Recurrent alterations of the short arm of chromosome 3 define a tumor suppressor region in rat mammary tumor cells
Cytogenetic alterations associated with different stages in carcinogenesis can be distinguished in cultured human or rodent cells transformed by carcinogenic agents. Three tumorigenic rat mammary epithelial cell lines transformed in vitro with 7,12,-dimethylbenz[a]anthracene alone or in combination...
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| Veröffentlicht in: | Carcinogenesis (New York) 1999-10, Vol.20 (10), p.2033-2036 |
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| creator | Popescu, Nicholas C. Greiner, John W. |
| description | Cytogenetic alterations associated with different stages in carcinogenesis can be distinguished in cultured human or rodent cells transformed by carcinogenic agents. Three tumorigenic rat mammary epithelial cell lines transformed in vitro with 7,12,-dimethylbenz[a]anthracene alone or in combination with 12-O-tetradecanoylphorbol-13-acetate were examined cytogenetically. Non-random alterations consisting of translocations involving the short arm of chromosome 3 and trisomy of chromosomes 14 and X were identified in all three lines. Deletion and inversion of chromosome 1 with the breakpoint at band 1q22 and a duplication 1q 32–43 and trisomy of chromosome 2 were observed in two cell lines. The accumulation of structural alterations and chromosome imbalances during the process of cell immortalization and acquisition of tumorigenicity are required for normal rat mammary cells to become malignant. Unbalanced translocations of chromosome 3 resulting in loss of the short arm had the breakpoint at 3p11. This site is a hotspot of breakage and recombination in various rat tumors and may represent a region of tumor suppressor gene critical to the development of rat mammary tumors, as well as other types of tumors. |
| doi_str_mv | 10.1093/carcin/20.10.2033 |
| format | Article |
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Three tumorigenic rat mammary epithelial cell lines transformed in vitro with 7,12,-dimethylbenz[a]anthracene alone or in combination with 12-O-tetradecanoylphorbol-13-acetate were examined cytogenetically. Non-random alterations consisting of translocations involving the short arm of chromosome 3 and trisomy of chromosomes 14 and X were identified in all three lines. Deletion and inversion of chromosome 1 with the breakpoint at band 1q22 and a duplication 1q 32–43 and trisomy of chromosome 2 were observed in two cell lines. The accumulation of structural alterations and chromosome imbalances during the process of cell immortalization and acquisition of tumorigenicity are required for normal rat mammary cells to become malignant. Unbalanced translocations of chromosome 3 resulting in loss of the short arm had the breakpoint at 3p11. This site is a hotspot of breakage and recombination in various rat tumors and may represent a region of tumor suppressor gene critical to the development of rat mammary tumors, as well as other types of tumors.</description><identifier>ISSN: 0143-3334</identifier><identifier>EISSN: 1460-2180</identifier><identifier>DOI: 10.1093/carcin/20.10.2033</identifier><identifier>PMID: 10506121</identifier><identifier>CODEN: CRNGDP</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>12-dimethylbenz[a]anthracene ; 12-O-tetradecanoylphorbol-13-acetate ; 7,12-dimethylbenz(a)anthracene ; Animal tumors. Experimental tumors ; Animals ; Biological and medical sciences ; Cell Line, Transformed ; chromosome 1 ; chromosome 14 ; chromosome 2 ; chromosome 3 ; Chromosome Aberrations ; DMBA ; Experimental genital and mammary tumors ; FBS ; Female ; fetal bovine serum ; Genes, Tumor Suppressor ; Humans ; Karyotyping ; LOH ; loss of heterozygosity ; Mammary Neoplasms, Experimental - chemically induced ; Mammary Neoplasms, Experimental - genetics ; Medical sciences ; N-nitroso-N-methylurea ; NMU ; NOR ; nucleolar organizer regions ; Rats ; Rats, Sprague-Dawley ; TPA ; Tumor Cells, Cultured ; Tumors</subject><ispartof>Carcinogenesis (New York), 1999-10, Vol.20 (10), p.2033-2036</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Oct 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-6ea114a31e78b6c679102e3bec26a2d3532bda26535b1238fb1c178c28ea416c3</citedby><cites>FETCH-LOGICAL-c465t-6ea114a31e78b6c679102e3bec26a2d3532bda26535b1238fb1c178c28ea416c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1978498$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10506121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Popescu, Nicholas C.