VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix
Angiogenesis is a complex procedure induced by the secretion of numerous growth factors from endothelial cells. Vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (FGF2), transforming growth factor-β1, 2, 3 (TGFB1, 2, 3), and transforming growth factor-β receptors (TGFBR1, 2...
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| Veröffentlicht in: | Cancer letters 2005-04, Vol.221 (1), p.105-118 |
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| creator | Soufla, Giannoula Sifakis, Stavros Baritaki, Stavroula Zafiropoulos, Alexandros Koumantakis, Eugenios Spandidos, Demetrios A. |
| description | Angiogenesis is a complex procedure induced by the secretion of numerous growth factors from endothelial cells. Vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (FGF2), transforming growth factor-β1, 2, 3 (TGFB1, 2, 3), and transforming growth factor-β receptors (TGFBR1, 2, 3) mRNA expression pattern was evaluated in tissue samples with cervical intraepithelial neoplasia (CIN) and cervical cancer, compared to that of normal cervical tissues, and correlated to the clinical stage of the disease. Transcript levels of the above genes were assessed by RT-PCR analysis in a total of 44 cervical specimens. VEGF, TGFB1, TGFBR1, and FGF2 transcript levels were significantly different in the normal, CIN and cancer specimen groups (
P=0.015, 0.001, 0.008, and 0.029, respectively). Higher TGFBR1 mRNA levels were observed in parallel with increased severity of the lesion, whereas FGF2 exhibited lower transcript levels. A highly significant increase of VEGF mRNA expression was found upon cervical neoplastic transformation (
P |
| doi_str_mv | 10.1016/j.canlet.2004.08.021 |
| format | Article |
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P=0.015, 0.001, 0.008, and 0.029, respectively). Higher TGFBR1 mRNA levels were observed in parallel with increased severity of the lesion, whereas FGF2 exhibited lower transcript levels. A highly significant increase of VEGF mRNA expression was found upon cervical neoplastic transformation (
P<0.0001). High-grade squamous intraepithelial lesions exhibited higher VEGF mRNA levels than low-grade lesions (
P=0.039). TGFBR1 and TGFBR3 receptors demonstrated significant co-expressions with TGFB2 (
P<0.0001), and TGFB1 (
P=0.005 and 0.002, respectively) in normal cervical specimens. However, a disruption of co-expression patterns was observed in the groups of CIN and cancer cases, compared to normal tissues. Our findings show that VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix. The involvement of the examined markers in cervical carcinogenesis is furthermore supported by the observed disruption of their mRNA co-expression patterns.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2004.08.021</identifier><identifier>PMID: 15797633</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Activin Receptors, Type I - genetics ; Adult ; Angiogenesis ; Cell growth ; Cervical cancer ; Cervical intraepithelial neoplasia ; Cervical Intraepithelial Neoplasia - genetics ; Cervix Uteri - metabolism ; Disease ; Female ; Fibroblast Growth Factor 2 - genetics ; Humans ; Middle Aged ; mRNA Expression ; Protein-Serine-Threonine Kinases ; Receptors, Transforming Growth Factor beta - genetics ; Reproductive system ; RNA, Messenger - metabolism ; Rodents ; RT-PCR ; Studies ; Transforming Growth Factor beta - genetics ; Transforming Growth Factor beta1 ; Transforming Growth Factor beta2 ; Uterine Cervical Neoplasms - genetics ; Vascular Endothelial Growth Factors - genetics</subject><ispartof>Cancer letters, 2005-04, Vol.221 (1), p.105-118</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>Copyright Elsevier Limited Apr 18, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-4f07e448354442d53dbe99db32f459d50d6af186425a910850ce688611c02aa33</citedby><cites>FETCH-LOGICAL-c419t-4f07e448354442d53dbe99db32f459d50d6af186425a910850ce688611c02aa33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.canlet.2004.08.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15797633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soufla, Giannoula</creatorcontrib><creatorcontrib>Sifakis, Stavros</creatorcontrib><creatorcontrib>Baritaki, Stavroula</creatorcontrib><creatorcontrib>Zafiropoulos, Alexandros</creatorcontrib><creatorcontrib>Koumantakis, Eugenios</creatorcontrib><creatorcontrib>Spandidos, Demetrios A.