Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants

Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants

Background The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA)...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of the Pediatric Infectious Diseases Society 2013-03, Vol.2 (1), p.30-39
Hauptverfasser: Suryadevara, Manika, Clark, Andrew E., Wolk, Donna M., Carman, Aubri, Rosenbaum, Paula F., Shaw, Jana
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 39
container_issue 1
container_start_page 30
container_title Journal of the Pediatric Infectious Diseases Society
container_volume 2
creator Suryadevara, Manika
Clark, Andrew E.
Wolk, Donna M.
Carman, Aubri
Rosenbaum, Paula F.
Shaw, Jana
description Background The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA) isolates from children in the Central New York (CNY) area and the prevalence of selected virulence genes. Methods Clinical and demographic information for hospitalized children
doi_str_mv 10.1093/jpids/pis087
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1738816787</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/jpids/pis087</oup_id><sourcerecordid>1738816787</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-5ffa540deef1a19e557a933f1674c63ef5da9653578924af09339eccdd0ee7b33</originalsourceid><addsrcrecordid>eNp9kU1P3DAQhqOqFSDKjXPlW3voFnsdx0lvVdrCSvRD4kPqKRrssTB17GA7IPh3_Wc1u0uPncuMRo8ejeatqkNGPzDa8aObyep0NNlEW_mi2lvSul3IJZUvn2fW8d3qIKUbWqoRTLT1TrW7bBrW1TXdq_58Cw7V7CCS_hoiqIzRPkK2wZNgyMrfQbJ3SM4yTNcPLqig1JwIzBFLW3mDas1aT3r0OYIj3_Ge_ArxdxFapyP6j2Q1TiFm8AqfpOf3gfQu-MIexzBPRed1cangk025aMjPiAm3-BmWCzNqcmnj7Nbbz1jOHK0Hn9Pr6pUBl_Bg2_eri69fzvuTxemP41X_6XSheMPyQhgDoqYa0TBgHQohoePcsEbWquFohIauEVzItlvWYMp3eYdKaU0R5RXn-9W7jXeK4XbGlIfRJoXOgccwp4FJ3rbF1sqCvt-gKoaUIpphinaE-DAwOjzlNqxzGza5FfzN1jxfjaj_wc8pFeDtBijP-r_qLwDTp_0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1738816787</pqid></control><display><type>article</type><title>Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Suryadevara, Manika ; Clark, Andrew E. ; Wolk, Donna M. ; Carman, Aubri ; Rosenbaum, Paula F. ; Shaw, Jana</creator><creatorcontrib>Suryadevara, Manika ; Clark, Andrew E. ; Wolk, Donna M. ; Carman, Aubri ; Rosenbaum, Paula F. ; Shaw, Jana</creatorcontrib><description>Background The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA) isolates from children in the Central New York (CNY) area and the prevalence of selected virulence genes. Methods Clinical and demographic information for hospitalized children &lt;19 years of age with community-onset or community-associated ISA infections, determined from clinical microbiology records, was extracted from medical records from Upstate Golisano Children's Hospital in CNY. Antibiotic susceptibility was assessed, and available isolates were genotyped and tested for the presence of selected virulence determinants. Associations between clinical and laboratory findings were evaluated using standard statistical techniques. Results Ninety patients with ISA disease diagnosed between 2007 and 2010 were included in the study; 74% were due to methicillin-susceptible SA (MSSA). The most common clinical diagnosis was bacteremia. Fifty-seven of 90 isolates were available for further testing. The SA pulsed-field gel electrophoresis type, agr type, and clonal complexes most commonly isolated were USA300 (n = 25, 44%), agr1 (n = 30, 52%), and CC8 (n = 25, 44%), respectively. USA300 strains were more likely to be associated with deep abscesses (P = .007), whereas non-USA300 strains were associated with medical device infections (P = .018). Isolates from patients with deep abscesses and pneumonia were more likely to carry luk-PV genes (P = .023 and P = .051, respectively). Conclusions MSSA remains an important problem of pediatric ISA infection in our region and results from genetically diverse SA populations.