Comparison of orthologous cytochrome P450 genes relative expression patterns in the bark beetles Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae) during host colonization
Bark beetles of the genus Dendroctonus are important components of coniferous forests. During host colonization, they must overcome the chemical defences of their host trees, which are metabolized by cytochrome P450 (CYP or P450) enzymes to compounds that are readily excreted. In this study, we repo...
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| Veröffentlicht in: | Insect molecular biology 2015-12, Vol.24 (6), p.649-661 |
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| creator | Obregón‐Molina, G Cesar‐Ayala, A. K López, M. F Cano‐Ramírez, C Zúñiga, G |
| description | Bark beetles of the genus Dendroctonus are important components of coniferous forests. During host colonization, they must overcome the chemical defences of their host trees, which are metabolized by cytochrome P450 (CYP or P450) enzymes to compounds that are readily excreted. In this study, we report the relative expression (quantitative real‐time PCR) of four orthologous cytochrome P450 genes (CYP6BW5, CYP6DG1, CYP6DJ2 and CYP9Z20) in Dendroctonus rhizophagus and Dendroctonus valens forced to attack host trees at 8 and 24 h following forced attack and in four stages during natural colonization [solitary females boring the bark (T1); both male and female members of couples before oviposition (T2); both male and female members of couples during oviposition (T3), and solitary females inside the gallery containing eggs (T4)]. For both species gene expression was different compared with that observed in insects exposed to single monoterpenes in the laboratory, and the expression patterns were significantly different amongst species, sex, gut region and exposure time or natural colonization stage. The induction of genes (CYP6BW5v1, CYP6DJ2v1 and CYP9Z20v1 from D. rhizophagus, as well as CYP6DG1v3 from D. valens) correlated with colonization stage as well as with the increase in oxygenated monoterpenes in the gut of both species throughout the colonization of the host. Our results point to different functions of these orthologous genes in both species. |
| doi_str_mv | 10.1111/imb.12191 |
| format | Article |
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K ; López, M. F ; Cano‐Ramírez, C ; Zúñiga, G</creator><creatorcontrib>Obregón‐Molina, G ; Cesar‐Ayala, A. K ; López, M. F ; Cano‐Ramírez, C ; Zúñiga, G</creatorcontrib><description>Bark beetles of the genus Dendroctonus are important components of coniferous forests. During host colonization, they must overcome the chemical defences of their host trees, which are metabolized by cytochrome P450 (CYP or P450) enzymes to compounds that are readily excreted. In this study, we report the relative expression (quantitative real‐time PCR) of four orthologous cytochrome P450 genes (CYP6BW5, CYP6DG1, CYP6DJ2 and CYP9Z20) in Dendroctonus rhizophagus and Dendroctonus valens forced to attack host trees at 8 and 24 h following forced attack and in four stages during natural colonization [solitary females boring the bark (T1); both male and female members of couples before oviposition (T2); both male and female members of couples during oviposition (T3), and solitary females inside the gallery containing eggs (T4)]. For both species gene expression was different compared with that observed in insects exposed to single monoterpenes in the laboratory, and the expression patterns were significantly different amongst species, sex, gut region and exposure time or natural colonization stage. The induction of genes (CYP6BW5v1, CYP6DJ2v1 and CYP9Z20v1 from D. rhizophagus, as well as CYP6DG1v3 from D. valens) correlated with colonization stage as well as with the increase in oxygenated monoterpenes in the gut of both species throughout the colonization of the host. Our results point to different functions of these orthologous genes in both species.