Elucidating the Role of H/ACA-like RNAs in trans-Splicing and rRNA Processing via RNA Interference Silencing of the Trypanosoma brucei CBF5 Pseudouridine Synthase

Most pseudouridinylation in eukaryotic rRNA and small nuclear RNAs is guided by H/ACA small nucleolar RNAs. In this study, the Trypanosoma brucei pseudouridine synthase, Cbf5p, a snoRNP protein, was identified and silenced by RNAi. Depletion of this protein destabilized all small nucleolar RNAs of t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 2005-10, Vol.280 (41), p.34558-34568
Hauptverfasser:	Barth, Sarit, Hury, Avraham, Liang, Xue-hai, Michaeli, Shulamit
Format:	Artikel
Sprache:	eng
Schlagworte:	Alternative Splicing Animals Base Sequence Blotting, Northern Gene Deletion Gene Silencing Hydro-Lyases - chemistry Hydro-Lyases - genetics Hydro-Lyases - metabolism Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism Models, Genetic Molecular Sequence Data Oligonucleotides - chemistry Oligonucleotides - genetics Phenotype Pseudouridine - chemistry Ribonucleoproteins, Small Nuclear - genetics Ribonucleoproteins, Small Nuclear - metabolism Ribonucleoproteins, Small Nucleolar - genetics RNA - metabolism RNA Interference RNA Splicing RNA, Messenger - metabolism RNA, Ribosomal - chemistry RNA, Ribosomal - metabolism RNA, Small Nuclear - metabolism RNA, Small Nucleolar - chemistry RNA, Small Nucleolar - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Temperature Time Factors Transfection Trypanosoma - metabolism Trypanosoma brucei Trypanosoma brucei brucei - enzymology Trypanosoma brucei brucei - genetics Tubulin - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!