A protocol for chemical mutagenesis in Strongyloides ratti
•We present an improved protocol for chemical mutagenesis of Strongyloides ratti.•Relatively high genetic variability complicates the identification of mutations.•Further inbreeding of S. ratti is desirable and tolerated by the organism. Genetic analysis using experimentally induced mutations has be...
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| Veröffentlicht in: | Experimental parasitology 2015-11, Vol.158, p.2-7 |
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| container_title | Experimental parasitology |
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| creator | Guo, Li Chang, Zisong Dieterich, Christoph Streit, Adrian |
| description | •We present an improved protocol for chemical mutagenesis of Strongyloides ratti.•Relatively high genetic variability complicates the identification of mutations.•Further inbreeding of S. ratti is desirable and tolerated by the organism.
Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing. |
| doi_str_mv | 10.1016/j.exppara.2015.03.001 |
| format | Article |
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Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2015.03.001</identifier><identifier>PMID: 25765558</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Ethyl Methanesulfonate - pharmacology ; Feasibility Studies ; Female ; Genome, Helminth - drug effects ; Genome, Helminth - physiology ; Genome-Wide Association Study ; Mutagenesis ; Mutagenesis - drug effects ; Mutagenesis - physiology ; Mutagens - pharmacology ; Rats ; Rats, Wistar ; Selection lines ; Sequence Analysis, DNA ; Strongyloides ratti ; Strongyloides ratti - drug effects ; Strongyloides ratti - genetics ; Whole genome re-sequencing</subject><ispartof>Experimental parasitology, 2015-11, Vol.158, p.2-7</ispartof><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-ab0e6d0e68691738bf060469541376dc74b6de2ab450efd790dde664f266591b3</citedby><cites>FETCH-LOGICAL-c435t-ab0e6d0e68691738bf060469541376dc74b6de2ab450efd790dde664f266591b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014489415000545$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25765558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Li</creatorcontrib><creatorcontrib>Chang, Zisong</creatorcontrib><creatorcontrib>Dieterich, Christoph</creatorcontrib><creatorcontrib>Streit, Adrian</creatorcontrib><title>A protocol for chemical mutagenesis in Strongyloides ratti</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>•We present an improved protocol for chemical mutagenesis of Strongyloides ratti.•Relatively high genetic variability complicates the identification of mutations.•Further inbreeding of S. ratti is desirable and tolerated by the organism.
Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.</description><subject>Animals</subject><subject>Ethyl Methanesulfonate - pharmacology</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Genome, Helminth - drug effects</subject><subject>Genome, Helminth - physiology</subject><subject>Genome-Wide Association Study</subject><subject>Mutagenesis</subject><subject>Mutagenesis - drug effects</subject><subject>Mutagenesis - physiology</subject><subject>Mutagens - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Selection lines</subject><subject>Sequence Analysis, DNA</subject><subject>Strongyloides ratti</subject><subject>Strongyloides ratti - drug effects</subject><subject>Strongyloides ratti - genetics</subject><subject>Whole genome re-sequencing</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAQgC0EglL4CaCMLAnnxHYSFoQqXlIlBmC2HPtSXCVxsVNE_z2uWlgZTiedvnt9hFxQyChQcb3M8Hu1Ul5lOVCeQZEB0AMyoVBDmjNWH5JJrLCUVTU7IachLAGgojk7Jic5LwXnvJqQm7tk5d3otOuS1vlEf2BvteqSfj2qBQ4YbEjskLyO3g2LTeeswZB4NY72jBy1qgt4vs9T8v5w_zZ7Sucvj8-zu3mqWcHHVDWAwsSoRE3LompaEMBEzRktSmF0yRphMFcN44CtKWswBoVgbS4Er2lTTMnVbm489HONYZS9DRq7Tg3o1kHGoZEDzvKI8h2qvQvBYytX3vbKbyQFudUml3KvTW61SShklBT7Lvcr1k2P5q_r11MEbncAxke_LHoZtMVBo7Ee9SiNs_-s-AHXXIA_</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Guo, Li</creator><creator>Chang, Zisong</creator><creator>Dieterich, Christoph</creator><creator>Streit, Adrian</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151101</creationdate><title>A protocol for chemical mutagenesis in Strongyloides ratti</title><author>Guo, Li ; Chang, Zisong ; Dieterich, Christoph ; Streit, Adrian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-ab0e6d0e68691738bf060469541376dc74b6de2ab450efd790dde664f266591b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Ethyl Methanesulfonate - pharmacology</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Genome, Helminth - drug effects</topic><topic>Genome, Helminth - physiology</topic><topic>Genome-Wide Association Study</topic><topic>Mutagenesis</topic><topic>Mutagenesis - drug effects</topic><topic>Mutagenesis - physiology</topic><topic>Mutagens - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Selection lines</topic><topic>Sequence Analysis, DNA</topic><topic>Strongyloides ratti</topic><topic>Strongyloides ratti - drug effects</topic><topic>Strongyloides ratti - genetics</topic><topic>Whole genome re-sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Li</creatorcontrib><creatorcontrib>Chang, Zisong</creatorcontrib><creatorcontrib>Dieterich, Christoph</creatorcontrib><creatorcontrib>Streit, Adrian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Li</au><au>Chang, Zisong</au><au>Dieterich, Christoph</au><au>Streit, Adrian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A protocol for chemical mutagenesis in Strongyloides ratti</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>158</volume><spage>2</spage><epage>7</epage><pages>2-7</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><abstract>•We present an improved protocol for chemical mutagenesis of Strongyloides ratti.•Relatively high genetic variability complicates the identification of mutations.•Further inbreeding of S. ratti is desirable and tolerated by the organism.
Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25765558</pmid><doi>10.1016/j.exppara.2015.03.001</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental parasitology, 2015-11, Vol.158, p.2-7 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Ethyl Methanesulfonate - pharmacology Feasibility Studies Female Genome, Helminth - drug effects Genome, Helminth - physiology Genome-Wide Association Study Mutagenesis Mutagenesis - drug effects Mutagenesis - physiology Mutagens - pharmacology Rats Rats, Wistar Selection lines Sequence Analysis, DNA Strongyloides ratti Strongyloides ratti - drug effects Strongyloides ratti - genetics Whole genome re-sequencing |
| title | A protocol for chemical mutagenesis in Strongyloides ratti |
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