Postsynthetic Domain Assembly with NpuDnaE and SspDnaB Split Inteins

Inteins are protein segments embedded in frame within a precursor sequence that catalyze a self-excision reaction and ligate the flanking sequences with a standard peptide bond. Split inteins are expressed as two separate polypeptide fragments and trans-splice upon subunit association. Split inteins...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2015-11, Vol.177 (5), p.1137-1151
Hauptverfasser:	Demonte, Daniel, Li, Naiyi, Park, Sheldon
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biotechnology Chemistry Chemistry and Materials Science DNA Polymerase III - genetics DNA Polymerase III - metabolism DnaB Helicases - genetics DnaB Helicases - metabolism E coli Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Inteins - physiology Peptides polypeptides protein solubility Protein Splicing - physiology Protein Structure, Tertiary protein synthesis Proteins Solubility
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