</creatorcontrib><title>VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Angiogenesis is a complex procedure induced by the secretion of numerous growth factors from endothelial cells. Vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (FGF2), transforming growth factor-β1, 2, 3 (TGFB1, 2, 3), and transforming growth factor-β receptors (TGFBR1, 2, 3) mRNA expression pattern was evaluated in tissue samples with cervical intraepithelial neoplasia (CIN) and cervical cancer, compared to that of normal cervical tissues, and correlated to the clinical stage of the disease. Transcript levels of the above genes were assessed by RT-PCR analysis in a total of 44 cervical specimens. VEGF, TGFB1, TGFBR1, and FGF2 transcript levels were significantly different in the normal, CIN and cancer specimen groups (
P=0.015, 0.001, 0.008, and 0.029, respectively). Higher TGFBR1 mRNA levels were observed in parallel with increased severity of the lesion, whereas FGF2 exhibited lower transcript levels. A highly significant increase of VEGF mRNA expression was found upon cervical neoplastic transformation (
P<0.0001). High-grade squamous intraepithelial lesions exhibited higher VEGF mRNA levels than low-grade lesions (
P=0.039). TGFBR1 and TGFBR3 receptors demonstrated significant co-expressions with TGFB2 (
P<0.0001), and TGFB1 (
P=0.005 and 0.002, respectively) in normal cervical specimens. However, a disruption of co-expression patterns was observed in the groups of CIN and cancer cases, compared to normal tissues. Our findings show that VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix. The involvement of the examined markers in cervical carcinogenesis is furthermore supported by the observed disruption of their mRNA co-expression patterns.</description><subject>Activin Receptors, Type I - genetics</subject><subject>Adult</subject><subject>Angiogenesis</subject><subject>Cell growth</subject><subject>Cervical cancer</subject><subject>Cervical intraepithelial neoplasia</subject><subject>Cervical Intraepithelial Neoplasia - genetics</subject><subject>Cervix Uteri - metabolism</subject><subject>Disease</subject><subject>Female</subject><subject>Fibroblast Growth Factor 2 - genetics</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>mRNA Expression</subject><subject>Protein-Serine-Threonine Kinases</subject><subject>Receptors, Transforming Growth Factor beta - genetics</subject><subject>Reproductive system</subject><subject>RNA, Messenger - metabolism</subject><subject>Rodents</subject><subject>RT-PCR</subject><subject>Studies</subject><subject>Transforming Growth Factor beta - genetics</subject><subject>Transforming Growth Factor beta1</subject><subject>Transforming Growth Factor beta2</subject><subject>Uterine Cervical Neoplasms - genetics</subject><subject>Vascular Endothelial Growth Factors - genetics</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVGL1DAQx4Mo3nr6DUQCgk_XOmmSNnkRzuO6CofCcfoasunUy9Kma5Ku57e3e7sg-OBTBvL7z0zyI-Q1g5IBq99vS2fDgLmsAEQJqoSKPSErppqqaLSCp2QFHETBFZdn5EVKWwCQopHPyRmTjW5qzldk_n69bi9ou26rC3q3bj8yakP3WN0yOt5-uaT4sIuYkp8CHXCPQ6JuihEHm5H-8vme5nukox38j2BDpjnakPopjjYfIlP_eD9njD4gdRj3_uEledbbIeGr03lOvrXXd1efipuv689XlzeFE0znQvTQoBDLA4QQVSd5t0Gtuw2veiF1J6Grbc9ULSppNQMlwWGtVM2Yg8pazs_Ju2PfXZx-zpiyGX1yOAw24DQnwxpeaS3qBXz7D7id5hiW3QyTIHmttVYLJY6Ui1NKEXuzi3608bdhYA5SzNYcpZiDFAPKLFKW2JtT83kzYvc3dLKwAB-OwPK5uPcYTXIeg8POR3TZdJP__4Q_AuCcrQ</recordid><startdate>20050418</startdate><enddate>20050418</enddate><creator>Soufla, Giannoula</creator><creator>Sifakis, Stavros</creator><creator>Baritaki, Stavroula</creator><creator>Zafiropoulos, Alexandros</creator><creator>Koumantakis, Eugenios</creator><creator>Spandidos, Demetrios A.