Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?
Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of th...
Gespeichert in:
| Veröffentlicht in: | Viral immunology 2015-11, Vol.28 (9), p.54-508 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 508 |
|---|---|
| container_issue | 9 |
| container_start_page | 54 |
| container_title | Viral immunology |
| container_volume | 28 |
| creator | Ebrahim, Maryam Mirzaei, Vahid Bidaki, Reza Shabani, Ziba Daneshvar, Hamid Karimi-Googheri, Masoud Khaleghinia, Mehdi Afrooz, Mohammad Reza Yousefpoor, Yaser Kazemi Arababadi, Mohammad |
| description | Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of the present study was to evaluate the mRNA levels of MDA5 and RIG-1 in the peripheral blood immune cells of CHB patients in comparison to healthy controls. In this cross-sectional study, the mRNA levels of MDA5 and RIG-1 were examined in 60 CHB patients and 60 healthy controls using the real-time polymerase chain reaction (PCR) technique. Our results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in CHB patients when compared to healthy controls. Our results also revealed that mRNA levels of MDA5 and RIG-1 were not altered among CHB patients with various states of e-antigen of hepatitis B and HBV-DNA viral loads. According to the results presented here, it may be concluded that downregulation of MDA5 may be a responsible mechanism from several reasons, which leads to HBV persistence in CHB patients. |
| doi_str_mv | 10.1089/vim.2015.0056 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1735921630</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1731781642</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-28913138e063a25234ed3e34060189f8d5c825930b0c0f3a865f605905fc04943</originalsourceid><addsrcrecordid>eNqN0UtLw0AUBeBBFFurS7cy4MZN6p1nZlYSa20LiiDqNqSTCU3Jo84kPv69Ca0u3OhsLgwfB-49CJ0SGBNQ-vItL8cUiBgDCLmHhkSIMFA6lPtoCErRQFEuBujI-zUAKKnYIRpQyZVgXA7RNHIWPy5mAcFJleL7m0jg6cfGWe_zuvI48r42edLYFL_nzQpPVq6ucoPn1y94UWXWNB27OkYHWVJ4e7KbI_R8O32azIO7h9liEt0FhmnVBFRpwghTFiRLqKCM25RZxkECUTpTqTCKCs1gCQYyligpMglCg8gMcM3ZCF1sczeufm2tb-Iy98YWRVLZuvUxCZnQlEgG_6EkVERy2tHzX3Rdt67qFukU1ZxS1r0RCrbKuNp7Z7N44_IycZ8xgbivIu6qiPsq4r6Kzp_tUttladMf_X37DrAt6L-Tqipyu7Su-SP2C03_j_I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1729422333</pqid></control><display><type>article</type><title>Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Ebrahim, Maryam ; Mirzaei, Vahid ; Bidaki, Reza ; Shabani, Ziba ; Daneshvar, Hamid ; Karimi-Googheri, Masoud ; Khaleghinia, Mehdi ; Afrooz, Mohammad Reza ; Yousefpoor, Yaser ; Kazemi Arababadi, Mohammad</creator><creatorcontrib>Ebrahim, Maryam ; Mirzaei, Vahid ; Bidaki, Reza ; Shabani, Ziba ; Daneshvar, Hamid ; Karimi-Googheri, Masoud ; Khaleghinia, Mehdi ; Afrooz, Mohammad Reza ; Yousefpoor, Yaser ; Kazemi Arababadi, Mohammad</creatorcontrib><description>Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of the present study was to evaluate the mRNA levels of MDA5 and RIG-1 in the peripheral blood immune cells of CHB patients in comparison to healthy controls. In this cross-sectional study, the mRNA levels of MDA5 and RIG-1 were examined in 60 CHB patients and 60 healthy controls using the real-time polymerase chain reaction (PCR) technique. Our results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in CHB patients when compared to healthy controls. Our results also revealed that mRNA levels of MDA5 and RIG-1 were not altered among CHB patients with various states of e-antigen of hepatitis B and HBV-DNA viral loads. According to the results presented here, it may be concluded that downregulation of MDA5 may be a responsible mechanism from several reasons, which leads to HBV persistence in CHB patients.