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/imb.12191</identifier><identifier>PMID: 26537737</identifier><language>eng</language><publisher>England: Published for the Royal Entomological Society by Blackwell Scientific Publications</publisher><subject>Animals ; bark ; bark beetles ; coniferous forests ; Curculionidae ; cytochrome genes ; cytochrome P-450 ; Cytochrome P-450 Enzyme System - chemistry ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Dendroctonus ; Dendroctonus valens ; detoxification ; digestive system ; eggs ; enzymes ; exposure duration ; Female ; females ; Gastrointestinal Tract - enzymology ; gene expression ; Gene Expression Regulation ; genes ; Male ; males ; Mexico ; monoterpenes ; Monoterpenes - metabolism ; monoterpenoids ; Oviposition ; Pinus - metabolism ; Pinus - parasitology ; quantitative polymerase chain reaction ; Rhizophagus ; Scolytidae ; Scolytinae ; Time Factors ; trees ; Weevils - enzymology ; Weevils - genetics</subject><ispartof>Insect molecular biology, 2015-12, Vol.24 (6), p.649-661</ispartof><rights>2015 The Royal Entomological Society</rights><rights>2015 The Royal Entomological Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5181-43b7a6627e6e01b15987ee45faf7069f6e1d7be4256f6a97f7dd914e616cdce3</citedby><cites>FETCH-LOGICAL-c5181-43b7a6627e6e01b15987ee45faf7069f6e1d7be4256f6a97f7dd914e616cdce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fimb.12191$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fimb.12191$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26537737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Obregón‐Molina, G</creatorcontrib><creatorcontrib>Cesar‐Ayala, A. K</creatorcontrib><creatorcontrib>López, M. F</creatorcontrib><creatorcontrib>Cano‐Ramírez, C</creatorcontrib><creatorcontrib>Zúñiga, G</creatorcontrib><title>Comparison of orthologous cytochrome P450 genes relative expression patterns in the bark beetles Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae) during host colonization</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>Bark beetles of the genus Dendroctonus are important components of coniferous forests. During host colonization, they must overcome the chemical defences of their host trees, which are metabolized by cytochrome P450 (CYP or P450) enzymes to compounds that are readily excreted. In this study, we report the relative expression (quantitative real‐time PCR) of four orthologous cytochrome P450 genes (CYP6BW5, CYP6DG1, CYP6DJ2 and CYP9Z20) in Dendroctonus rhizophagus and Dendroctonus valens forced to attack host trees at 8 and 24 h following forced attack and in four stages during natural colonization [solitary females boring the bark (T1); both male and female members of couples before oviposition (T2); both male and female members of couples during oviposition (T3), and solitary females inside the gallery containing eggs (T4)]. For both species gene expression was different compared with that observed in insects exposed to single monoterpenes in the laboratory, and the expression patterns were significantly different amongst species, sex, gut region and exposure time or natural colonization stage. The induction of genes (CYP6BW5v1, CYP6DJ2v1 and CYP9Z20v1 from D. rhizophagus, as well as CYP6DG1v3 from D. valens) correlated with colonization stage as well as with the increase in oxygenated monoterpenes in the gut of both species throughout the colonization of the host. Our results point to different functions of these orthologous genes in both species.</description><subject>Animals</subject><subject>bark</subject><subject>bark beetles</subject><subject>coniferous forests</subject><subject>Curculionidae</subject><subject>cytochrome genes</subject><subject>cytochrome P-450</subject><subject>Cytochrome P-450 Enzyme System - chemistry</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Dendroctonus</subject><subject>Dendroctonus valens</subject><subject>detoxification</subject><subject>digestive system</subject><subject>eggs</subject><subject>enzymes</subject><subject>exposure duration</subject><subject>Female</subject><subject>females</subject><subject>Gastrointestinal Tract - enzymology</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>Male</subject><subject>males</subject><subject>Mexico</subject><subject>monoterpenes</subject><subject>Monoterpenes - metabolism</subject><subject>monoterpenoids</subject><subject>Oviposition</subject><subject>Pinus - metabolism</subject><subject>Pinus - parasitology</subject><subject>quantitative polymerase chain reaction</subject><subject>Rhizophagus</subject><subject>Scolytidae</subject><subject>Scolytinae</subject><subject>Time Factors</subject><subject>trees</subject><subject>Weevils - enzymology</subject><subject>Weevils - genetics</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1u1DAURiMEoqWw4AXAEpt2kdbOjx2zowN0KpWC1KJKbCwnuZm4JHawndLpA_JcXJi2EkhIeGPLPvfI9v2S5Dmj-wzHgRnrfZYxyR4k2yznZZqVVf4w2aaSZymjotxKnoRwSSmtJJePk62Ml7kQudhOfizcOGlvgrPEdcT52LvBrdwcSLOOrum9G4F8KkpKVmAhEA-DjuYKCFxPHkIwWDjpGMHbQIwlsQdSa_-V1ABxwIK3YFvvmugsOn1vbtzU6xWutW3_PLzSA6BkdzH7Zh5QbFoNr8lZ44Z1NFbDHmlnb-yK9C5EgtuI3OBtnH2aPOr0EODZ7byTnL9_d75Ypicfj44Xb07SpmQVS4u8FprzTAAHympWykoAFGWnO0G57DiwVtRQZCXvuJaiE20rWQGc8aZtIN9JdjfaybtvM4SoRhMaGAZtAb9MMZGXkkkq-P-gTFS8ogzRV3-hl272Ft-BVCYFKyopkdrbUI13IXjo1OTNqP1aMap-xUBhDNTvGCD74tY41yO09-Rd3xE42ADfzQDrf5vU8YfDO2W6qTAhwvV9BXZacTSW6uL0SBUXy8PTBf-ilsi_3PCddkqvMGHq81lGGccUFqKSef4T48PZIw</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Obregón‐Molina, G</creator><creator>Cesar‐Ayala, A. K</creator><creator>López, M. F</creator><creator>Cano‐Ramírez, C</creator><creator>Zúñiga, G</creator><general>Published for the Royal Entomological Society by Blackwell Scientific Publications</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201512</creationdate><title>Comparison of orthologous cytochrome P450 genes relative expression patterns in the bark beetles Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae) during host colonization</title><author>Obregón‐Molina, G ; Cesar‐Ayala, A. K ; López, M. 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K</au><au>López, M. F</au><au>Cano‐Ramírez, C</au><au>Zúñiga, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of orthologous cytochrome P450 genes relative expression patterns in the bark beetles Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae) during host colonization</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2015-12</date><risdate>2015</risdate><volume>24</volume><issue>6</issue><spage>649</spage><epage>661</epage><pages>649-661</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>Bark beetles of the genus Dendroctonus are important components of coniferous forests. During host colonization, they must overcome the chemical defences of their host trees, which are metabolized by cytochrome P450 (CYP or P450) enzymes to compounds that are readily excreted. In this study, we report the relative expression (quantitative real‐time PCR) of four orthologous cytochrome P450 genes (CYP6BW5, CYP6DG1, CYP6DJ2 and CYP9Z20) in Dendroctonus rhizophagus and Dendroctonus valens forced to attack host trees at 8 and 24 h following forced attack and in four stages during natural colonization [solitary females boring the bark (T1); both male and female members of couples before oviposition (T2); both male and female members of couples during oviposition (T3), and solitary females inside the gallery containing eggs (T4)]. For both species gene expression was different compared with that observed in insects exposed to single monoterpenes in the laboratory, and the expression patterns were significantly different amongst species, sex, gut region and exposure time or natural colonization stage. The induction of genes (CYP6BW5v1, CYP6DJ2v1 and CYP9Z20v1 from D. rhizophagus, as well as CYP6DG1v3 from D. valens) correlated with colonization stage as well as with the increase in oxygenated monoterpenes in the gut of both species throughout the colonization of the host. Our results point to different functions of these orthologous genes in both species.</abstract><cop>England</cop><pub>Published for the Royal Entomological Society by Blackwell Scientific Publications</pub><pmid>26537737</pmid><doi>10.1111/imb.12191</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals bark bark beetles coniferous forests Curculionidae cytochrome genes cytochrome P-450 Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Dendroctonus Dendroctonus valens detoxification digestive system eggs enzymes exposure duration Female females Gastrointestinal Tract - enzymology gene expression Gene Expression Regulation genes Male males Mexico monoterpenes Monoterpenes - metabolism monoterpenoids Oviposition Pinus - metabolism Pinus - parasitology quantitative polymerase chain reaction Rhizophagus Scolytidae Scolytinae Time Factors trees Weevils - enzymology Weevils - genetics |
| title | Comparison of orthologous cytochrome P450 genes relative expression patterns in the bark beetles Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae) during host colonization |
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