Synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus
In vitro synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus was investigated using α-ketoglutarate, L-ascorbic acid, FeSO 4, S-adenosyl- L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with Proteus vulg...
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| Veröffentlicht in: | FEMS microbiology letters 2000-01, Vol.182 (2), p.313-317 |
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| container_title | FEMS microbiology letters |
| container_volume | 182 |
| creator | Kim, Jeong Keun Kang, Heui-il Chae, Jeong Seok Park, Young-Hoon Choi, Yong-Jin |
| description | In vitro synthesis of cefminox by cell-free extracts of
Streptomyces clavuligerus was investigated using α-ketoglutarate,
L-ascorbic acid, FeSO
4,
S-adenosyl-
L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with
Proteus vulgaris GN 76/C-1 and by high performance liquid chromatography. Although the conversion rate of 7α-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox. |
| doi_str_mv | 10.1016/S0378-1097(99)00607-2 |
| format | Article |
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Streptomyces clavuligerus was investigated using α-ketoglutarate,
L-ascorbic acid, FeSO
4,
S-adenosyl-
L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with
Proteus vulgaris GN 76/C-1 and by high performance liquid chromatography. Although the conversion rate of 7α-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(99)00607-2</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>7-Methoxylation ; a-Ketoglutaric acid ; Ascorbic acid ; Cefminox ; Cell-free extract ; Cephamycin ; Proteus vulgaris GN 76/C-1 ; Streptomyces clavuligerus</subject><ispartof>FEMS microbiology letters, 2000-01, Vol.182 (2), p.313-317</ispartof><rights>2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Kim, Jeong Keun</creatorcontrib><creatorcontrib>Kang, Heui-il</creatorcontrib><creatorcontrib>Chae, Jeong Seok</creatorcontrib><creatorcontrib>Park, Young-Hoon</creatorcontrib><creatorcontrib>Choi, Yong-Jin</creatorcontrib><title>Synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus</title><title>FEMS microbiology letters</title><description>In vitro synthesis of cefminox by cell-free extracts of
Streptomyces clavuligerus was investigated using α-ketoglutarate,
L-ascorbic acid, FeSO
4,
S-adenosyl-
L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with
Proteus vulgaris GN 76/C-1 and by high performance liquid chromatography. Although the conversion rate of 7α-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox.</description><subject>7-Methoxylation</subject><subject>a-Ketoglutaric acid</subject><subject>Ascorbic acid</subject><subject>Cefminox</subject><subject>Cell-free extract</subject><subject>Cephamycin</subject><subject>Proteus vulgaris GN 76/C-1</subject><subject>Streptomyces clavuligerus</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNo9kM1KAzEYRYMoWKuPIMxKdBH9ksnkZyUiaoWCi-o6zKRfNDKdqUmmtG9vf8TVvYvD5XIIuWRwy4DJuxmUSlMGRl0bcwMgQVF-REasUoJKI_UxGf0jp-QspW8AEBzkiExmmy5_YQqp6H3h0C9C16-LZrPtbUt9RCxwnWPt8p6Y5YjL3C82DlPh2no1tOET45DOyYmv24QXfzkmH89P748TOn17eX18mFLkWmWquWhq4bVjup77ShguUXNQjWe-Qo5VyQ0yWXnNjNFa-NrzxoMyZaNKI7Eck6vD7jL2PwOmbBch7b7WHfZDskwJZhSILXh_AHH7ZhUw2uQCdg7nIaLLdt4Hy8DuDNq9QbvTY42xe4OWl7_rL2VQ</recordid><startdate>20000115</startdate><enddate>20000115</enddate><creator>Kim, Jeong Keun</creator><creator>Kang, Heui-il</creator><creator>Chae, Jeong Seok</creator><creator>Park, Young-Hoon</creator><creator>Choi, Yong-Jin</creator><general>Elsevier B.V</general><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20000115</creationdate><title>Synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus</title><author>Kim, Jeong Keun ; Kang, Heui-il ; Chae, Jeong Seok ; Park, Young-Hoon ; Choi, Yong-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e287t-824ba4f8c18adf54926e8207bf1f5e2e5329e165f8199884faf2bf0793b7396e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>7-Methoxylation</topic><topic>a-Ketoglutaric acid</topic><topic>Ascorbic acid</topic><topic>Cefminox</topic><topic>Cell-free extract</topic><topic>Cephamycin</topic><topic>Proteus vulgaris GN 76/C-1</topic><topic>Streptomyces clavuligerus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Jeong Keun</creatorcontrib><creatorcontrib>Kang, Heui-il</creatorcontrib><creatorcontrib>Chae, Jeong Seok</creatorcontrib><creatorcontrib>Park, Young-Hoon</creatorcontrib><creatorcontrib>Choi, Yong-Jin</creatorcontrib><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Jeong Keun</au><au>Kang, Heui-il</au><au>Chae, Jeong Seok</au><au>Park, Young-Hoon</au><au>Choi, Yong-Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus</atitle><jtitle>FEMS microbiology letters</jtitle><date>2000-01-15</date><risdate>2000</risdate><volume>182</volume><issue>2</issue><spage>313</spage><epage>317</epage><pages>313-317</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>In vitro synthesis of cefminox by cell-free extracts of
Streptomyces clavuligerus was investigated using α-ketoglutarate,
L-ascorbic acid, FeSO
4,
S-adenosyl-
L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with
Proteus vulgaris GN 76/C-1 and by high performance liquid chromatography. Although the conversion rate of 7α-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S0378-1097(99)00607-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEMS microbiology letters, 2000-01, Vol.182 (2), p.313-317 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Wiley Online Library Journals; Oxford Academic Journals (OUP); Alma/SFX Local Collection |
| subjects | 7-Methoxylation a-Ketoglutaric acid Ascorbic acid Cefminox Cell-free extract Cephamycin Proteus vulgaris GN 76/C-1 Streptomyces clavuligerus |
| title | Synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus |
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