Cloning of the nitrile hydratase gene from Nocardia sp. in Escherichia coli and Pichia pastoris and its functional expression using site-directed mutagenesis

To obtain a recombinant Rhodococcus or Nocardia with not only higher enzymatic activity but also better operational stability and product-tolerance ability for bioconversion of acrylamide from acrylonitrile, an active and stable expression system of nitrile hydratase (NHase) was tried to construct a...

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Veröffentlicht in:	Enzyme and microbial technology 2004-12, Vol.35 (6), p.557-562
Hauptverfasser:	Shi, Yue, Yu, Huimin, Sun, Xudong, Tian, Zhuoling, Shen, Zhongyao
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylamide Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Nitrile hydratase (NHase) Nocardia Pichia pastoris Recombinant E. coli Rhodococcus Site-directed mutagenesis
Online-Zugang:	Volltext
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