Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall
Reconstruction of the bladder wall via in vitro differentiated stem cells on an appropriate scaffold could be used in such conditions as cancer and neurogenic urinary bladder. This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (...
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| Veröffentlicht in: | Journal of tissue engineering and regenerative medicine 2015-11, Vol.9 (11), p.1268-1276 |
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| creator | Shoae-Hassani, Alireza Mortazavi-Tabatabaei, Seyed Abdolreza Sharif, Shiva Seifalian, Alexander Marcus Azimi, Alireza Samadikuchaksaraei, Ali Verdi, Javad |
| description | Reconstruction of the bladder wall via in vitro differentiated stem cells on an appropriate scaffold could be used in such conditions as cancer and neurogenic urinary bladder. This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (urothelium) on nanofibrous silk–collagen scaffolds, for construction of the urinary bladder wall. After passage 4, EnSCs were induced by keratinocyte growth factor (KGF) and epidermal growth factor (EGF) and seeded on electrospun collagen‐V, silk and silk–collagen nanofibres. Later we tested urothelium‐specific genes and proteins (uroplakin‐Ia, uroplakin‐Ib, uroplakin‐II, uroplakin‐III and cytokeratin 20) by immunocytochemistry, RT–PCR and western blot analyses. Scanning electron microscopy (SEM) and histology were used to detect cell–matrix interactions. DMEM/F12 supplemented by KGF and EGF induced EnSCs to express urothelial cell‐specific genes and proteins. Either collagen, silk or silk–collagen scaffolds promoted cell proliferation. The nanofibrous silk–collagen scaffolds provided a three‐dimensional (3D) structure to maximize cell‐matrix penetration and increase differentiation of the EnSCs. Human EnSCs seeded on 3D nanofibrous silk–collagen scaffolds and differentiated to urothelial cells provide a suitable source for potential use in bladder wall reconstruction in women. Copyright © 2013 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/term.1632 |
| format | Article |
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This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (urothelium) on nanofibrous silk–collagen scaffolds, for construction of the urinary bladder wall. After passage 4, EnSCs were induced by keratinocyte growth factor (KGF) and epidermal growth factor (EGF) and seeded on electrospun collagen‐V, silk and silk–collagen nanofibres. Later we tested urothelium‐specific genes and proteins (uroplakin‐Ia, uroplakin‐Ib, uroplakin‐II, uroplakin‐III and cytokeratin 20) by immunocytochemistry, RT–PCR and western blot analyses. Scanning electron microscopy (SEM) and histology were used to detect cell–matrix interactions. DMEM/F12 supplemented by KGF and EGF induced EnSCs to express urothelial cell‐specific genes and proteins. Either collagen, silk or silk–collagen scaffolds promoted cell proliferation. The nanofibrous silk–collagen scaffolds provided a three‐dimensional (3D) structure to maximize cell‐matrix penetration and increase differentiation of the EnSCs. Human EnSCs seeded on 3D nanofibrous silk–collagen scaffolds and differentiated to urothelial cells provide a suitable source for potential use in bladder wall reconstruction in women. Copyright © 2013 John Wiley & Sons, Ltd.</description><identifier>ISSN: 1932-6254</identifier><identifier>EISSN: 1932-7005</identifier><identifier>DOI: 10.1002/term.1632</identifier><identifier>PMID: 23319462</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adult ; Biocompatible Materials - pharmacology ; biomarker ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Collagen - chemistry ; cytokeratin ; differentiation ; Endometrium - cytology ; Enzyme-Linked Immunosorbent Assay ; Epidermal Growth Factor - metabolism ; Female ; Fibroblast Growth Factor 7 - metabolism ; Humans ; Immunohistochemistry ; Keratinocytes - cytology ; Microscopy, Electron, Scanning ; Nanofibers - chemistry ; Phenotype ; Regenerative medicine ; Silk - chemistry ; stem cell ; Stem Cells - cytology ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Urinary Bladder - pathology ; uroplakin ; urothelium ; Urothelium - cytology ; Urothelium - pathology</subject><ispartof>Journal of tissue engineering and regenerative medicine, 2015-11, Vol.9 (11), p.1268-1276</ispartof><rights>Copyright © 2013 John Wiley & Sons, Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4602-178bab96beaf8f37a5effcf569850f85773b5dcb720a6ca322c4c09d5f6edcf83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fterm.1632$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fterm.1632$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23319462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shoae-Hassani, Alireza</creatorcontrib><creatorcontrib>Mortazavi-Tabatabaei, Seyed Abdolreza</creatorcontrib><creatorcontrib>Sharif, Shiva</creatorcontrib><creatorcontrib>Seifalian, Alexander Marcus</creatorcontrib><creatorcontrib>Azimi, Alireza</creatorcontrib><creatorcontrib>Samadikuchaksaraei, Ali</creatorcontrib><creatorcontrib>Verdi, Javad</creatorcontrib><title>Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall</title><title>Journal of tissue engineering and regenerative medicine</title><addtitle>J Tissue Eng Regen Med</addtitle><description>Reconstruction of the bladder wall via in vitro differentiated stem cells on an appropriate scaffold could be used in such conditions as cancer and neurogenic urinary bladder. This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (urothelium) on nanofibrous silk–collagen scaffolds, for construction of the urinary bladder wall. After passage 4, EnSCs were induced by keratinocyte growth factor (KGF) and epidermal growth factor (EGF) and seeded on electrospun collagen‐V, silk and silk–collagen nanofibres. Later we tested urothelium‐specific genes and proteins (uroplakin‐Ia, uroplakin‐Ib, uroplakin‐II, uroplakin‐III and cytokeratin 20) by immunocytochemistry, RT–PCR and western blot analyses. Scanning electron microscopy (SEM) and histology were used to detect cell–matrix interactions. DMEM/F12 supplemented by KGF and EGF induced EnSCs to express urothelial cell‐specific genes and proteins. Either collagen, silk or silk–collagen scaffolds promoted cell proliferation. The nanofibrous silk–collagen scaffolds provided a three‐dimensional (3D) structure to maximize cell‐matrix penetration and increase differentiation of the EnSCs. Human EnSCs seeded on 3D nanofibrous silk–collagen scaffolds and differentiated to urothelial cells provide a suitable source for potential use in bladder wall reconstruction in women. Copyright © 2013 John Wiley & Sons, Ltd.</description><subject>Adult</subject><subject>Biocompatible Materials - pharmacology</subject><subject>biomarker</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Collagen - chemistry</subject><subject>cytokeratin</subject><subject>differentiation</subject><subject>Endometrium - cytology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Female</subject><subject>Fibroblast Growth Factor 7 - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratinocytes - cytology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nanofibers - chemistry</subject><subject>Phenotype</subject><subject>Regenerative medicine</subject><subject>Silk - chemistry</subject><subject>stem cell</subject><subject>Stem Cells - cytology</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Urinary Bladder - pathology</subject><subject>uroplakin</subject><subject>urothelium</subject><subject>Urothelium - cytology</subject><subject>Urothelium - pathology</subject><issn>1932-6254</issn><issn>1932-7005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkFvFSEQxzdGY2v14BcwJF68bMvCArvezGv71FRN9Bm9EZYd-mhZqMCm9jP6pWTzXnvw5Ilh5vefGYapqpcNPm4wJicZ4nTccEoeVYdNT0ktMGaP9zYnrD2onqV0VZyMM_q0OiCUNn3LyWH159QaAxF8tirb4FEwaDtPyiPwY5ggR6scShkmpMG5hKzPAc0x5C24JbTzFqFCeRsB6tFO4FNJVYJe-WDsEMOcULLuutbBOXUJHiWtjAlufItKKTXn4MLlQi3pUIQU5qgBmRDLRQefcpz1fX-ldOnAehXv0ODUOEJEt8q559UTo1yCF_vzqPp-frZZva8vvqw_rN5d1LrlmNSN6AY19HwAZTpDhWJgjDaM9x3DpmNC0IGNehAEK64VJUS3GvcjMxxGbTp6VL3Z5b2J4dcMKcvJpqVx5aG8QTaCsp6UMvh_0MI1oqUFff0PelWGUKa4UKQXtO14U6hXe2oeJhjlTbRTmYO8_9ECnOyAW-vg7iHeYLmsilxWRS6rIjdnXz8tRlHUO4Ut3_z7QaHiteSCCiZ_fF7L09Xm58fz1Te5pn8BtLLGNw</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Shoae-Hassani, Alireza</creator><creator>Mortazavi-Tabatabaei, Seyed Abdolreza</creator><creator>Sharif, Shiva</creator><creator>Seifalian, Alexander Marcus</creator><creator>Azimi, Alireza</creator><creator>Samadikuchaksaraei, Ali</creator><creator>Verdi, Javad</creator><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>201511</creationdate><title>Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall</title><author>Shoae-Hassani, Alireza ; Mortazavi-Tabatabaei, Seyed Abdolreza ; Sharif, Shiva ; Seifalian, Alexander Marcus ; Azimi, Alireza ; Samadikuchaksaraei, Ali ; Verdi, Javad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4602-178bab96beaf8f37a5effcf569850f85773b5dcb720a6ca322c4c09d5f6edcf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Biocompatible Materials - pharmacology</topic><topic>biomarker</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Collagen - chemistry</topic><topic>cytokeratin</topic><topic>differentiation</topic><topic>Endometrium - cytology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Female</topic><topic>Fibroblast Growth Factor 7 - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keratinocytes - cytology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nanofibers - chemistry</topic><topic>Phenotype</topic><topic>Regenerative medicine</topic><topic>Silk - chemistry</topic><topic>stem cell</topic><topic>Stem Cells - cytology</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Urinary Bladder - pathology</topic><topic>uroplakin</topic><topic>urothelium</topic><topic>Urothelium - cytology</topic><topic>Urothelium - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shoae-Hassani, Alireza</creatorcontrib><creatorcontrib>Mortazavi-Tabatabaei, Seyed Abdolreza</creatorcontrib><creatorcontrib>Sharif, Shiva</creatorcontrib><creatorcontrib>Seifalian, Alexander Marcus</creatorcontrib><creatorcontrib>Azimi, Alireza</creatorcontrib><creatorcontrib>Samadikuchaksaraei, Ali</creatorcontrib><creatorcontrib>Verdi, Javad</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Journal of tissue engineering and regenerative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shoae-Hassani, Alireza</au><au>Mortazavi-Tabatabaei, Seyed Abdolreza</au><au>Sharif, Shiva</au><au>Seifalian, Alexander Marcus</au><au>Azimi, Alireza</au><au>Samadikuchaksaraei, Ali</au><au>Verdi, Javad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall</atitle><jtitle>Journal of tissue engineering and regenerative medicine</jtitle><addtitle>J Tissue Eng Regen Med</addtitle><date>2015-11</date><risdate>2015</risdate><volume>9</volume><issue>11</issue><spage>1268</spage><epage>1276</epage><pages>1268-1276</pages><issn>1932-6254</issn><eissn>1932-7005</eissn><abstract>Reconstruction of the bladder wall via in vitro differentiated stem cells on an appropriate scaffold could be used in such conditions as cancer and neurogenic urinary bladder. This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (urothelium) on nanofibrous silk–collagen scaffolds, for construction of the urinary bladder wall. After passage 4, EnSCs were induced by keratinocyte growth factor (KGF) and epidermal growth factor (EGF) and seeded on electrospun collagen‐V, silk and silk–collagen nanofibres. Later we tested urothelium‐specific genes and proteins (uroplakin‐Ia, uroplakin‐Ib, uroplakin‐II, uroplakin‐III and cytokeratin 20) by immunocytochemistry, RT–PCR and western blot analyses. Scanning electron microscopy (SEM) and histology were used to detect cell–matrix interactions. DMEM/F12 supplemented by KGF and EGF induced EnSCs to express urothelial cell‐specific genes and proteins. Either collagen, silk or silk–collagen scaffolds promoted cell proliferation. The nanofibrous silk–collagen scaffolds provided a three‐dimensional (3D) structure to maximize cell‐matrix penetration and increase differentiation of the EnSCs. Human EnSCs seeded on 3D nanofibrous silk–collagen scaffolds and differentiated to urothelial cells provide a suitable source for potential use in bladder wall reconstruction in women. Copyright © 2013 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23319462</pmid><doi>10.1002/term.1632</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Biocompatible Materials - pharmacology biomarker Cell Culture Techniques Cell Differentiation Cells, Cultured Collagen - chemistry cytokeratin differentiation Endometrium - cytology Enzyme-Linked Immunosorbent Assay Epidermal Growth Factor - metabolism Female Fibroblast Growth Factor 7 - metabolism Humans Immunohistochemistry Keratinocytes - cytology Microscopy, Electron, Scanning Nanofibers - chemistry Phenotype Regenerative medicine Silk - chemistry stem cell Stem Cells - cytology Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry Urinary Bladder - pathology uroplakin urothelium Urothelium - cytology Urothelium - pathology |
| title | Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall |
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