</description><identifier>ISSN: 0882-8245</identifier><identifier>EISSN: 1557-8976</identifier><identifier>DOI: 10.1089/vim.2015.0056</identifier><identifier>PMID: 26485346</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Cross-Sectional Studies ; DEAD Box Protein 58 ; DEAD-box RNA Helicases - biosynthesis ; DEAD-box RNA Helicases - genetics ; Female ; Gene Expression ; Gene Expression Profiling ; Hepatitis B e Antigens - blood ; Hepatitis B virus ; Hepatitis B virus - isolation & purification ; Hepatitis B, Chronic - pathology ; Humans ; Interferon-Induced Helicase, IFIH1 ; Male ; Original Research Articles ; Real-Time Polymerase Chain Reaction ; Receptors, Immunologic ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Viral Load</subject><ispartof>Viral immunology, 2015-11, Vol.28 (9), p.54-508</ispartof><rights>2015, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2015, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-28913138e063a25234ed3e34060189f8d5c825930b0c0f3a865f605905fc04943</citedby><cites>FETCH-LOGICAL-c398t-28913138e063a25234ed3e34060189f8d5c825930b0c0f3a865f605905fc04943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26485346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebrahim, Maryam</creatorcontrib><creatorcontrib>Mirzaei, Vahid</creatorcontrib><creatorcontrib>Bidaki, Reza</creatorcontrib><creatorcontrib>Shabani, Ziba</creatorcontrib><creatorcontrib>Daneshvar, Hamid</creatorcontrib><creatorcontrib>Karimi-Googheri, Masoud</creatorcontrib><creatorcontrib>Khaleghinia, Mehdi</creatorcontrib><creatorcontrib>Afrooz, Mohammad Reza</creatorcontrib><creatorcontrib>Yousefpoor, Yaser</creatorcontrib><creatorcontrib>Kazemi Arababadi, Mohammad</creatorcontrib><title>Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?</title><title>Viral immunology</title><addtitle>Viral Immunol</addtitle><description>Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of the present study was to evaluate the mRNA levels of MDA5 and RIG-1 in the peripheral blood immune cells of CHB patients in comparison to healthy controls. In this cross-sectional study, the mRNA levels of MDA5 and RIG-1 were examined in 60 CHB patients and 60 healthy controls using the real-time polymerase chain reaction (PCR) technique. Our results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in CHB patients when compared to healthy controls. Our results also revealed that mRNA levels of MDA5 and RIG-1 were not altered among CHB patients with various states of e-antigen of hepatitis B and HBV-DNA viral loads. According to the results presented here, it may be concluded that downregulation of MDA5 may be a responsible mechanism from several reasons, which leads to HBV persistence in CHB patients.</description><subject>Cross-Sectional Studies</subject><subject>DEAD Box Protein 58</subject><subject>DEAD-box RNA Helicases - biosynthesis</subject><subject>DEAD-box RNA Helicases - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Hepatitis B e Antigens - blood</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Hepatitis B, Chronic - pathology</subject><subject>Humans</subject><subject>Interferon-Induced Helicase, IFIH1</subject><subject>Male</subject><subject>Original Research Articles</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Immunologic</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Viral Load</subject><issn>0882-8245</issn><issn>1557-8976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqN0UtLw0AUBeBBFFurS7cy4MZN6p1nZlYSa20LiiDqNqSTCU3Jo84kPv69Ca0u3OhsLgwfB-49CJ0SGBNQ-vItL8cUiBgDCLmHhkSIMFA6lPtoCErRQFEuBujI-zUAKKnYIRpQyZVgXA7RNHIWPy5mAcFJleL7m0jg6cfGWe_zuvI48r42edLYFL_nzQpPVq6ucoPn1y94UWXWNB27OkYHWVJ4e7KbI_R8O32azIO7h9liEt0FhmnVBFRpwghTFiRLqKCM25RZxkECUTpTqTCKCs1gCQYyligpMglCg8gMcM3ZCF1sczeufm2tb-Iy98YWRVLZuvUxCZnQlEgG_6EkVERy2tHzX3Rdt67qFukU1ZxS1r0RCrbKuNp7Z7N44_IycZ8xgbivIu6qiPsq4r6Kzp_tUttladMf_X37DrAt6L-Tqipyu7Su-SP2C03_j_I</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Ebrahim, Maryam</creator><creator>Mirzaei, Vahid</creator><creator>Bidaki, Reza</creator><creator>Shabani, Ziba</creator><creator>Daneshvar, Hamid</creator><creator>Karimi-Googheri, Masoud</creator><creator>Khaleghinia, Mehdi</creator><creator>Afrooz, Mohammad Reza</creator><creator>Yousefpoor, Yaser</creator><creator>Kazemi Arababadi, Mohammad</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20151101</creationdate><title>Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?