Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model
Rheumatoid arthritis (RA) is one of several inflammatory and autoimmune diseases that affect approximately 1 % of world’s population. The development of TNF inhibitors in the last decade represents a great advance in the treatment of mild and severe forms of RA. Etanercept is one of these drugs that...
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| Veröffentlicht in: | Naunyn-Schmiedeberg's archives of pharmacology 2015-12, Vol.388 (12), p.1247-1257 |
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| creator | Mattei, Rodrigo Antônio Dalmarco, Eduardo Monguilhott Fröde, Tânia Silvia |
| description | Rheumatoid arthritis (RA) is one of several inflammatory and autoimmune diseases that affect approximately 1 % of world’s population. The development of TNF inhibitors in the last decade represents a great advance in the treatment of mild and severe forms of RA. Etanercept is one of these drugs that is useful for RA treatment, but the mechanism of inhibition of the signaling pathway of inflammation was not completely elucidated. This study was conducted to evaluate the anti-inflammatory effect of etanercept in comparison to reference drugs (dexamethasone and indomethacin). Inflammation was induced by subcutaneal administration of carrageenan in the Swiss albino mice using the murine air pouch model. Exudation; leukocytes; myeloperoxidase (MPO); adenosine deaminase (ADA); nitric oxide metabolites (NOx); tumor necrosis factor (TNF); interferon gamma (IFN-γ); interleukins (IL) IL-6, IL-17, IL-10, IL-4, and IL-2; nuclear transcription factor kappa B (NF-κB) activation and apoptosis were evaluated 24 h after the induction of inflammation. Treatment with etanercept significantly inhibited exudate concentrations; leukocyte count; MPO and ADA activities; NOx, TNF, IFN-γ, and IL-17 levels; and NF-kappa B activation (
p
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| doi_str_mv | 10.1007/s00210-015-1162-x |
| format | Article |
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p
< 0.05). Etanercept induced apoptosis, reducing the number of viable neutrophils without increasing necrosis (
p
< 0.05). Our results suggest that the anti-inflammatory mechanism of action of etanercept may be via decrease of NF-κB activation. This effect promoted the reduction of pro-inflammatory cytokines and NOx and the induction of neutrophil apoptosis. The effect of etanercept upon neutrophils apoptosis may indicate the use of this drug therapy in the early stage of rheumatoid arthritis disease.</description><identifier>ISSN: 0028-1298</identifier><identifier>EISSN: 1432-1912</identifier><identifier>DOI: 10.1007/s00210-015-1162-x</identifier><identifier>PMID: 26255064</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Apoptosis - drug effects ; Apoptosis - physiology ; Arthritis, Rheumatoid - chemically induced ; Arthritis, Rheumatoid - drug therapy ; Arthritis, Rheumatoid - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Carrageenan - toxicity ; Disease Models, Animal ; Etanercept - pharmacology ; Etanercept - therapeutic use ; Immunosuppressive Agents - pharmacology ; Immunosuppressive Agents - therapeutic use ; Inflammation - chemically induced ; Inflammation - drug therapy ; Inflammation - metabolism ; Inflammation Mediators - antagonists & inhibitors ; Inflammation Mediators - metabolism ; Leukocytes - drug effects ; Leukocytes - metabolism ; Mice ; Neurosciences ; NF-kappa B - antagonists & inhibitors ; NF-kappa B - metabolism ; Original Article ; Pharmacology/Toxicology</subject><ispartof>Naunyn-Schmiedeberg's archives of pharmacology, 2015-12, Vol.388 (12), p.1247-1257</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-e2fdea4baab4cea83ec0272f88424d2a46d481f6506c2a8bfde9cf8e505b2f6d3</citedby><cites>FETCH-LOGICAL-c442t-e2fdea4baab4cea83ec0272f88424d2a46d481f6506c2a8bfde9cf8e505b2f6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00210-015-1162-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00210-015-1162-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26255064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mattei, Rodrigo Antônio</creatorcontrib><creatorcontrib>Dalmarco, Eduardo Monguilhott</creatorcontrib><creatorcontrib>Fröde, Tânia