Immunological detection of syringopeptins produced by Pseudomonas syringae pv. lachrymans
Several strains of plant pathogenic Pseudomonas are known to produce phytotoxic lipodepsipeptides (syringomycin, syringopeptins and related compounds) in vitro. However, detection of these compounds in organic extracts from diseased plant tissues has been attempted by chromatographic methods for syr...
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| Veröffentlicht in: | Physiological and molecular plant pathology 1999-11, Vol.55 (5), p.255-261 |
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| container_title | Physiological and molecular plant pathology |
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| creator | FOGLIANO, V GALLO, M VINALE, F RITIENI, A RANDAZZO, G GRECO, M LOPS, R GRANITI, A |
| description | Several strains of plant pathogenic Pseudomonas are known to produce phytotoxic lipodepsipeptides (syringomycin, syringopeptins and related compounds) in vitro. However, detection of these compounds in organic extracts from diseased plant tissues has been attempted by chromatographic methods for syringomycin only. A macromolecular derivative of syringopeptins (KLH-SP25A+B) was used to raise polyclonal antibodies in rabbit. The antiserum was able to recognize free syringopeptins (SP22and SP25) with an estimated detection limit of 0.05 μg per well in the indirect ELISA, and 0.01 μg per well in the competitive ELISA. Cross-reaction with other structurally related lipodepsipeptides, e.g. syringomycins and pseudomycin A, was less than 20%. Using the competitive ELISA method developed in this study, relatively abundant quantities of the toxins, i.e. approximately 0.1 mg of syringopeptins per gram fresh tissue, were detected when an aqueous extract from cotyledons of zucchini infected with a strain of P. syringae pv. lachrymans was tested. |
| doi_str_mv | 10.1006/pmpp.1999.0227 |
| format | Article |
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Using the competitive ELISA method developed in this study, relatively abundant quantities of the toxins, i.e. approximately 0.1 mg of syringopeptins per gram fresh tissue, were detected when an aqueous extract from cotyledons of zucchini infected with a strain of P. syringae pv. lachrymans was tested.</description><identifier>ISSN: 0885-5765</identifier><identifier>EISSN: 1096-1178</identifier><identifier>DOI: 10.1006/pmpp.1999.0227</identifier><identifier>CODEN: PMPPEZ</identifier><language>eng</language><publisher>London: Elsevier India Pvt Ltd</publisher><subject>Bacterial plant pathogens ; Biological and medical sciences ; Cucurbita pepo ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control ; Phytopathology. Animal pests. 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Using the competitive ELISA method developed in this study, relatively abundant quantities of the toxins, i.e. approximately 0.1 mg of syringopeptins per gram fresh tissue, were detected when an aqueous extract from cotyledons of zucchini infected with a strain of P. syringae pv. lachrymans was tested.</description><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>Cucurbita pepo</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</subject><subject>Phytopathology. Animal pests. 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However, detection of these compounds in organic extracts from diseased plant tissues has been attempted by chromatographic methods for syringomycin only. A macromolecular derivative of syringopeptins (KLH-SP25A+B) was used to raise polyclonal antibodies in rabbit. The antiserum was able to recognize free syringopeptins (SP22and SP25) with an estimated detection limit of 0.05 μg per well in the indirect ELISA, and 0.01 μg per well in the competitive ELISA. Cross-reaction with other structurally related lipodepsipeptides, e.g. syringomycins and pseudomycin A, was less than 20%. 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| subjects | Bacterial plant pathogens Biological and medical sciences Cucurbita pepo Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control Phytopathology. Animal pests. Plant and forest protection Pseudomonas syringae lachrymans Pseudomonas syringae pv. lachrymans syringopeptins zucchini |
| title | Immunological detection of syringopeptins produced by Pseudomonas syringae pv. lachrymans |
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