</title><author>Ebrahim, Maryam ; Mirzaei, Vahid ; Bidaki, Reza ; Shabani, Ziba ; Daneshvar, Hamid ; Karimi-Googheri, Masoud ; Khaleghinia, Mehdi ; Afrooz, Mohammad Reza ; Yousefpoor, Yaser ; Kazemi Arababadi, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-28913138e063a25234ed3e34060189f8d5c825930b0c0f3a865f605905fc04943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cross-Sectional Studies</topic><topic>DEAD Box Protein 58</topic><topic>DEAD-box RNA Helicases - biosynthesis</topic><topic>DEAD-box RNA Helicases - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Hepatitis B e Antigens - blood</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - isolation & purification</topic><topic>Hepatitis B, Chronic - pathology</topic><topic>Humans</topic><topic>Interferon-Induced Helicase, IFIH1</topic><topic>Male</topic><topic>Original Research Articles</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Immunologic</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Viral Load</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebrahim, Maryam</creatorcontrib><creatorcontrib>Mirzaei, Vahid</creatorcontrib><creatorcontrib>Bidaki, Reza</creatorcontrib><creatorcontrib>Shabani, Ziba</creatorcontrib><creatorcontrib>Daneshvar, Hamid</creatorcontrib><creatorcontrib>Karimi-Googheri, Masoud</creatorcontrib><creatorcontrib>Khaleghinia, Mehdi</creatorcontrib><creatorcontrib>Afrooz, Mohammad Reza</creatorcontrib><creatorcontrib>Yousefpoor, Yaser</creatorcontrib><creatorcontrib>Kazemi Arababadi, Mohammad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Viral immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebrahim, Maryam</au><au>Mirzaei, Vahid</au><au>Bidaki, Reza</au><au>Shabani, Ziba</au><au>Daneshvar, Hamid</au><au>Karimi-Googheri, Masoud</au><au>Khaleghinia, Mehdi</au><au>Afrooz, Mohammad Reza</au><au>Yousefpoor, Yaser</au><au>Kazemi Arababadi, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?</atitle><jtitle>Viral immunology</jtitle><addtitle>Viral Immunol</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>28</volume><issue>9</issue><spage>54</spage><epage>508</epage><pages>54-508</pages><issn>0882-8245</issn><eissn>1557-8976</eissn><abstract>Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of the present study was to evaluate the mRNA levels of MDA5 and RIG-1 in the peripheral blood immune cells of CHB patients in comparison to healthy controls. In this cross-sectional study, the mRNA levels of MDA5 and RIG-1 were examined in 60 CHB patients and 60 healthy controls using the real-time polymerase chain reaction (PCR) technique. Our results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in CHB patients when compared to healthy controls. Our results also revealed that mRNA levels of MDA5 and RIG-1 were not altered among CHB patients with various states of e-antigen of hepatitis B and HBV-DNA viral loads. According to the results presented here, it may be concluded that downregulation of MDA5 may be a responsible mechanism from several reasons, which leads to HBV persistence in CHB patients.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>26485346</pmid><doi>10.1089/vim.2015.0056</doi><tpages>455</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-8245 |
| ispartof | Viral immunology, 2015-11, Vol.28 (9), p.54-508 |
| issn | 0882-8245 1557-8976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1735921630 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Cross-Sectional Studies DEAD Box Protein 58 DEAD-box RNA Helicases - biosynthesis DEAD-box RNA Helicases - genetics Female Gene Expression Gene Expression Profiling Hepatitis B e Antigens - blood Hepatitis B virus Hepatitis B virus - isolation & purification Hepatitis B, Chronic - pathology Humans Interferon-Induced Helicase, IFIH1 Male Original Research Articles Real-Time Polymerase Chain Reaction Receptors, Immunologic RNA, Messenger - analysis RNA, Messenger - genetics Viral Load |
| title | Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection? |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T18%3A25%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Are%20RIG-1%20and%20MDA5%20Expressions%20Associated%20with%20Chronic%20HBV%20Infection?&rft.jtitle=Viral%20immunology&rft.au=Ebrahim,%20Maryam&rft.date=2015-11-01&rft.volume=28&rft.issue=9&rft.spage=54&rft.epage=508&rft.pages=54-508&rft.issn=0882-8245&rft.eissn=1557-8976&rft_id=info:doi/10.1089/vim.2015.0056&rft_dat=%3Cproquest_cross%3E1731781642%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1729422333&rft_id=info:pmid/26485346&rfr_iscdi=true |