Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system
Abstract Background and aims Aggregation of alpha-synuclein (a-syn) has been implicated in the development of neurodegenerative diseases including its spread from the enteric nervous system (ENS) to the brain. Physiologically, a-syn is located at the presynapse and might be involved in regulating of...
Gespeichert in:
| Veröffentlicht in: | Brain research 2015-07, Vol.1614, p.51-59 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 59 |
|---|---|
| container_issue | |
| container_start_page | 51 |
| container_title | Brain research |
| container_volume | 1614 |
| creator | Böttner, Martina Fricke, Tobias Müller, Melanie Barrenschee, Martina Deuschl, Günther Schneider, Susanne A Egberts, Jan-Hendrik Becker, Thomas Fritscher-Ravens, Annette Ellrichmann, Mark Schulz-Schaeffer, Walter J Wedel, Thilo |
| description | Abstract Background and aims Aggregation of alpha-synuclein (a-syn) has been implicated in the development of neurodegenerative diseases including its spread from the enteric nervous system (ENS) to the brain. Physiologically, a-syn is located at the presynapse and might be involved in regulating of neurotransmission. Therefore, the aim of the study was to characterize the physiological ontogenetic and locoregional expression pattern of a-syn in the ENS and its association with the synaptic vesicle apparatus. Material and methods Ontogenetic mRNA expression of a-syn and synaptophysin was determined in the rat intestine. Myenteric plexus cultures treated with glial cell line-derived neurotrophic factor (GDNF) were assessed for mRNA expression of a-syn, co-localization of a-syn with the pan-neuronal marker PGP 9.5 and the synaptic vesicle marker synaptophysin and studied by scanning electron microscopy (SEM). Human colonic specimens were subjected to co-localization studies of a-syn with synaptophysin. Results a-syn and synaptophysin intestinal gene expression levels were highest during early postnatal life and also detectable at adult age. a-syn was co-localized with PGP 9.5 and synaptophysin in myenteric plexus cultures and up-regulated after GDNF treatment. SEM confirmed the presence of neuronal varicosities to which a-syn was associated. Consistently, a-syn and synaptophysin showed partial co-localization in the human ENS. Conclusions The ontogenetic and cellular expression pattern as well as the regulation by GNDF give evidence that a-syn is physiologically associated to the synaptic vesicle apparatus. The data suggest that a-syn is involved in the regulation of synaptic plasticity in the ENS during early postnatal life and adult age. |
| doi_str_mv | 10.1016/j.brainres.2015.04.015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1735913812</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006899315003042</els_id><sourcerecordid>1735913812</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-72ab82a2ed1fce066ab3a5e3905fff443f2cb188bb5d33d4daf7973bf25641b83</originalsourceid><addsrcrecordid>eNqFkk9v1DAQxS1ERZfCV6h85JLgP4njXBBVRQGpEgfK2Zo4E62XxAm2s2i_fR1ty4FLT0-2fvNmNG8Iueas5Iyrj4eyC-B8wFgKxuuSVWWWV2THdSMKJSr2muwYY6rQbSsvydsYD_kpZcvekEtR61a1st2R6WZc9lDEk1_tiM5TFynEOFsHCXv616U9TXukGYAlOUuPGF0mKSwLBEhrpLloI_brBJ6C72n-pugThox7DMc5Q_EUE07vyMUAY8T3T3pFft19ebj9Vtz_-Pr99ua-sLVQqWgEdFqAwJ4PFplS0EmoMc9eD8NQVXIQtuNad13dS9lXPQxN28huELWqeKflFflw9l3C_GfFmMzkosVxBI95GsMbWbdcai5eRpXOno1mKqPqjNowxxhwMEtwE4ST4cxsqZiDeU7FbKkYVpksufD6qcfaTdj_K3uOIQOfzwDmpRwdBhOtQ2-xdwFtMv3sXu7x6T8LOzrvLIy_8YTxMK_B55UbbqIwzPzcbmM7DV7nq2CVkI8br7hU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1686417806</pqid></control><display><type>article</type><title>Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Böttner, Martina ; Fricke, Tobias ; Müller, Melanie ; Barrenschee, Martina ; Deuschl, Günther ; Schneider, Susanne A ; Egberts, Jan-Hendrik ; Becker, Thomas ; Fritscher-Ravens, Annette ; Ellrichmann, Mark ; Schulz-Schaeffer, Walter J ; Wedel, Thilo</creator><creatorcontrib>Böttner, Martina ; Fricke, Tobias ; Müller, Melanie ; Barrenschee, Martina ; Deuschl, Günther ; Schneider, Susanne A ; Egberts, Jan-Hendrik ; Becker, Thomas ; Fritscher-Ravens, Annette ; Ellrichmann, Mark ; Schulz-Schaeffer, Walter J ; Wedel, Thilo</creatorcontrib><description>Abstract Background and aims Aggregation of alpha-synuclein (a-syn) has been implicated in the development of neurodegenerative diseases including its spread from the enteric nervous system (ENS) to the brain. Physiologically, a-syn is located at the