Measuring antigen presentation in mouse brain endothelial cells ex vivo and in vitro

Having demonstrated that brain endothelial cells cross-present parasite antigen in mouse experimental cerebral malaria, the authors present ex vivo and in vitro protocols to measure this antigen presentation, generalizable to other disease contexts. We have recently demonstrated that brain endotheli...

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Veröffentlicht in:	Nature protocols 2015-12, Vol.10 (12), p.2016-2026
Hauptverfasser:	Howland, Shanshan W, Gun, Sin Yee, Claser, Carla, Poh, Chek Meng, Rénia, Laurent
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/767/1424 631/250/21/1293 631/250/255/1629 631/378/371 Analytical Chemistry Animals Antigen Presentation Antigens Biological Techniques Brain Brain - blood supply Brain - cytology Brain - immunology Cell Culture Techniques Cell Line Cells, Cultured Computational Biology/Bioinformatics Disease Models, Animal Endothelial cells Endothelial Cells - cytology Endothelial Cells - immunology Endothelium Erythrocytes Extracellular matrix Health aspects Immunologic tests Interferon Life Sciences Lymphocytes Lymphocytes T Major Histocompatibility Complex Malaria Malaria - immunology Measurement Methods Mice Mice, Inbred C57BL Microarrays Microvessels - cytology Microvessels - immunology Organic Chemistry Parasites Plasmodium berghei - immunology protocol Receptors, Antigen, T-Cell - immunology T cell receptors Vector-borne diseases γ-Interferon
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