Phytol directly activates peroxisome proliferator-activated receptor α (PPARα) and regulates gene expression involved in lipid metabolism in PPARα-expressing HepG2 hepatocytes
The peroxisome proliferator-activated receptor (PPAR) is one of the indispensable transcription factors for regulating lipid metabolism in various tissues. In our screening for natural compounds that activate PPAR using luciferase assays, a branched-carbon-chain alcohol (a component of chlorophylls)...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-11, Vol.337 (2), p.440-445 |
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| container_end_page | 445 |
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| container_issue | 2 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 337 |
| creator | Goto, Tsuyoshi Takahashi, Nobuyuki Kato, Sota Egawa, Kahori Ebisu, Shogo Moriyama, Tatsuya Fushiki, Tohru Kawada, Teruo |
| description | The peroxisome proliferator-activated receptor (PPAR) is one of the indispensable transcription factors for regulating lipid metabolism in various tissues. In our screening for natural compounds that activate PPAR using luciferase assays, a branched-carbon-chain alcohol (a component of chlorophylls), phytol, has been identified as a PPARα-specific activator. Phytol induced the increase in PPARα-dependent luciferase activity and the degree of in vitro binding of a coactivator, SRC-1, to GST-PPARα. Moreover, the addition of phytol upregulated the expression of PPARα-target genes at both mRNA and protein levels in PPARα-expressing HepG2 hepatocytes. These findings indicate that phytol is functional as a PPARα ligand and that it stimulates the expression of PPARα-target genes in intact cells. Because PPARα activation enhances circulating lipid clearance, phytol may be important in managing abnormalities in lipid metabolism. |
| doi_str_mv | 10.1016/j.bbrc.2005.09.077 |
| format | Article |
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| ispartof | Biochemical and biophysical research communications, 2005-11, Vol.337 (2), p.440-445 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Fibrate Hepatocytes Phytanic acid Phytol PPARα |
| title | Phytol directly activates peroxisome proliferator-activated receptor α (PPARα) and regulates gene expression involved in lipid metabolism in PPARα-expressing HepG2 hepatocytes |
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