presynapse and might be involved in regulating of neurotransmission. Therefore, the aim of the study was to characterize the physiological ontogenetic and locoregional expression pattern of a-syn in the ENS and its association with the synaptic vesicle apparatus. Material and methods Ontogenetic mRNA expression of a-syn and synaptophysin was determined in the rat intestine. Myenteric plexus cultures treated with glial cell line-derived neurotrophic factor (GDNF) were assessed for mRNA expression of a-syn, co-localization of a-syn with the pan-neuronal marker PGP 9.5 and the synaptic vesicle marker synaptophysin and studied by scanning electron microscopy (SEM). Human colonic specimens were subjected to co-localization studies of a-syn with synaptophysin. Results a-syn and synaptophysin intestinal gene expression levels were highest during early postnatal life and also detectable at adult age. a-syn was co-localized with PGP 9.5 and synaptophysin in myenteric plexus cultures and up-regulated after GDNF treatment. SEM confirmed the presence of neuronal varicosities to which a-syn was associated. Consistently, a-syn and synaptophysin showed partial co-localization in the human ENS. Conclusions The ontogenetic and cellular expression pattern as well as the regulation by GNDF give evidence that a-syn is physiologically associated to the synaptic vesicle apparatus. The data suggest that a-syn is involved in the regulation of synaptic plasticity in the ENS during early postnatal life and adult age.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2015.04.015</identifier><identifier>PMID: 25896939</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Age Factors ; Aged ; Alpha synuclein ; alpha-Synuclein - genetics ; alpha-Synuclein - metabolism ; Animals ; Animals, Newborn ; Cells, Cultured ; Enteric nerve cell culture ; Enteric nervous system ; Enteric Nervous System - cytology ; Enteric Nervous System - growth & development ; Enteric Nervous System - metabolism ; Gene Expression Regulation - drug effects ; Glial Cell Line-Derived Neurotrophic Factor - pharmacology ; Human colon ; Humans ; In Vitro Techniques ; Male ; Neurology ; Neurons - cytology ; Neurons - drug effects ; Rats ; RNA, Messenger - metabolism ; Statistics, Nonparametric ; Synaptic vesicles ; Synaptic Vesicles - metabolism ; Synaptophysin - metabolism ; Ubiquitin Thiolesterase - metabolism</subject><ispartof>Brain research, 2015-07, Vol.1614, p.51-59</ispartof><rights>Elsevier B.V.</rights><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-72ab82a2ed1fce066ab3a5e3905fff443f2cb188bb5d33d4daf7973bf25641b83</citedby><cites>FETCH-LOGICAL-c526t-72ab82a2ed1fce066ab3a5e3905fff443f2cb188bb5d33d4daf7973bf25641b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2015.04.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25896939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Böttner, Martina</creatorcontrib><creatorcontrib>Fricke, Tobias</creatorcontrib><creatorcontrib>Müller, Melanie</creatorcontrib><creatorcontrib>Barrenschee, Martina</creatorcontrib><creatorcontrib>Deuschl, Günther</creatorcontrib><creatorcontrib>Schneider, Susanne A</creatorcontrib><creatorcontrib>Egberts, Jan-Hendrik</creatorcontrib><creatorcontrib>Becker, Thomas</creatorcontrib><creatorcontrib>Fritscher-Ravens, Annette</creatorcontrib><creatorcontrib>Ellrichmann, Mark</creatorcontrib><creatorcontrib>Schulz-Schaeffer, Walter J</creatorcontrib><creatorcontrib>Wedel, Thilo</creatorcontrib><title>Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract Background and aims Aggregation of alpha-synuclein (a-syn) has been implicated in the development of neurodegenerative diseases including its spread from the enteric nervous system (ENS) to the brain. Physiologically, a-syn is located at the presynapse and might be involved in regulating of neurotransmission. Therefore, the aim of the study was to characterize the physiological ontogenetic and locoregional expression pattern of a-syn in the ENS and its association with the synaptic vesicle apparatus. Material and methods Ontogenetic mRNA expression of a-syn and synaptophysin was determined in the rat intestine. Myenteric plexus cultures treated with glial cell line-derived neurotrophic factor (GDNF) were assessed for mRNA expression of a-syn, co-localization of a-syn with the pan-neuronal marker PGP 9.5 and the synaptic vesicle marker synaptophysin and studied by scanning electron microscopy (SEM). Human colonic specimens were subjected to co-localization studies of a-syn