</description><identifier>ISSN: 2048-7193</identifier><identifier>EISSN: 2048-7207</identifier><identifier>DOI: 10.1093/jpids/pis087</identifier><identifier>PMID: 26619440</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><ispartof>Journal of the Pediatric Infectious Diseases Society, 2013-03, Vol.2 (1), p.30-39</ispartof><rights>The Author 2012. Published by Oxford University Press on behalf of the Pediatric Infectious Diseases Society. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com. 2012</rights><rights>The Author 2012. Published by Oxford University Press on behalf of the Pediatric Infectious Diseases Society. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-5ffa540deef1a19e557a933f1674c63ef5da9653578924af09339eccdd0ee7b33</citedby><cites>FETCH-LOGICAL-c361t-5ffa540deef1a19e557a933f1674c63ef5da9653578924af09339eccdd0ee7b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1585,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26619440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suryadevara, Manika</creatorcontrib><creatorcontrib>Clark, Andrew E.</creatorcontrib><creatorcontrib>Wolk, Donna M.</creatorcontrib><creatorcontrib>Carman, Aubri</creatorcontrib><creatorcontrib>Rosenbaum, Paula F.</creatorcontrib><creatorcontrib>Shaw, Jana</creatorcontrib><title>Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants</title><title>Journal of the Pediatric Infectious Diseases Society</title><addtitle>J Pediatric Infect Dis Soc</addtitle><description>Background The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA) isolates from children in the Central New York (CNY) area and the prevalence of selected virulence genes. Methods Clinical and demographic information for hospitalized children &lt;19 years of age with community-onset or community-associated ISA infections, determined from clinical microbiology records, was extracted from medical records from Upstate Golisano Children's Hospital in CNY. Antibiotic susceptibility was assessed, and available isolates were genotyped and tested for the presence of selected virulence determinants. Associations between clinical and laboratory findings were evaluated using standard statistical techniques. Results Ninety patients with ISA disease diagnosed between 2007 and 2010 were included in the study; 74% were due to methicillin-susceptible SA (MSSA). The most common clinical diagnosis was bacteremia. Fifty-seven of 90 isolates were available for further testing. The SA pulsed-field gel electrophoresis type, agr type, and clonal complexes most commonly isolated were USA300 (n = 25, 44%), agr1 (n = 30, 52%), and CC8 (n = 25, 44%), respectively. USA300 strains were more likely to be associated with deep abscesses (P = .007), whereas non-USA300 strains were associated with medical device infections (P = .018). Isolates from patients with deep abscesses and pneumonia were more likely to carry luk-PV genes (P = .023 and P = .051, respectively). Conclusions MSSA remains an important problem of pediatric ISA infection in our region and results from genetically diverse SA populations.</description><issn>2048-7193</issn><issn>2048-7207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kU1P3DAQhqOqFSDKjXPlW3voFnsdx0lvVdrCSvRD4kPqKRrssTB17GA7IPh3_Wc1u0uPncuMRo8ejeatqkNGPzDa8aObyep0NNlEW_mi2lvSul3IJZUvn2fW8d3qIKUbWqoRTLT1TrW7bBrW1TXdq_58Cw7V7CCS_hoiqIzRPkK2wZNgyMrfQbJ3SM4yTNcPLqig1JwIzBFLW3mDas1aT3r0OYIj3_Ge_ArxdxFapyP6j2Q1TiFm8AqfpOf3gfQu-MIexzBPRed1cangk025aMjPiAm3-BmWCzNqcmnj7Nbbz1jOHK0Hn9Pr6pUBl_Bg2_eri69fzvuTxemP41X_6XSheMPyQhgDoqYa0TBgHQohoePcsEbWquFohIauEVzItlvWYMp3eYdKaU0R5RXn-9W7jXeK4XbGlIfRJoXOgccwp4FJ3rbF1sqCvt-gKoaUIpphinaE-DAwOjzlNqxzGza5FfzN1jxfjaj_wc8pFeDtBijP-r_qLwDTp_0</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Suryadevara, Manika</creator><creator>Clark, Andrew E.</creator><creator>Wolk, Donna M.</creator><creator>Carman, Aubri</creator><creator>Rosenbaum, Paula F.</creator><creator>Shaw, Jana</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants</title><author>Suryadevara, Manika ; Clark, Andrew E. ; Wolk, Donna M. ; Carman, Aubri ; Rosenbaum, Paula F. ; Shaw, Jana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-5ffa540deef1a19e557a933f1674c63ef5da9653578924af09339eccdd0ee7b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Suryadevara, Manika</creatorcontrib><creatorcontrib>Clark, Andrew E.