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20050418</creationdate><title>VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix</title><author>Soufla, Giannoula ; Sifakis, Stavros ; Baritaki, Stavroula ; Zafiropoulos, Alexandros ; Koumantakis, Eugenios ; Spandidos, Demetrios A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-4f07e448354442d53dbe99db32f459d50d6af186425a910850ce688611c02aa33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Activin Receptors, Type I - genetics</topic><topic>Adult</topic><topic>Angiogenesis</topic><topic>Cell growth</topic><topic>Cervical cancer</topic><topic>Cervical intraepithelial neoplasia</topic><topic>Cervical Intraepithelial Neoplasia - genetics</topic><topic>Cervix Uteri - metabolism</topic><topic>Disease</topic><topic>Female</topic><topic>Fibroblast Growth Factor 2 - genetics</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>mRNA Expression</topic><topic>Protein-Serine-Threonine Kinases</topic><topic>Receptors, Transforming Growth Factor beta - genetics</topic><topic>Reproductive system</topic><topic>RNA, Messenger - metabolism</topic><topic>Rodents</topic><topic>RT-PCR</topic><topic>Studies</topic><topic>Transforming Growth Factor beta - genetics</topic><topic>Transforming Growth Factor beta1</topic><topic>Transforming Growth Factor beta2</topic><topic>Uterine Cervical Neoplasms - genetics</topic><topic>Vascular Endothelial Growth Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soufla, Giannoula</creatorcontrib><creatorcontrib>Sifakis, Stavros</creatorcontrib><creatorcontrib>Baritaki, Stavroula</creatorcontrib><creatorcontrib>Zafiropoulos, Alexandros</creatorcontrib><creatorcontrib>Koumantakis, Eugenios</creatorcontrib><creatorcontrib>Spandidos, Demetrios A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soufla, Giannoula</au><au>Sifakis, Stavros</au><au>Baritaki, Stavroula</au><au>Zafiropoulos, Alexandros</au><au>Koumantakis, Eugenios</au><au>Spandidos, Demetrios A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2005-04-18</date><risdate>2005</risdate><volume>221</volume><issue>1</issue><spage>105</spage><epage>118</epage><pages>105-118</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Angiogenesis is a complex procedure induced by the secretion of numerous growth factors from endothelial cells. Vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (FGF2), transforming growth factor-β1, 2, 3 (TGFB1, 2, 3), and transforming growth factor-β receptors (TGFBR1, 2, 3) mRNA expression pattern was evaluated in tissue samples with cervical intraepithelial neoplasia (CIN) and cervical cancer, compared to that of normal cervical tissues, and correlated to the clinical stage of the disease. Transcript levels of the above genes were assessed by RT-PCR analysis in a total of 44 cervical specimens. VEGF, TGFB1, TGFBR1, and FGF2 transcript levels were significantly different in the normal, CIN and cancer specimen groups (
P=0.015, 0.001, 0.008, and 0.029, respectively). Higher TGFBR1 mRNA levels were observed in parallel with increased severity of the lesion, whereas FGF2 exhibited lower transcript levels. A highly significant increase of VEGF mRNA expression was found upon cervical neoplastic transformation (
P<0.0001). High-grade squamous intraepithelial lesions exhibited higher VEGF mRNA levels than low-grade lesions (
P=0.039). TGFBR1 and TGFBR3 receptors demonstrated significant co-expressions with TGFB2 (
P<0.0001), and TGFB1 (
P=0.005 and 0.002, respectively) in normal cervical specimens. However, a disruption of co-expression patterns was observed in the groups of CIN and cancer cases, compared to normal tissues. Our findings show that VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix. The involvement of the examined markers in cervical carcinogenesis is furthermore supported by the observed disruption of their mRNA co-expression patterns.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>15797633</pmid><doi>10.1016/j.canlet.2004.08.021</doi><tpages>14</tpages></addata></record> |
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| ispartof | Cancer letters, 2005-04, Vol.221 (1), p.105-118 |
| issn | 0304-3835 1872-7980 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Activin Receptors, Type I - genetics Adult Angiogenesis Cell growth Cervical cancer Cervical intraepithelial neoplasia Cervical Intraepithelial Neoplasia - genetics Cervix Uteri - metabolism Disease Female Fibroblast Growth Factor 2 - genetics Humans Middle Aged mRNA Expression Protein-Serine-Threonine Kinases Receptors, Transforming Growth Factor beta - genetics Reproductive system RNA, Messenger - metabolism Rodents RT-PCR Studies Transforming Growth Factor beta - genetics Transforming Growth Factor beta1 Transforming Growth Factor beta2 Uterine Cervical Neoplasms - genetics Vascular Endothelial Growth Factors - genetics |
| title | VEGF, FGF2, TGFB1 and TGFBR1 mRNA expression levels correlate with the malignant transformation of the uterine cervix |
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