FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiae and its benomyl‐induced expression is dependent on Pdr3 transcriptional regulator

In this work we report the disruption of a Saccharomyces cerevisiae ORF YBR008c (FLR1 gene) within the context of EUROFAN (EUROpean Functional Analysis Network) six‐pack programme, using a PCR‐mediated gene replacement protocol as well as the results of the basic phenotypic analysis of a deletant st...

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Veröffentlicht in:	Yeast (Chichester, England) England), 1999-11, Vol.15 (15), p.1595-1608
Hauptverfasser:	Brôco, Nuno, Tenreiro, Sandra, Viegas, Cristina A., Sá‐Correia, Isabel
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Nitroquinoline-1-oxide - pharmacology Antifungal Agents - pharmacology Antimetabolites, Antineoplastic - pharmacology benomyl Benomyl - pharmacology Carcinogens - pharmacology Carrier Proteins - genetics Cycloheximide - pharmacology DNA Primers - chemistry DNA, Fungal - chemistry DNA-Binding Proteins - genetics Drug Resistance, Microbial - genetics Electrophoresis, Agar Gel FLR1 gene Fluconazole - pharmacology Fungicides, Industrial - pharmacology Gene Expression Regulation, Fungal lacZ gene Membrane Transport Proteins methotrexate Methotrexate - pharmacology Mutagenesis, Insertional ORF YBR008c Organic Anion Transporters PDR1/PDR3 PDR3 gene Pdr3 protein Plasmids - chemistry Polymerase Chain Reaction Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Transcription Factors - genetics
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