Characterization of the gltF gene product of Escherichia coli
The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits ( gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site f...
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| Veröffentlicht in: | FEMS microbiology letters 1999-10, Vol.179 (1), p.79-84 |
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| container_title | FEMS microbiology letters |
| container_volume | 179 |
| creator | Grassl, Guntram Bufe, Bernd Müller, Birgit Rösel, Marc Kleiner, Diethelm |
| description | The
glt operon of
Escherichia coli comprises the structural genes for the glutamate synthase subunits (
gltB and
gltD) and
gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between
gltD and
gltF contains a weak promoter and a translation initiation site for
gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A
gltF::Km
R insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism. |
| doi_str_mv | 10.1016/S0378-1097(99)00397-3 |
| format | Article |
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glt operon of
Escherichia coli comprises the structural genes for the glutamate synthase subunits (
gltB and
gltD) and
gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between
gltD and
gltF contains a weak promoter and a translation initiation site for
gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A
gltF::Km
R insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(99)00397-3</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Escherichia coli ; gltF ; gltF gene ; GltF protein ; Glutamate synthase ; Nitrogen regulation ; Periplasmic protein</subject><ispartof>FEMS microbiology letters, 1999-10, Vol.179 (1), p.79-84</ispartof><rights>1999 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Grassl, Guntram</creatorcontrib><creatorcontrib>Bufe, Bernd</creatorcontrib><creatorcontrib>Müller, Birgit</creatorcontrib><creatorcontrib>Rösel, Marc</creatorcontrib><creatorcontrib>Kleiner, Diethelm</creatorcontrib><title>Characterization of the gltF gene product of Escherichia coli</title><title>FEMS microbiology letters</title><description>The
glt operon of
Escherichia coli comprises the structural genes for the glutamate synthase subunits (
gltB and
gltD) and
gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between
gltD and
gltF contains a weak promoter and a translation initiation site for
gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A
gltF::Km
R insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism.</description><subject>Escherichia coli</subject><subject>gltF</subject><subject>gltF gene</subject><subject>GltF protein</subject><subject>Glutamate synthase</subject><subject>Nitrogen regulation</subject><subject>Periplasmic protein</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNo9kE1Lw0AURQdRsFZ_gpCV6CL6Ji-Zj4WIhFaFggt1PUwmL81ITDQzdeGvN23F1V3cw-VyGDvncM2Bi5sXQKlSDlpean0FgFqmeMBmvJB5KrRQh2z2jxyzkxDeASDPQMzYbdna0bpIo_-x0Q99MjRJbClZd3GZrKmn5HMc6o2L22IRXDuRrvU2cUPnT9lRY7tAZ385Z2_LxWv5mK6eH57K-1VKmZIxRaxUYxsliUSOdYUcM-IqLxoLBehKcUGkJBS5ACWd0rbRykkt0DlbyQbn7GK_O3352lCI5sMHR11nexo2wXCJElUmJvBuD9L05tvTaILz1Duq_UgumnrwhoPZajM7bWbrxGhtdtoM4i8jSGBz</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Grassl, Guntram</creator><creator>Bufe, Bernd</creator><creator>Müller, Birgit</creator><creator>Rösel, Marc</creator><creator>Kleiner, Diethelm</creator><general>Elsevier B.V</general><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>19991001</creationdate><title>Characterization of the gltF gene product of Escherichia coli</title><author>Grassl, Guntram ; Bufe, Bernd ; Müller, Birgit ; Rösel, Marc ; Kleiner, Diethelm</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e287t-33b8faf87ee643db3132e1845fa0509b816ee870546087c89af98c7963ccab7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Escherichia coli</topic><topic>gltF</topic><topic>gltF gene</topic><topic>GltF protein</topic><topic>Glutamate synthase</topic><topic>Nitrogen regulation</topic><topic>Periplasmic protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grassl, Guntram</creatorcontrib><creatorcontrib>Bufe, Bernd</creatorcontrib><creatorcontrib>Müller, Birgit</creatorcontrib><creatorcontrib>Rösel, Marc</creatorcontrib><creatorcontrib>Kleiner, Diethelm</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grassl, Guntram</au><au>Bufe, Bernd</au><au>Müller, Birgit</au><au>Rösel, Marc</au><au>Kleiner, Diethelm</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the gltF gene product of Escherichia coli</atitle><jtitle>FEMS microbiology letters</jtitle><date>1999-10-01</date><risdate>1999</risdate><volume>179</volume><issue>1</issue><spage>79</spage><epage>84</epage><pages>79-84</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>The
glt operon of
Escherichia coli comprises the structural genes for the glutamate synthase subunits (
gltB and
gltD) and
gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between
gltD and
gltF contains a weak promoter and a translation initiation site for
gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A
gltF::Km
R insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S0378-1097(99)00397-3</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-10, Vol.179 (1), p.79-84 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17373826 |
| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Escherichia coli gltF gltF gene GltF protein Glutamate synthase Nitrogen regulation Periplasmic protein |
| title | Characterization of the gltF gene product of Escherichia coli |
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