Biphasic effect of cadmium in non-cytotoxic conditions on the secretion of nitric oxide from peritoneal macrophages

The effects of cadmium (Cd) in non-cytotoxic conditions on the nitric oxide (NO) production in peritoneal macrophages (pM) were studied. Peritoneal macrophages from Balb/c mice were incubated over 18 h with 5, 10, 20, or 25 μM Cd 2+ (as CdCl 2 21:2 H 2O) in the culture medium. Concentrations of 20 μ...

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Veröffentlicht in:Toxicology (Amsterdam) 1999-11, Vol.139 (1), p.167-177
Hauptverfasser: Ramirez, Dario C., Martinez, Luis D., Marchevsky, Eduardo, Gimenez, Maria S.
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container_title Toxicology (Amsterdam)
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creator Ramirez, Dario C.
Martinez, Luis D.
Marchevsky, Eduardo
Gimenez, Maria S.
description The effects of cadmium (Cd) in non-cytotoxic conditions on the nitric oxide (NO) production in peritoneal macrophages (pM) were studied. Peritoneal macrophages from Balb/c mice were incubated over 18 h with 5, 10, 20, or 25 μM Cd 2+ (as CdCl 2 21:2 H 2O) in the culture medium. Concentrations of 20 μM Cd 2+ and over had cytotoxic effects, measured by MTT assay. Cell viability with 10 μM Cd 2+ in the medium was above 90% after 18 h of incubation, and above 80% after 72 h. At this same Cd 2+ concentration, NO production increased from 6 to 18 h. At 24 h production decreased but was still above control levels. At 48 h production NO was near control levels, and continued to decrease until the end of the experiment (72 h). NO levels produced with Cd 2+ concentrations of 5, 10 and 20 μM in the medium were above the control at 18 h. NO production and lipoperoxidation increased simultaneously after 18 h with 10 μM of Cd in the medium. Amounts of inducible nitric oxide synthase (iNOS) protein and iNOS activity also increased. At a concentration of 10 μM Cd has a biphasic effect on NO production over time.
doi_str_mv 10.1016/S0300-483X(99)00086-4
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Peritoneal macrophages from Balb/c mice were incubated over 18 h with 5, 10, 20, or 25 μM Cd 2+ (as CdCl 2 21:2 H 2O) in the culture medium. Concentrations of 20 μM Cd 2+ and over had cytotoxic effects, measured by MTT assay. Cell viability with 10 μM Cd 2+ in the medium was above 90% after 18 h of incubation, and above 80% after 72 h. At this same Cd 2+ concentration, NO production increased from 6 to 18 h. At 24 h production decreased but was still above control levels. At 48 h production NO was near control levels, and continued to decrease until the end of the experiment (72 h). NO levels produced with Cd 2+ concentrations of 5, 10 and 20 μM in the medium were above the control at 18 h. NO production and lipoperoxidation increased simultaneously after 18 h with 10 μM of Cd in the medium. Amounts of inducible nitric oxide synthase (iNOS) protein and iNOS activity also increased. 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Peritoneal macrophages from Balb/c mice were incubated over 18 h with 5, 10, 20, or 25 μM Cd 2+ (as CdCl 2 21:2 H 2O) in the culture medium. Concentrations of 20 μM Cd 2+ and over had cytotoxic effects, measured by MTT assay. Cell viability with 10 μM Cd 2+ in the medium was above 90% after 18 h of incubation, and above 80% after 72 h. At this same Cd 2+ concentration, NO production increased from 6 to 18 h. At 24 h production decreased but was still above control levels. At 48 h production NO was near control levels, and continued to decrease until the end of the experiment (72 h). NO levels produced with Cd 2+ concentrations of 5, 10 and 20 μM in the medium were above the control at 18 h. NO production and lipoperoxidation increased simultaneously after 18 h with 10 μM of Cd in the medium. Amounts of inducible nitric oxide synthase (iNOS) protein and iNOS activity also increased. 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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Animals
Biological and medical sciences
Blotting, Western
Cadmium
Cadmium - toxicity
Cell Separation
Cell Survival - drug effects
Chemical and industrial products toxicology. Toxic occupational diseases
Coloring Agents
In Vitro Techniques
Lipid Peroxidation - drug effects
Lipoperoxidation
Macrophages
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - metabolism
Male
Medical sciences
Metals and various inorganic compounds
Mice
Mice, Inbred BALB C
Nitric oxide
Nitric Oxide - metabolism
Nitric oxide synthase
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
peritoneum
Tetrazolium Salts
Thiazoles
Thiobarbituric Acid Reactive Substances - metabolism
Toxicology
title Biphasic effect of cadmium in non-cytotoxic conditions on the secretion of nitric oxide from peritoneal macrophages
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