Structural Mechanism for Coordination of Proofreading and Polymerase Activities in Archaeal DNA Polymerases

A novel mechanism for controlling the proofreading and polymerase activities of archaeal DNA polymerases was studied. The 3′-5′exonuclease (proofreading) activity and PCR performance of the family B DNA polymerase from Thermococcus kodakaraensis KOD1 (previously Pyrococcus kodakaraensis KOD1) were a...

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Veröffentlicht in:	Journal of molecular biology 2005-08, Vol.351 (2), p.291-298
Hauptverfasser:	Kuroita, Toshihiro, Matsumura, Hiroyoshi, Yokota, Naohiko, Kitabayashi, Masao, Hashimoto, Hiroshi, Inoue, Tsuyoshi, Imanaka, Tadayuki, Kai, Yasushi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Archaeal Proteins - chemistry Archaeal Proteins - physiology archaeral DNA polymerases Binding Sites Cattle Crystallography, X-Ray DNA - chemistry DNA-Directed DNA Polymerase - chemistry DNA-Directed DNA Polymerase - physiology domain interface Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Mutation PCR performance Polymerase Chain Reaction proofreading activity Protein Conformation Protein Structure, Tertiary Static Electricity structural change Thermococcus - enzymology
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