</creatorcontrib><creatorcontrib>Wolk, Donna M.</creatorcontrib><creatorcontrib>Carman, Aubri</creatorcontrib><creatorcontrib>Rosenbaum, Paula F.</creatorcontrib><creatorcontrib>Shaw, Jana</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the Pediatric Infectious Diseases Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suryadevara, Manika</au><au>Clark, Andrew E.</au><au>Wolk, Donna M.</au><au>Carman, Aubri</au><au>Rosenbaum, Paula F.</au><au>Shaw, Jana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants</atitle><jtitle>Journal of the Pediatric Infectious Diseases Society</jtitle><addtitle>J Pediatric Infect Dis Soc</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>2</volume><issue>1</issue><spage>30</spage><epage>39</epage><pages>30-39</pages><issn>2048-7193</issn><eissn>2048-7207</eissn><abstract>Background The genetic makeup of circulating Staphylococcus aureus (SA) populations varies by region. The extent to which SA virulence determinants contribute to the severity of pediatric infections is poorly understood. The study objective was to describe the genetic population of invasive SA (ISA) isolates from children in the Central New York (CNY) area and the prevalence of selected virulence genes. Methods Clinical and demographic information for hospitalized children &lt;19 years of age with community-onset or community-associated ISA infections, determined from clinical microbiology records, was extracted from medical records from Upstate Golisano Children's Hospital in CNY. Antibiotic susceptibility was assessed, and available isolates were genotyped and tested for the presence of selected virulence determinants. Associations between clinical and laboratory findings were evaluated using standard statistical techniques. Results Ninety patients with ISA disease diagnosed between 2007 and 2010 were included in the study; 74% were due to methicillin-susceptible SA (MSSA). The most common clinical diagnosis was bacteremia. Fifty-seven of 90 isolates were available for further testing. The SA pulsed-field gel electrophoresis type, agr type, and clonal complexes most commonly isolated were USA300 (n = 25, 44%), agr1 (n = 30, 52%), and CC8 (n = 25, 44%), respectively. USA300 strains were more likely to be associated with deep abscesses (P = .007), whereas non-USA300 strains were associated with medical device infections (P = .018). Isolates from patients with deep abscesses and pneumonia were more likely to carry luk-PV genes (P = .023 and P = .051, respectively). Conclusions MSSA remains an important problem of pediatric ISA infection in our region and results from genetically diverse SA populations.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>26619440</pmid><doi>10.1093/jpids/pis087</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2048-7193
ispartof Journal of the Pediatric Infectious Diseases Society, 2013-03, Vol.2 (1), p.30-39
issn 2048-7193
2048-7207
language eng
recordid cdi_proquest_miscellaneous_1738816787
source Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection
title Molecular Characterization of Invasive Staphylococcus aureus Infection in Central New York Children: Importance of Two Clonal Groups and Inconsistent Presence of Selected Virulence Determinants
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T11%3A16%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Characterization%20of%20Invasive%20Staphylococcus%20aureus%20Infection%20in%20Central%20New%20York%20Children:%20Importance%20of%20Two%20Clonal%20Groups%20and%20Inconsistent%20Presence%20of%20Selected%20Virulence%20Determinants&rft.jtitle=Journal%20of%20the%20Pediatric%20Infectious%20Diseases%20Society&rft.au=Suryadevara,%20Manika&rft.date=2013-03-01&rft.volume=2&rft.issue=1&rft.spage=30&rft.epage=39&rft.pages=30-39&rft.issn=2048-7193&rft.eissn=2048-7207&rft_id=info:doi/10.1093/jpids/pis087&rft_dat=%3Cproquest_cross%3E1738816787%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1738816787&rft_id=info:pmid/26619440&rft_oup_id=10.1093/jpids/pis087&rfr_iscdi=true