Silvia</creatorcontrib><title>Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model</title><title>Naunyn-Schmiedeberg's archives of pharmacology</title><addtitle>Naunyn-Schmiedeberg's Arch Pharmacol</addtitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><description>Rheumatoid arthritis (RA) is one of several inflammatory and autoimmune diseases that affect approximately 1 % of world’s population. The development of TNF inhibitors in the last decade represents a great advance in the treatment of mild and severe forms of RA. Etanercept is one of these drugs that is useful for RA treatment, but the mechanism of inhibition of the signaling pathway of inflammation was not completely elucidated. This study was conducted to evaluate the anti-inflammatory effect of etanercept in comparison to reference drugs (dexamethasone and indomethacin). Inflammation was induced by subcutaneal administration of carrageenan in the Swiss albino mice using the murine air pouch model. Exudation; leukocytes; myeloperoxidase (MPO); adenosine deaminase (ADA); nitric oxide metabolites (NOx); tumor necrosis factor (TNF); interferon gamma (IFN-γ); interleukins (IL) IL-6, IL-17, IL-10, IL-4, and IL-2; nuclear transcription factor kappa B (NF-κB) activation and apoptosis were evaluated 24 h after the induction of inflammation. Treatment with etanercept significantly inhibited exudate concentrations; leukocyte count; MPO and ADA activities; NOx, TNF, IFN-γ, and IL-17 levels; and NF-kappa B activation (
p
< 0.05). Etanercept induced apoptosis, reducing the number of viable neutrophils without increasing necrosis (
p
< 0.05). Our results suggest that the anti-inflammatory mechanism of action of etanercept may be via decrease of NF-κB activation. This effect promoted the reduction of pro-inflammatory cytokines and NOx and the induction of neutrophil apoptosis. The effect of etanercept upon neutrophils apoptosis may indicate the use of this drug therapy in the early stage of rheumatoid arthritis disease.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Arthritis, Rheumatoid - chemically induced</subject><subject>Arthritis, Rheumatoid - drug therapy</subject><subject>Arthritis, Rheumatoid - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carrageenan - toxicity</subject><subject>Disease Models, Animal</subject><subject>Etanercept - pharmacology</subject><subject>Etanercept - therapeutic use</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Immunosuppressive Agents - therapeutic use</subject><subject>Inflammation - chemically induced</subject><subject>Inflammation - drug therapy</subject><subject>Inflammation - metabolism</subject><subject>Inflammation Mediators - antagonists & inhibitors</subject><subject>Inflammation Mediators - metabolism</subject><subject>Leukocytes - drug effects</subject><subject>Leukocytes - metabolism</subject><subject>Mice</subject><subject>Neurosciences</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NF-kappa B - metabolism</subject><subject>Original Article</subject><subject>Pharmacology/Toxicology</subject><issn>0028-1298</issn><issn>1432-1912</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kUFr3DAQhUVpaTZpf0AvRdBLLm6lWdmWjyUkTSDQS3sWY2mcONiSI8kh---j7aalBHIamPnemxkeY5-k-CqFaL8lIUCKSsi6krKB6vEN20i1hUp2Et6yTRnrSkKnj9hxSndCiEbW9Xt2BA3UtWjUht2fZ_QULS2Zo5tHP6YcMY_B8yXSA_mceL4lPvphwnnGHOKOR0pL8Gnfdaslx_sdtxgj3hB59KX9RzOvcfTEcYx8Cau95XNwNH1g7wacEn18rifs98X5r7PL6vrnj6uz79eVVQpyRTA4QtUj9soS6i1ZAS0MWitQDlA1Tmk5NOUNC6j7Qnd20FSLuoehcdsTdnrwXWK4XyllM4_J0jSVf8OajGy3rWo7IXRBv7xA78IafbluTzVad1CLQskDZWNIKdJgljjOGHdGCrPPwxzyMCUPs8_DPBbN52fntZ_J_VP8DaAAcABSGfkbiv-tftX1CeSgmLU</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Mattei, Rodrigo Antônio</creator><creator>Dalmarco, Eduardo Monguilhott</creator><creator>Fröde, Tânia Silvia</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20151201</creationdate><title>Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model</title><author>Mattei, Rodrigo Antônio ; Dalmarco, Eduardo Monguilhott ; Fröde, Tânia Silvia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-e2fdea4baab4cea83ec0272f88424d2a46d481f6506c2a8bfde9cf8e505b2f6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - physiology</topic><topic>Arthritis, Rheumatoid - chemically induced</topic><topic>Arthritis, Rheumatoid - drug therapy</topic><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carrageenan - toxicity</topic><topic>Disease Models, Animal</topic><topic>Etanercept - pharmacology</topic><topic>Etanercept - therapeutic use</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Immunosuppressive Agents - therapeutic use</topic><topic>Inflammation - chemically induced</topic><topic>Inflammation - drug therapy</topic><topic>Inflammation - metabolism</topic><topic>Inflammation Mediators - antagonists & inhibitors</topic><topic>Inflammation Mediators - metabolism</topic><topic>Leukocytes - drug effects</topic><topic>Leukocytes - metabolism</topic><topic>Mice</topic><topic>Neurosciences</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NF-kappa B - metabolism</topic><topic>Original Article</topic><topic>Pharmacology/Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mattei, Rodrigo Antônio</creatorcontrib><creatorcontrib>Dalmarco, Eduardo Monguilhott</creatorcontrib><creatorcontrib>Fröde, Tânia Silvia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mattei, Rodrigo Antônio</au><au>Dalmarco, Eduardo Monguilhott</au><au>Fröde, Tânia Silvia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model</atitle><jtitle>Naunyn-Schmiedeberg's archives of pharmacology</jtitle><stitle>Naunyn-Schmiedeberg's Arch Pharmacol</stitle><addtitle>Naunyn Schmiedebergs Arch Pharmacol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>388</volume><issue>12</issue><spage>1247</spage><epage>1257</epage><pages>1247-1257</pages><issn>0028-1298</issn><eissn>1432-1912</eissn><abstract>Rheumatoid arthritis (RA) is one of several inflammatory and autoimmune diseases that affect approximately 1 % of world’s population. The development of TNF inhibitors in the last decade represents a great advance in the treatment of mild and severe forms of RA. Etanercept is one of these drugs that is useful for RA treatment, but the mechanism of inhibition of the signaling pathway of inflammation was not completely elucidated. This study was conducted to evaluate the anti-inflammatory effect of etanercept in comparison to reference drugs (dexamethasone and indomethacin). Inflammation was induced by subcutaneal administration of carrageenan in the Swiss albino mice using the murine air pouch model. Exudation; leukocytes; myeloperoxidase (MPO); adenosine deaminase (ADA); nitric oxide metabolites (NOx); tumor necrosis factor (TNF); interferon gamma (IFN-γ); interleukins (IL) IL-6, IL-17, IL-10, IL-4, and IL-2; nuclear transcription factor kappa B (NF-κB) activation and apoptosis were evaluated 24 h after the induction of inflammation. Treatment with etanercept significantly inhibited exudate concentrations; leukocyte count; MPO and ADA activities; NOx, TNF, IFN-γ, and IL-17 levels; and NF-kappa B activation (
p
< 0.05). Etanercept induced apoptosis, reducing the number of viable neutrophils without increasing necrosis (
p
< 0.05). Our results suggest that the anti-inflammatory mechanism of action of etanercept may be via decrease of NF-κB activation. This effect promoted the reduction of pro-inflammatory cytokines and NOx and the induction of neutrophil apoptosis. The effect of etanercept upon neutrophils apoptosis may indicate the use of this drug therapy in the early stage of rheumatoid arthritis disease.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26255064</pmid><doi>10.1007/s00210-015-1162-x</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects Apoptosis - physiology Arthritis, Rheumatoid - chemically induced Arthritis, Rheumatoid - drug therapy Arthritis, Rheumatoid - metabolism Biomedical and Life Sciences Biomedicine Carrageenan - toxicity Disease Models, Animal Etanercept - pharmacology Etanercept - therapeutic use Immunosuppressive Agents - pharmacology Immunosuppressive Agents - therapeutic use Inflammation - chemically induced Inflammation - drug therapy Inflammation - metabolism Inflammation Mediators - antagonists & inhibitors Inflammation Mediators - metabolism Leukocytes - drug effects Leukocytes - metabolism Mice Neurosciences NF-kappa B - antagonists & inhibitors NF-kappa B - metabolism Original Article Pharmacology/Toxicology |
| title | Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model |
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