A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1

A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1

A chemically defined culture medium for the production of S. pneumoniae was developed. The performance of the synthetic medium for cultivation of S. pneumoniae serotype 1 was evaluated by comparing biomass production in this medium with biomass produced by growing the bacteria in trypticase soy brot...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biotech research 2015-01, Vol.6, p.54-54
Hauptverfasser: Texeira, Esther, Checa, Jackeline, Ríal, Analía, Chabalgoity, Jose A, Suárez, Norma
Format: Artikel
Sprache:eng
Schlagworte:
Streptococcus pneumoniae
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 54
container_issue
container_start_page 54
container_title Journal of biotech research
container_volume 6
creator Texeira, Esther
Checa, Jackeline
Ríal, Analía
Chabalgoity, Jose A
Suárez, Norma
description A chemically defined culture medium for the production of S. pneumoniae was developed. The performance of the synthetic medium for cultivation of S. pneumoniae serotype 1 was evaluated by comparing biomass production in this medium with biomass produced by growing the bacteria in trypticase soy broth medium. Bacterial growth was monitored by measuring the optical density, change in the pH of the medium and glucose consumption. Production of capsular polysaccharide was confirmed by using a specific latex reagent in culture supernatant. The performance of the synthetic medium analyzed by matrix-assisted laser desorption/ionization time-of-flight on intact cells showed an improvement of the resolution of samples/matrix mixture and a very homogeneous spectrum which confirmed the feasibility of growth of S. pneumoniae in the chemically defined medium.
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1735920463</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3843311791</sourcerecordid><originalsourceid>FETCH-LOGICAL-p613-e68125cc2fb767ae2411fd145d385827116d8ee949e4c17997efd9ce7f4e012b3</originalsourceid><addsrcrecordid>eNpdjs1KAzEYRQdRsNS-Q8CNm4H58jNJlqX4BwUXLW5LmnzBlJlknEyUvr0juhDv5tzF4XIvqgVozmtGlbj806-rVc6n5juyaZVYVK9rEvGT2DfsgzVddyYOfYjoSI8ulJ74NBJbuil8mCmkSJInu2nEYUo2WVsyGSKWPsVgkOxwTNN5QAI31ZU3XcbVL5fV_uF-v3mqty-Pz5v1th5aYDW2CqiwlvqjbKVBygG8Ay4cU0JRCdA6hai5Rm5Bai3RO21Reo4N0CNbVnc_s8OY3gvm6dCHbLHrTMRU8gEkE5o2vGWzevtPPaUyxvncbFHRAJMK2BdHSVxi</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1725013781</pqid></control><display><type>article</type><title>A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Texeira, Esther ; Checa, Jackeline ; Ríal, Analía ; Chabalgoity, Jose A ; Suárez, Norma</creator><creatorcontrib>Texeira, Esther ; Checa, Jackeline ; Ríal, Analía ; Chabalgoity, Jose A ; Suárez, Norma</creatorcontrib><description>A chemically defined culture medium for the production of S. pneumoniae was developed. The performance of the synthetic medium for cultivation of S. pneumoniae serotype 1 was evaluated by comparing biomass production in this medium with biomass produced by growing the bacteria in trypticase soy broth medium. Bacterial growth was monitored by measuring the optical density, change in the pH of the medium and glucose consumption. Production of capsular polysaccharide was confirmed by using a specific latex reagent in culture supernatant. The performance of the synthetic medium analyzed by matrix-assisted laser desorption/ionization time-of-flight on intact cells showed an improvement of the resolution of samples/matrix mixture and a very homogeneous spectrum which confirmed the feasibility of growth of S. pneumoniae in the chemically defined medium.</description><identifier>ISSN: 1944-3285</identifier><identifier>EISSN: 1944-3285</identifier><language>eng</language><publisher>Edmond: Bio Tech System</publisher><subject>Streptococcus pneumoniae</subject><ispartof>Journal of biotech research, 2015-01, Vol.6, p.54-54</ispartof><rights>Copyright Bio Tech System 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Texeira, Esther</creatorcontrib><creatorcontrib>Checa, Jackeline</creatorcontrib><creatorcontrib>Ríal, Analía</creatorcontrib><creatorcontrib>Chabalgoity, Jose A</creatorcontrib><creatorcontrib>Suárez, Norma</creatorcontrib><title>A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1</title><title>Journal of biotech research</title><description>A chemically defined culture medium for the production of S. pneumoniae was developed. The performance of the synthetic medium for cultivation of