Aquaporins in the honeybee crop—a new function for an old organ
Nectar used by bees as a food source is collected and stored in the crop, where it is transported and converted into honey. The production of honey involves water uptake from nectar. However, the crop is a portion of the insect foregut that has been characterized solely as a food storage organ. Aqua...
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| Veröffentlicht in: | Protoplasma 2014-11, Vol.251 (6), p.1441-1447 |
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| creator | Serrão, José Eduardo do Carmo Queiroz Fialho, Maria Azevedo, Dihego Oliveira Zanuncio, José Cola |
| description | Nectar used by bees as a food source is collected and stored in the crop, where it is transported and converted into honey. The production of honey involves water uptake from nectar. However, the crop is a portion of the insect foregut that has been characterized solely as a food storage organ. Aquaporins are integral membrane proteins that function as specific canal for water transport and are abundant in tissues with high water permeability. In this study, we detected five predicted genes for aquaporins in the gut of the honeybee
Apis mellifera
. We evaluated the aquaporins’ localization in the crop by using an anti-aquaporin antibody produced against the peptide sequence from one of the expressed genes, which was detected in the crop epithelium, particularly in the apical portions of the cells. Furthermore, we also showed an increase in sugar concentration in a sucrose solution collected from the crop lumen a few minutes after feeding, indicating that water uptake occurs during storage of nectar in the crop, suggesting a previously unidentified function for the honeybee crop. |
| doi_str_mv | 10.1007/s00709-014-0645-9 |
| format | Article |
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Apis mellifera
. We evaluated the aquaporins’ localization in the crop by using an anti-aquaporin antibody produced against the peptide sequence from one of the expressed genes, which was detected in the crop epithelium, particularly in the apical portions of the cells. Furthermore, we also showed an increase in sugar concentration in a sucrose solution collected from the crop lumen a few minutes after feeding, indicating that water uptake occurs during storage of nectar in the crop, suggesting a previously unidentified function for the honeybee crop.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-014-0645-9</identifier><identifier>PMID: 24756368</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Animals ; Apis mellifera ; Aquaporins - genetics ; Aquaporins - metabolism ; Bees - anatomy & histology ; Bees - genetics ; Bees - metabolism ; Biomedical and Life Sciences ; Cell Biology ; Digestive System - metabolism ; Epithelium - metabolism ; Genes, Insect ; Hierarchy, Social ; Honey ; Immunohistochemistry ; Life Sciences ; Organ Specificity ; Original Article ; Plant Sciences ; Polymerase Chain Reaction ; Sucrose - metabolism ; Zoology</subject><ispartof>Protoplasma, 2014-11, Vol.251 (6), p.1441-1447</ispartof><rights>Springer-Verlag Wien 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-25b83a37a038087cfbf94ee4e6d801249c664b167cd821a1a0f62e09f37a53193</citedby><cites>FETCH-LOGICAL-c481t-25b83a37a038087cfbf94ee4e6d801249c664b167cd821a1a0f62e09f37a53193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00709-014-0645-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00709-014-0645-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24756368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Serrão, José Eduardo</creatorcontrib><creatorcontrib>do Carmo Queiroz Fialho, Maria</creatorcontrib><creatorcontrib>Azevedo, Dihego Oliveira</creatorcontrib><creatorcontrib>Zanuncio, José Cola</creatorcontrib><title>Aquaporins in the honeybee crop—a new function for an old organ</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Nectar used by bees as a food source is collected and stored in the crop, where it is transported and converted into honey. The production of honey involves water uptake from nectar. However, the crop is a portion of the insect foregut that has been characterized solely as a food storage organ. Aquaporins are integral membrane proteins that function as specific canal for water transport and are abundant in tissues with high water permeability. In this study, we detected five predicted genes for aquaporins in the gut of the honeybee
Apis mellifera
. We evaluated the aquaporins’ localization in the crop by using an anti-aquaporin antibody produced against the peptide sequence from one of the expressed genes, which was detected in the crop epithelium, particularly in the apical portions of the cells. Furthermore, we also showed an increase in sugar concentration in a sucrose solution collected from the crop lumen a few minutes after feeding, indicating that water uptake occurs during storage of nectar in the crop, suggesting a previously unidentified function for the honeybee crop.</description><subject>Animals</subject><subject>Apis mellifera</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Bees - anatomy & histology</subject><subject>Bees - genetics</subject><subject>Bees - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Digestive System - metabolism</subject><subject>Epithelium - metabolism</subject><subject>Genes, Insect</subject><subject>Hierarchy, Social</subject><subject>Honey</subject><subject>Immunohistochemistry</subject><subject>Life Sciences</subject><subject>Organ Specificity</subject><subject>Original Article</subject><subject>Plant Sciences</subject><subject>Polymerase Chain Reaction</subject><subject>Sucrose - metabolism</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkctKAzEUhoMoWi8P4EYCbtyMnpPbZJZFvEHBjYK7kJlmdKRNatJBuvMhfEKfxJRWEUHcnLM43_nP5SfkEOEUAcqzlANUBaAoQAlZVBtkgAploRDYJhkAcF6g5g87ZDelZwCQDOQ22WGilIorPSDD4UtvZyF2PtHO0_mTo0_Bu0XtHG1imH28vVvq3Stte9_Mu-BpGyK1nobJmIb4aP0-2WrtJLmDdd4j95cXd-fXxej26uZ8OCoaoXFeMFlrbnlpgWvQZdPWbSWcE06NNSATVaOUqFGVzVgztGihVcxB1eYWybHie-RkpTuL4aV3aW6mXWrcZGK9C30yWHJZMWRl-T-qUKMUXKmMHv9Cn0MffT5kSSmOeV2ZKVxR-SUpRdeaWeymNi4MgllaYVZWmGyFWVphlvserZX7eurG3x1fv88AWwEpl_yjiz9G_6n6CWntkbs</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Serrão, José Eduardo</creator><creator>do Carmo Queiroz Fialho, Maria</creator><creator>Azevedo, Dihego Oliveira</creator><creator>Zanuncio, José Cola</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>7SS</scope></search><sort><creationdate>20141101</creationdate><title>Aquaporins in the honeybee crop—a new function for an old organ</title><author>Serrão, José Eduardo ; 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The production of honey involves water uptake from nectar. However, the crop is a portion of the insect foregut that has been characterized solely as a food storage organ. Aquaporins are integral membrane proteins that function as specific canal for water transport and are abundant in tissues with high water permeability. In this study, we detected five predicted genes for aquaporins in the gut of the honeybee
Apis mellifera
. We evaluated the aquaporins’ localization in the crop by using an anti-aquaporin antibody produced against the peptide sequence from one of the expressed genes, which was detected in the crop epithelium, particularly in the apical portions of the cells. Furthermore, we also showed an increase in sugar concentration in a sucrose solution collected from the crop lumen a few minutes after feeding, indicating that water uptake occurs during storage of nectar in the crop, suggesting a previously unidentified function for the honeybee crop.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>24756368</pmid><doi>10.1007/s00709-014-0645-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apis mellifera Aquaporins - genetics Aquaporins - metabolism Bees - anatomy & histology Bees - genetics Bees - metabolism Biomedical and Life Sciences Cell Biology Digestive System - metabolism Epithelium - metabolism Genes, Insect Hierarchy, Social Honey Immunohistochemistry Life Sciences Organ Specificity Original Article Plant Sciences Polymerase Chain Reaction Sucrose - metabolism Zoology |
| title | Aquaporins in the honeybee crop—a new function for an old organ |
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