</creatorcontrib><creatorcontrib>Greiner, John W.</creatorcontrib><title>Recurrent alterations of the short arm of chromosome 3 define a tumor suppressor region in rat mammary tumor cells</title><title>Carcinogenesis (New York)</title><addtitle>Carcinogenesis</addtitle><description>Cytogenetic alterations associated with different stages in carcinogenesis can be distinguished in cultured human or rodent cells transformed by carcinogenic agents. Three tumorigenic rat mammary epithelial cell lines transformed in vitro with 7,12,-dimethylbenz[a]anthracene alone or in combination with 12-O-tetradecanoylphorbol-13-acetate were examined cytogenetically. Non-random alterations consisting of translocations involving the short arm of chromosome 3 and trisomy of chromosomes 14 and X were identified in all three lines. Deletion and inversion of chromosome 1 with the breakpoint at band 1q22 and a duplication 1q 32–43 and trisomy of chromosome 2 were observed in two cell lines. The accumulation of structural alterations and chromosome imbalances during the process of cell immortalization and acquisition of tumorigenicity are required for normal rat mammary cells to become malignant. Unbalanced translocations of chromosome 3 resulting in loss of the short arm had the breakpoint at 3p11. This site is a hotspot of breakage and recombination in various rat tumors and may represent a region of tumor suppressor gene critical to the development of rat mammary tumors, as well as other types of tumors.</description><subject>12-dimethylbenz[a]anthracene</subject><subject>12-O-tetradecanoylphorbol-13-acetate</subject><subject>7,12-dimethylbenz(a)anthracene</subject><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Transformed</subject><subject>chromosome 1</subject><subject>chromosome 14</subject><subject>chromosome 2</subject><subject>chromosome 3</subject><subject>Chromosome Aberrations</subject><subject>DMBA</subject><subject>Experimental genital and mammary tumors</subject><subject>FBS</subject><subject>Female</subject><subject>fetal bovine serum</subject><subject>Genes, Tumor Suppressor</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>LOH</subject><subject>loss of heterozygosity</subject><subject>Mammary Neoplasms, Experimental - chemically induced</subject><subject>Mammary Neoplasms, Experimental - genetics</subject><subject>Medical sciences</subject><subject>N-nitroso-N-methylurea</subject><subject>NMU</subject><subject>NOR</subject><subject>nucleolar organizer regions</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>TPA</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0143-3334</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1v1DAQhi0EokvhB3BBFkLc0no8iZMc0QooUlGlFqSqF8vxTtiUJF7GiVT-PY6yAtTTfD3zauxXiNegzkDVeO4d-24810t5phXiE7GB3KhMQ6Weio2CHDNEzE_EixjvlQKDRf1cnIAqlAENG8HX5GdmGifp-onYTV0YowytnPYk4z5wGvCwNPyewxBiGEii3FHbjSSdnOYhsIzz4cAUY0qZfiQJ2Y0yicnBDYPj30fMU9_Hl-JZ6_pIr47xVHz_9PHb9iK7vPr8ZfvhMvO5KabMkAPIHQKVVWO8KWtQmrAhr43TOyxQNzunTYFFAxqrtgEPZeV1RS4H4_FUvF91Dxx-zRQnO3RxucCNFOZoocS6MLVJ4NtH4H2YeUy3WQ01Qm2MThCskOcQI1NrD9wtT7Og7OKGXd2weint4kbaeXMUnpuBdv9trN-fgHdHwEXv-pbd6Lv4j6vLKq-rhGUr1sWJHv6OHf-0psSysBe3d_buK2zL69sbu8U_vi6jaQ</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Popescu, Nicholas C.</creator><creator>Greiner, John W.