with synaptophysin. Results a-syn and synaptophysin intestinal gene expression levels were highest during early postnatal life and also detectable at adult age. a-syn was co-localized with PGP 9.5 and synaptophysin in myenteric plexus cultures and up-regulated after GDNF treatment. SEM confirmed the presence of neuronal varicosities to which a-syn was associated. Consistently, a-syn and synaptophysin showed partial co-localization in the human ENS. Conclusions The ontogenetic and cellular expression pattern as well as the regulation by GNDF give evidence that a-syn is physiologically associated to the synaptic vesicle apparatus. The data suggest that a-syn is involved in the regulation of synaptic plasticity in the ENS during early postnatal life and adult age.</description><subject>Age Factors</subject><subject>Aged</subject><subject>Alpha synuclein</subject><subject>alpha-Synuclein - genetics</subject><subject>alpha-Synuclein - metabolism</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Cells, Cultured</subject><subject>Enteric nerve cell culture</subject><subject>Enteric nervous system</subject><subject>Enteric Nervous System - cytology</subject><subject>Enteric Nervous System - growth & development</subject><subject>Enteric Nervous System - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glial Cell Line-Derived Neurotrophic Factor - pharmacology</subject><subject>Human colon</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Neurology</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>Statistics, Nonparametric</subject><subject>Synaptic vesicles</subject><subject>Synaptic Vesicles - metabolism</subject><subject>Synaptophysin - metabolism</subject><subject>Ubiquitin Thiolesterase - metabolism</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9v1DAQxS1ERZfCV6h85JLgP4njXBBVRQGpEgfK2Zo4E62XxAm2s2i_fR1ty4FLT0-2fvNmNG8Iueas5Iyrj4eyC-B8wFgKxuuSVWWWV2THdSMKJSr2muwYY6rQbSsvydsYD_kpZcvekEtR61a1st2R6WZc9lDEk1_tiM5TFynEOFsHCXv616U9TXukGYAlOUuPGF0mKSwLBEhrpLloI_brBJ6C72n-pugThox7DMc5Q_EUE07vyMUAY8T3T3pFft19ebj9Vtz_-Pr99ua-sLVQqWgEdFqAwJ4PFplS0EmoMc9eD8NQVXIQtuNad13dS9lXPQxN28huELWqeKflFflw9l3C_GfFmMzkosVxBI95GsMbWbdcai5eRpXOno1mKqPqjNowxxhwMEtwE4ST4cxsqZiDeU7FbKkYVpksufD6qcfaTdj_K3uOIQOfzwDmpRwdBhOtQ2-xdwFtMv3sXu7x6T8LOzrvLIy_8YTxMK_B55UbbqIwzPzcbmM7DV7nq2CVkI8br7hU</recordid><startdate>20150721</startdate><enddate>20150721</enddate><creator>Böttner, Martina</creator><creator>Fricke, Tobias</creator><creator>Müller, Melanie</creator><creator>Barrenschee, Martina</creator><creator>Deuschl, Günther</creator><creator>Schneider, Susanne A</creator><creator>Egberts, Jan-Hendrik</creator><creator>Becker, Thomas</creator><creator>Fritscher-Ravens, Annette</creator><creator>Ellrichmann, Mark</creator><creator>Schulz-Schaeffer, Walter J</creator><creator>Wedel, Thilo</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20150721</creationdate><title>Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system</title><author>Böttner, Martina ; Fricke, Tobias ; Müller, Melanie ; Barrenschee, Martina ; Deuschl, Günther ; Schneider, Susanne A ; Egberts, Jan-Hendrik ; Becker, Thomas ; Fritscher-Ravens, Annette ; Ellrichmann, Mark ; Schulz-Schaeffer, Walter J ; Wedel, Thilo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-72ab82a2ed1fce066ab3a5e3905fff443f2cb188bb5d33d4daf7973bf25641b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Age Factors</topic><topic>Aged</topic><topic>Alpha synuclein</topic><topic>alpha-Synuclein - genetics</topic><topic>alpha-Synuclein - metabolism</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Cells, Cultured</topic><topic>Enteric nerve cell culture</topic><topic>Enteric nervous system</topic><topic>Enteric Nervous System - cytology</topic><topic>Enteric Nervous System - growth & development</topic><topic>Enteric Nervous System - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glial Cell Line-Derived Neurotrophic Factor - pharmacology</topic><topic>Human colon</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Neurology</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>Statistics, Nonparametric</topic><topic>Synaptic vesicles</topic><topic>Synaptic Vesicles - metabolism</topic><topic>Synaptophysin - metabolism</topic><topic>Ubiquitin Thiolesterase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Böttner, Martina</creatorcontrib><creatorcontrib>Fricke, Tobias</creatorcontrib><creatorcontrib>Müller, Melanie</creatorcontrib><creatorcontrib>Barrenschee, Martina</creatorcontrib><creatorcontrib>Deuschl, Günther</creatorcontrib><creatorcontrib>Schneider, Susanne