Tracking Antigen-Specific CD8 T Lymphocytes in the Lungs of Mice Vaccinated with the Mtb72F Polyprotein
This study used a major histocompatibility complex class I tetramer reagent to track antigen-specific CD8 T cells in the lungs of mice immunized with the tuberculosis vaccine candidate Mtb72F. The results show that CD8 T cells recognizing an immunodominant Mtb32-specific epitope could be detected in...
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| Veröffentlicht in: | Infection and Immunity 2005-09, Vol.73 (9), p.5809-5816 |
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| creator | Irwin, Scott M Izzo, Angelo A Dow, Steven W Skeiky, Y. A. W Reed, Steven G Alderson, Mark R Orme, Ian M |
| description | This study used a major histocompatibility complex class I tetramer reagent to track antigen-specific CD8 T cells in the lungs of mice immunized with the tuberculosis vaccine candidate Mtb72F. The results show that CD8 T cells recognizing an immunodominant Mtb32-specific epitope could be detected in significant numbers over the course of infection in mice exposed to low-dose aerosol challenge with Mycobacterium tuberculosis and that prior vaccination substantially increased the numbers of these cells early in the lungs. The effector phenotype of the cells was shown by the demonstration that many secreted gamma interferon, but very few contained granzyme B. As the course of the infection progressed, many activated CD8 T cells down-regulated expression of CD45RB and upregulated expression of the interleukin-7 receptor alpha chain, indicating a transition of these cells to a state of memory. These data support the hypothesis that M. tuberculosis-specific CD8 T cells can be targeted by vaccination with the Mtb72F polyprotein. |
| doi_str_mv | 10.1128/IAI.73.9.5809-5816.2005 |
| format | Article |
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A. W ; Reed, Steven G ; Alderson, Mark R ; Orme, Ian M</creator><creatorcontrib>Irwin, Scott M ; Izzo, Angelo A ; Dow, Steven W ; Skeiky, Y. A. W ; Reed, Steven G ; Alderson, Mark R ; Orme, Ian M</creatorcontrib><description>This study used a major histocompatibility complex class I tetramer reagent to track antigen-specific CD8 T cells in the lungs of mice immunized with the tuberculosis vaccine candidate Mtb72F. The results show that CD8 T cells recognizing an immunodominant Mtb32-specific epitope could be detected in significant numbers over the course of infection in mice exposed to low-dose aerosol challenge with Mycobacterium tuberculosis and that prior vaccination substantially increased the numbers of these cells early in the lungs. The effector phenotype of the cells was shown by the demonstration that many secreted gamma interferon, but very few contained granzyme B. As the course of the infection progressed, many activated CD8 T cells down-regulated expression of CD45RB and upregulated expression of the interleukin-7 receptor alpha chain, indicating a transition of these cells to a state of memory. These data support the hypothesis that M. tuberculosis-specific CD8 T cells can be targeted by vaccination with the Mtb72F polyprotein.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.73.9.5809-5816.2005</identifier><identifier>PMID: 16113299</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Antigens, Bacterial - immunology ; Applied microbiology ; Bacterial Proteins - administration & dosage ; Bacterial Proteins - immunology ; BCG Vaccine - administration & dosage ; BCG Vaccine - immunology ; Biological and medical sciences ; CD8-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Granzymes ; Interferon-gamma - metabolism ; Kinetics ; Liposomes ; Lung - cytology ; Lung - immunology ; Lung - metabolism ; Mice ; Mice, Inbred C57BL ; Microbial Immunity and Vaccines ; Microbiology ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - immunology ; Receptors, Interleukin-7 - metabolism ; Serine Endopeptidases - metabolism ; Tuberculosis, Pulmonary - immunology ; Tuberculosis, Pulmonary - prevention & control ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>Infection and Immunity, 2005-09, Vol.73 (9), p.5809-5816</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-7c59622662cda4bdeaf424c7b31e5eb526c9198e543e7f585468117ded59b9b13</citedby><cites>FETCH-LOGICAL-c526t-7c59622662cda4bdeaf424c7b31e5eb526c9198e543e7f585468117ded59b9b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1231129/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1231129/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17037088$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16113299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Irwin, Scott M</creatorcontrib><creatorcontrib>Izzo, Angelo A</creatorcontrib><creatorcontrib>Dow, Steven W</creatorcontrib><creatorcontrib>Skeiky, Y. A. W</creatorcontrib><creatorcontrib>Reed, Steven G</creatorcontrib><creatorcontrib>Alderson, Mark R</creatorcontrib><creatorcontrib>Orme, Ian M</creatorcontrib><title>Tracking Antigen-Specific CD8 T Lymphocytes in the Lungs of Mice Vaccinated with the Mtb72F Polyprotein</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>This study used a major histocompatibility complex class I tetramer reagent to track antigen-specific CD8 T cells in the lungs of mice immunized with the tuberculosis vaccine candidate Mtb72F. The results show that CD8 T cells recognizing an immunodominant Mtb32-specific epitope could be detected in significant numbers over the course of infection in mice exposed to low-dose aerosol challenge with Mycobacterium tuberculosis and that prior vaccination substantially increased the numbers of these cells early in the lungs. The effector phenotype of the cells was shown by the demonstration that many secreted gamma interferon, but very few contained granzyme B. As the course of the infection progressed, many activated CD8 T cells down-regulated expression of CD45RB and upregulated expression of the interleukin-7 receptor alpha chain, indicating a transition of these cells to a state of memory. These data support the hypothesis that M. tuberculosis-specific CD8 T cells can be targeted by vaccination with the Mtb72F polyprotein.</description><subject>Animals</subject><subject>Antigens, Bacterial - immunology</subject><subject>Applied microbiology</subject><subject>Bacterial Proteins - administration & dosage</subject><subject>Bacterial Proteins - immunology</subject><subject>BCG Vaccine - administration & dosage</subject><subject>BCG Vaccine - immunology</subject><subject>Biological and medical sciences</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granzymes</subject><subject>Interferon-gamma - metabolism</subject><subject>Kinetics</subject><subject>Liposomes</subject><subject>Lung - cytology</subject><subject>Lung - immunology</subject><subject>Lung - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbial Immunity and Vaccines</subject><subject>Microbiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - immunology</subject><subject>Receptors, Interleukin-7 - metabolism</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Tuberculosis, Pulmonary - immunology</subject><subject>Tuberculosis, Pulmonary - prevention & control</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFu0zAUhiMEYt3gFZi5YHcptmPH9g1SVRhU6gTSOm4txzlJDKnT2SlT3x6HVhtcIVmyLH_n9_H5suyS4DkhVL5fLVZzUczVnEusci5JOacY82fZjGAlc84pfZ7NMCYqV7wUZ9l5jD_SkTEmX2ZnpCSkoErNsnYTjP3pfIsWfnQt-Px2B9Y1zqLlR4k2aH3Y7rrBHkaIyHk0doDWe99GNDToxllA3421zpsRavTgxu4PcTNWgl6jb0N_2IVhBOdfZS8a00d4fdovsrvrT5vll3z99fNquVjnltNyzIXlqqS0LKmtDatqMA2jzIqqIMChSoxVREngrADRcMlZKQkRNdRcVaoixUX24Zi721dbqC34MZhe74LbmnDQg3H63xvvOt0OvzShRZqsSgFXp4Aw3O8hjnrrooW-Nx6GfdSlZEoS-X-QCIYZp1NL4gjaMMQYoHnshmA92dTJphaFVnqyqSeberKZKt_8_ZmnupO-BLw7ASZa0zfBeOviEydwIbCUiXt75DrXdg8ugDZxq10axuOzibk8Mo0ZtGlDyrm7pZgUmGCaFi5-AxIyvKo</recordid><startdate>20050901</startdate><enddate>20050901</enddate><creator>Irwin, Scott M</creator><creator>Izzo, Angelo A</creator><creator>Dow, Steven W</creator><creator>Skeiky, Y. A. W</creator><creator>Reed, Steven G</creator><creator>Alderson, Mark R</creator><creator>Orme, Ian M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050901</creationdate><title>Tracking Antigen-Specific CD8 T Lymphocytes in the Lungs of Mice Vaccinated with the Mtb72F Polyprotein</title><author>Irwin, Scott M ; Izzo, Angelo A ; Dow, Steven W ; Skeiky, Y. A. W ; Reed, Steven G ; Alderson, Mark R ; Orme, Ian M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-7c59622662cda4bdeaf424c7b31e5eb526c9198e543e7f585468117ded59b9b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - immunology</topic><topic>Applied microbiology</topic><topic>Bacterial Proteins - administration & dosage</topic><topic>Bacterial Proteins - immunology</topic><topic>BCG Vaccine - administration & dosage</topic><topic>BCG Vaccine - immunology</topic><topic>Biological and medical sciences</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granzymes</topic><topic>Interferon-gamma - metabolism</topic><topic>Kinetics</topic><topic>Liposomes</topic><topic>Lung - cytology</topic><topic>Lung - immunology</topic><topic>Lung - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbial Immunity and Vaccines</topic><topic>Microbiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - immunology</topic><topic>Receptors, Interleukin-7 - metabolism</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Tuberculosis, Pulmonary - immunology</topic><topic>Tuberculosis, Pulmonary - prevention & control</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Irwin, Scott M</creatorcontrib><creatorcontrib>Izzo, Angelo A</creatorcontrib><creatorcontrib>Dow, Steven W</creatorcontrib><creatorcontrib>Skeiky, Y. 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W</creatorcontrib><creatorcontrib>Reed, Steven G</creatorcontrib><creatorcontrib>Alderson, Mark R</creatorcontrib><creatorcontrib>Orme, Ian M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Irwin, Scott M</au><au>Izzo, Angelo A</au><au>Dow, Steven W</au><au>Skeiky, Y. A. W</au><au>Reed, Steven G</au><au>Alderson, Mark R</au><au>Orme, Ian M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tracking Antigen-Specific CD8 T Lymphocytes in the Lungs of Mice Vaccinated with the Mtb72F Polyprotein</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>2005-09-01</date><risdate>2005</risdate><volume>73</volume><issue>9</issue><spage>5809</spage><epage>5816</epage><pages>5809-5816</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>This study used a major histocompatibility complex class I tetramer reagent to track antigen-specific CD8 T cells in the lungs of mice immunized with the tuberculosis vaccine candidate Mtb72F. The results show that CD8 T cells recognizing an immunodominant Mtb32-specific epitope could be detected in significant numbers over the course of infection in mice exposed to low-dose aerosol challenge with Mycobacterium tuberculosis and that prior vaccination substantially increased the numbers of these cells early in the lungs. The effector phenotype of the cells was shown by the demonstration that many secreted gamma interferon, but very few contained granzyme B. As the course of the infection progressed, many activated CD8 T cells down-regulated expression of CD45RB and upregulated expression of the interleukin-7 receptor alpha chain, indicating a transition of these cells to a state of memory. These data support the hypothesis that M. tuberculosis-specific CD8 T cells can be targeted by vaccination with the Mtb72F polyprotein.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16113299</pmid><doi>10.1128/IAI.73.9.5809-5816.2005</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Antigens, Bacterial - immunology Applied microbiology Bacterial Proteins - administration & dosage Bacterial Proteins - immunology BCG Vaccine - administration & dosage BCG Vaccine - immunology Biological and medical sciences CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism Female Fundamental and applied biological sciences. Psychology Granzymes Interferon-gamma - metabolism Kinetics Liposomes Lung - cytology Lung - immunology Lung - metabolism Mice Mice, Inbred C57BL Microbial Immunity and Vaccines Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - immunology Receptors, Interleukin-7 - metabolism Serine Endopeptidases - metabolism Tuberculosis, Pulmonary - immunology Tuberculosis, Pulmonary - prevention & control Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Tracking Antigen-Specific CD8 T Lymphocytes in the Lungs of Mice Vaccinated with the Mtb72F Polyprotein |
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