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19991001</creationdate><title>Recurrent alterations of the short arm of chromosome 3 define a tumor suppressor region in rat mammary tumor cells</title><author>Popescu, Nicholas C. ; Greiner, John W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-6ea114a31e78b6c679102e3bec26a2d3532bda26535b1238fb1c178c28ea416c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>12-dimethylbenz[a]anthracene</topic><topic>12-O-tetradecanoylphorbol-13-acetate</topic><topic>7,12-dimethylbenz(a)anthracene</topic><topic>Animal tumors. Experimental tumors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Transformed</topic><topic>chromosome 1</topic><topic>chromosome 14</topic><topic>chromosome 2</topic><topic>chromosome 3</topic><topic>Chromosome Aberrations</topic><topic>DMBA</topic><topic>Experimental genital and mammary tumors</topic><topic>FBS</topic><topic>Female</topic><topic>fetal bovine serum</topic><topic>Genes, Tumor Suppressor</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>LOH</topic><topic>loss of heterozygosity</topic><topic>Mammary Neoplasms, Experimental - chemically induced</topic><topic>Mammary Neoplasms, Experimental - genetics</topic><topic>Medical sciences</topic><topic>N-nitroso-N-methylurea</topic><topic>NMU</topic><topic>NOR</topic><topic>nucleolar organizer regions</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>TPA</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Popescu, Nicholas C.</creatorcontrib><creatorcontrib>Greiner, John W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Carcinogenesis (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Popescu, Nicholas C.</au><au>Greiner, John W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recurrent alterations of the short arm of chromosome 3 define a tumor suppressor region in rat mammary tumor cells</atitle><jtitle>Carcinogenesis (New York)</jtitle><addtitle>Carcinogenesis</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>20</volume><issue>10</issue><spage>2033</spage><epage>2036</epage><pages>2033-2036</pages><issn>0143-3334</issn><eissn>1460-2180</eissn><coden>CRNGDP</coden><abstract>Cytogenetic alterations associated with different stages in carcinogenesis can be distinguished in cultured human or rodent cells transformed by carcinogenic agents. Three tumorigenic rat mammary epithelial cell lines transformed in vitro with 7,12,-dimethylbenz[a]anthracene alone or in combination with 12-O-tetradecanoylphorbol-13-acetate were examined cytogenetically. Non-random alterations consisting of translocations involving the short arm of chromosome 3 and trisomy of chromosomes 14 and X were identified in all three lines. Deletion and inversion of chromosome 1 with the breakpoint at band 1q22 and a duplication 1q 32–43 and trisomy of chromosome 2 were observed in two cell lines. The accumulation of structural alterations and chromosome imbalances during the process of cell immortalization and acquisition of tumorigenicity are required for normal rat mammary cells to become malignant. Unbalanced translocations of chromosome 3 resulting in loss of the short arm had the breakpoint at 3p11. This site is a hotspot of breakage and recombination in various rat tumors and may represent a region of tumor suppressor gene critical to the development of rat mammary tumors, as well as other types of tumors.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>10506121</pmid><doi>10.1093/carcin/20.10.2033</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 12-dimethylbenz[a]anthracene 12-O-tetradecanoylphorbol-13-acetate 7,12-dimethylbenz(a)anthracene Animal tumors. Experimental tumors Animals Biological and medical sciences Cell Line, Transformed chromosome 1 chromosome 14 chromosome 2 chromosome 3 Chromosome Aberrations DMBA Experimental genital and mammary tumors FBS Female fetal bovine serum Genes, Tumor Suppressor Humans Karyotyping LOH loss of heterozygosity Mammary Neoplasms, Experimental - chemically induced Mammary Neoplasms, Experimental - genetics Medical sciences N-nitroso-N-methylurea NMU NOR nucleolar organizer regions Rats Rats, Sprague-Dawley TPA Tumor Cells, Cultured Tumors |
| title | Recurrent alterations of the short arm of chromosome 3 define a tumor suppressor region in rat mammary tumor cells |
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