A</creatorcontrib><creatorcontrib>Egberts, Jan-Hendrik</creatorcontrib><creatorcontrib>Becker, Thomas</creatorcontrib><creatorcontrib>Fritscher-Ravens, Annette</creatorcontrib><creatorcontrib>Ellrichmann, Mark</creatorcontrib><creatorcontrib>Schulz-Schaeffer, Walter J</creatorcontrib><creatorcontrib>Wedel, Thilo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Böttner, Martina</au><au>Fricke, Tobias</au><au>Müller, Melanie</au><au>Barrenschee, Martina</au><au>Deuschl, Günther</au><au>Schneider, Susanne A</au><au>Egberts, Jan-Hendrik</au><au>Becker, Thomas</au><au>Fritscher-Ravens, Annette</au><au>Ellrichmann, Mark</au><au>Schulz-Schaeffer, Walter J</au><au>Wedel, Thilo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2015-07-21</date><risdate>2015</risdate><volume>1614</volume><spage>51</spage><epage>59</epage><pages>51-59</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>Abstract Background and aims Aggregation of alpha-synuclein (a-syn) has been implicated in the development of neurodegenerative diseases including its spread from the enteric nervous system (ENS) to the brain. Physiologically, a-syn is located at the presynapse and might be involved in regulating of neurotransmission. Therefore, the aim of the study was to characterize the physiological ontogenetic and locoregional expression pattern of a-syn in the ENS and its association with the synaptic vesicle apparatus. Material and methods Ontogenetic mRNA expression of a-syn and synaptophysin was determined in the rat intestine. Myenteric plexus cultures treated with glial cell line-derived neurotrophic factor (GDNF) were assessed for mRNA expression of a-syn, co-localization of a-syn with the pan-neuronal marker PGP 9.5 and the synaptic vesicle marker synaptophysin and studied by scanning electron microscopy (SEM). Human colonic specimens were subjected to co-localization studies of a-syn with synaptophysin. Results a-syn and synaptophysin intestinal gene expression levels were highest during early postnatal life and also detectable at adult age. a-syn was co-localized with PGP 9.5 and synaptophysin in myenteric plexus cultures and up-regulated after GDNF treatment. SEM confirmed the presence of neuronal varicosities to which a-syn was associated. Consistently, a-syn and synaptophysin showed partial co-localization in the human ENS. Conclusions The ontogenetic and cellular expression pattern as well as the regulation by GNDF give evidence that a-syn is physiologically associated to the synaptic vesicle apparatus. The data suggest that a-syn is involved in the regulation of synaptic plasticity in the ENS during early postnatal life and adult age.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25896939</pmid><doi>10.1016/j.brainres.2015.04.015</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-8993 |
| ispartof | Brain research, 2015-07, Vol.1614, p.51-59 |
| issn | 0006-8993 1872-6240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1735913812 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Age Factors Aged Alpha synuclein alpha-Synuclein - genetics alpha-Synuclein - metabolism Animals Animals, Newborn Cells, Cultured Enteric nerve cell culture Enteric nervous system Enteric Nervous System - cytology Enteric Nervous System - growth & development Enteric Nervous System - metabolism Gene Expression Regulation - drug effects Glial Cell Line-Derived Neurotrophic Factor - pharmacology Human colon Humans In Vitro Techniques Male Neurology Neurons - cytology Neurons - drug effects Rats RNA, Messenger - metabolism Statistics, Nonparametric Synaptic vesicles Synaptic Vesicles - metabolism Synaptophysin - metabolism Ubiquitin Thiolesterase - metabolism |
| title | Alpha-synuclein is associated with the synaptic vesicle apparatus in the human and rat enteric nervous system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T16%3A30%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Alpha-synuclein%20is%20associated%20with%20the%20synaptic%20vesicle%20apparatus%20in%20the%20human%20and%20rat%20enteric%20nervous%20system&rft.jtitle=Brain%20research&rft.au=B%C3%B6ttner,%20Martina&rft.date=2015-07-21&rft.volume=1614&rft.spage=51&rft.epage=59&rft.pages=51-59&rft.issn=0006-8993&rft.eissn=1872-6240&rft_id=info:doi/10.1016/j.brainres.2015.04.015&rft_dat=%3Cproquest_cross%3E1735913812%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1686417806&rft_id=info:pmid/25896939&rft_els_id=S0006899315003042&rfr_iscdi=true |