</description><subject>Administration, Oral</subject><subject>Aneuploidy</subject><subject>Animals</subject><subject>Benzimidazoles - toxicity</subject><subject>Biological and medical sciences</subject><subject>Carbamates</subject><subject>carbendazim</subject><subject>Diploidy</subject><subject>Dose-Response Relationship, Drug</subject><subject>Erythrocytes - drug effects</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Meiosis</subject><subject>Micronucleus Tests</subject><subject>Mutagens - toxicity</subject><subject>Pesticides, fertilizers and other agrochemicals toxicology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spermatogonia - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Toxicology</subject><issn>0267-8357</issn><issn>1464-3804</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV2PEyEUhidG49bVa-8MMca7afkYYObSVNc22cQYP2K8IQwcLOvM0AUmWn-TP1JqGzVeQeA574HzVNVjgpcEd2w1zll_gRVplmIpKLlTLUgjmpq1uLlbLTAVsm4ZlxfVg5RuMCaSCny_uiCYC4lZu6h-bicTQSewyEW4nWEyHhIKDlm_H4K3KO0hjshCBpML1R_QOA_ZmzCEOaKr7bsN0i5DRLnk5BGmfKyOOif0zecdMjr2MFn9w4-_D8Kc0ehNDNNsBpgT8pOdTfZhKjtUmvn9DqIeUD-EYBHEQ97FYA4Z0sPqntNDgkfn9bL6cPXq_XpTX795vV2_uK5Nw7tcd8Ya0lphDRd9Lxw0DqDlVFNumQTsJOOUd6LrW0YFsU6bnhPWdo4R1knHLqvnp9x9DGUkKavRJwPDoCcIc1JENpRi0Rbw6X_gTRnKVN6mKJEtly07QqsTVP6cUgSn9tGPOh4UwepoUZ0sKtIooYrFUvHkHDv3I9h_-JO2Ajw7AzoZPbioi7b0l6MEy04WrD5hPmX4_udax69KSCa52nz6rAR-S9Yfu5eKsl-lxrpX</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>de Stoppelaar, Joyce M.</creator><creator>van de Kuil, Ton</creator><creator>Bedaf, Mirjam</creator><creator>Verharen, Henny W.</creator><creator>Slob, Wout</creator><creator>Mohn, Georges R.</creator><creator>Hoebee, Barbara</creator><creator>van Benthem, Jan</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19991101</creationdate><title>Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes</title><author>de Stoppelaar, Joyce M. ; van de Kuil, Ton ; Bedaf, Mirjam ; Verharen, Henny W. ; Slob, Wout ; Mohn, Georges R. ; Hoebee, Barbara ; van Benthem, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-9cdc18d6dc56bb6fe4fee852a25d37e0f73525969b83261dfacb51389f31397f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Administration, Oral</topic><topic>Aneuploidy</topic><topic>Animals</topic><topic>Benzimidazoles - toxicity</topic><topic>Biological and medical sciences</topic><topic>Carbamates</topic><topic>carbendazim</topic><topic>Diploidy</topic><topic>Dose-Response Relationship, Drug</topic><topic>Erythrocytes - drug effects</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Meiosis</topic><topic>Micronucleus Tests</topic><topic>Mutagens - toxicity</topic><topic>Pesticides, fertilizers and other agrochemicals toxicology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Spermatogonia - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Stoppelaar, Joyce M.</creatorcontrib><creatorcontrib>van de Kuil, Ton</creatorcontrib><creatorcontrib>Bedaf, Mirjam</creatorcontrib><creatorcontrib>Verharen, Henny W.</creatorcontrib><creatorcontrib>Slob, Wout</creatorcontrib><creatorcontrib>Mohn, Georges R.</creatorcontrib><creatorcontrib>Hoebee, Barbara</creatorcontrib><creatorcontrib>van Benthem, Jan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Mutagenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Stoppelaar, Joyce M.</au><au>van de Kuil, Ton</au><au>Bedaf, Mirjam</au><au>Verharen, Henny W.</au><au>Slob, Wout</au><au>Mohn, Georges R.</au><au>Hoebee, Barbara</au><au>van Benthem, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes</atitle><jtitle>Mutagenesis</jtitle><addtitle>Mutagenesis</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>14</volume><issue>6</issue><spage>621</spage><epage>632</epage><pages>621-632</pages><issn>0267-8357</issn><eissn>1464-3804</eissn><coden>MUTAEX</coden><abstract>The purpose of the present study was to determine the effect of a single oral dose of carbendazim (CARB) on the frequencies of numerical chromosome aberrations in sperm and on micronuclei in peripheral blood erythrocytes of rats. Dual colour FISH on epididymal sperm of rats treated 31 days before sacrifice (0, 50, 150, 450 and 800 mg/kg body wt CARB in corn oil), corresponding to exposure during late pachytene, revealed a clear induction of diploid sperm. Induction of aneuploid sperm was not observed. Although the absolute frequencies of diploidy were low, ranging from 0.03% in the control group to 0.22% in the highest dose group, the observed dose–response relationship was highly significant. In sperm of rats killed 50 days after treatment with CARB (corresponding to exposure of spermatogonial stem cells) the effect was no longer apparent. In a second experiment, in addition to more dose groups in the low dose range, the peripheral blood micronucleus assay was incorporated. Results of triple colour FISH on epididymal sperm of rats treated with CARB (0–800 mg/kg body wt) again showed induction of diploid, but not of aneuploid sperm. Induction was less prominent than in the first experiment, but the dose–response relationship for diploidy was again significant. In blood samples drawn from the tail vein 48 h after treatment with CARB induction of micronuclei in peripheral blood erythrocytes was not observed, whereas the micronucleus frequency was significantly increased after a single i.p. dose of mitomycin C (3 mg/kg body wt). In conclusion, the present results show that CARB induces diploidy in sperm, without an accompanying induction of micronuclei in erythrocytes. This finding suggests that in rats the peripheral blood micronucleus assay is a less sensitive indicator for the genotoxic potential of CARB than the epididymal sperm aneuploidy/diploidy assay.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10567038</pmid><doi>10.1093/mutage/14.6.621</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0267-8357 |
| ispartof | Mutagenesis, 1999-11, Vol.14 (6), p.621-632 |
| issn | 0267-8357 1464-3804 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17422068 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Administration, Oral Aneuploidy Animals Benzimidazoles - toxicity Biological and medical sciences Carbamates carbendazim Diploidy Dose-Response Relationship, Drug Erythrocytes - drug effects In Situ Hybridization, Fluorescence Male Medical sciences Meiosis Micronucleus Tests Mutagens - toxicity Pesticides, fertilizers and other agrochemicals toxicology Rats Rats, Wistar Spermatogonia - drug effects Spermatozoa - drug effects Toxicology |
| title | Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A56%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20frequencies%20of%20diploid%20sperm%20detected%20by%20multicolour%20FISH%20after%20treatment%20of%20rats%20with%20carbendazim%20without%20micronucleus%20induction%20in%20peripheral%20blood%20erythrocytes&rft.jtitle=Mutagenesis&rft.au=de%20Stoppelaar,%20Joyce%20M.&rft.date=1999-11-01&rft.volume=14&rft.issue=6&rft.spage=621&rft.epage=632&rft.pages=621-632&rft.issn=0267-8357&rft.eissn=1464-3804&rft.coden=MUTAEX&rft_id=info:doi/10.1093/mutage/14.6.621&rft_dat=%3Cproquest_cross%3E950802631%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217857838&rft_id=info:pmid/10567038&rfr_iscdi=true |