S. pneumoniae serotype 1 was evaluated by comparing biomass production in this medium with biomass produced by growing the bacteria in trypticase soy broth medium. Bacterial growth was monitored by measuring the optical density, change in the pH of the medium and glucose consumption. Production of capsular polysaccharide was confirmed by using a specific latex reagent in culture supernatant. The performance of the synthetic medium analyzed by matrix-assisted laser desorption/ionization time-of-flight on intact cells showed an improvement of the resolution of samples/matrix mixture and a very homogeneous spectrum which confirmed the feasibility of growth of S. pneumoniae in the chemically defined medium.</description><subject>Streptococcus pneumoniae</subject><issn>1944-3285</issn><issn>1944-3285</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdjs1KAzEYRQdRsNS-Q8CNm4H58jNJlqX4BwUXLW5LmnzBlJlknEyUvr0juhDv5tzF4XIvqgVozmtGlbj806-rVc6n5juyaZVYVK9rEvGT2DfsgzVddyYOfYjoSI8ulJ74NBJbuil8mCmkSJInu2nEYUo2WVsyGSKWPsVgkOxwTNN5QAI31ZU3XcbVL5fV_uF-v3mqty-Pz5v1th5aYDW2CqiwlvqjbKVBygG8Ay4cU0JRCdA6hai5Rm5Bai3RO21Reo4N0CNbVnc_s8OY3gvm6dCHbLHrTMRU8gEkE5o2vGWzevtPPaUyxvncbFHRAJMK2BdHSVxi</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Texeira, Esther</creator><creator>Checa, Jackeline</creator><creator>Ríal, Analía</creator><creator>Chabalgoity, Jose A</creator><creator>Suárez, Norma</creator><general>Bio Tech System</general><scope>3V.</scope><scope>7QO</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20150101</creationdate><title>A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1</title><author>Texeira, Esther ; Checa, Jackeline ; Ríal, Analía ; Chabalgoity, Jose A ; Suárez, Norma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p613-e68125cc2fb767ae2411fd145d385827116d8ee949e4c17997efd9ce7f4e012b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Streptococcus pneumoniae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Texeira, Esther</creatorcontrib><creatorcontrib>Checa, Jackeline</creatorcontrib><creatorcontrib>Ríal, Analía</creatorcontrib><creatorcontrib>Chabalgoity, Jose A</creatorcontrib><creatorcontrib>Suárez, Norma</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of biotech research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Texeira, Esther</au><au>Checa, Jackeline</au><au>Ríal, Analía</au><au>Chabalgoity, Jose A</au><au>Suárez, Norma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1</atitle><jtitle>Journal of biotech research</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>6</volume><spage>54</spage><epage>54</epage><pages>54-54</pages><issn>1944-3285</issn><eissn>1944-3285</eissn><abstract>A chemically defined culture medium for the production of S. pneumoniae was developed. The performance of the synthetic medium for cultivation of S. pneumoniae serotype 1 was evaluated by comparing biomass production in this medium with biomass produced by growing the bacteria in trypticase soy broth medium. Bacterial growth was monitored by measuring the optical density, change in the pH of the medium and glucose consumption. Production of capsular polysaccharide was confirmed by using a specific latex reagent in culture supernatant. The performance of the synthetic medium analyzed by matrix-assisted laser desorption/ionization time-of-flight on intact cells showed an improvement of the resolution of samples/matrix mixture and a very homogeneous spectrum which confirmed the feasibility of growth of S. pneumoniae in the chemically defined medium.</abstract><cop>Edmond</cop><pub>Bio Tech System</pub><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1944-3285
ispartof Journal of biotech research, 2015-01, Vol.6, p.54-54
issn 1944-3285
1944-3285
language eng
recordid cdi_proquest_miscellaneous_1735920463
source EZB-FREE-00999 freely available EZB journals
subjects Streptococcus pneumoniae
title A new chemically defined medium for cultivation of Streptococcus pneumoniae Serotype 1
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A55%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20new%20chemically%20defined%20medium%20for%20cultivation%20of%20Streptococcus%20pneumoniae%20Serotype%201&rft.jtitle=Journal%20of%20biotech%20research&rft.au=Texeira,%20Esther&rft.date=2015-01-01&rft.volume=6&rft.spage=54&rft.epage=54&rft.pages=54-54&rft.issn=1944-3285&rft.eissn=1944-3285&rft_id=info:doi/&rft_dat=%3Cproquest%3E3843311791%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1725013781&rft_id=info:pmid/&rfr_iscdi=true