Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing
The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expre...
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| Veröffentlicht in: | Transgenic research 1999-06, Vol.8 (3), p.203-213 |
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| description | The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated leaf disk transformation. Progeny from R0 lines was screened for resistance to TuMV-ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady-state RNA analysis and nuclear run-on assay of a line showed that the resistance was RNA-mediated through the post-transcriptional gene silencing mechanism. |
| doi_str_mv | 10.1023/A:1008915007271 |
| format | Article |
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Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated leaf disk transformation. Progeny from R0 lines was screened for resistance to TuMV-ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady-state RNA analysis and nuclear run-on assay of a line showed that the resistance was RNA-mediated through the post-transcriptional gene silencing mechanism.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1023/A:1008915007271</identifier><identifier>PMID: 10478490</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Amino Acid Sequence ; Bacteria ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Capsid - genetics ; Cloning, Molecular ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; Genes ; Genetic engineering ; Genetic technics ; Methods. Procedures. Technologies ; Molecular Sequence Data ; Nicotiana - genetics ; Nicotiana - growth & development ; Nicotiana - virology ; Nicotiana benthamiana ; Phytopathology. Animal pests. Plant and forest protection ; Plant Diseases - virology ; Plant viruses and viroids ; Plants, Genetically Modified - virology ; Plants, Toxic ; Potyvirus - genetics ; Proteins ; Sequence Analysis, DNA ; Transcription, Genetic ; Transformation, Genetic ; Transgenes ; Transgenic animals and transgenic plants ; Transgenic plants ; Turnip mosaic virus</subject><ispartof>Transgenic research, 1999-06, Vol.8 (3), p.203-213</ispartof><rights>2000 INIST-CNRS</rights><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-8de7140a1a5409e5e0f89c9ac9dccd2c7a05f52568443ef028c30eea4166fe013</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1195864$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10478490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JAN, F.-J</creatorcontrib><creatorcontrib>PANG, S.-Z</creatorcontrib><creatorcontrib>FAGOAGA, C</creatorcontrib><creatorcontrib>GONSALVES, D</creatorcontrib><title>Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><description>The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated leaf disk transformation. Progeny from R0 lines was screened for resistance to TuMV-ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady-state RNA analysis and nuclear run-on assay of a line showed that the resistance was RNA-mediated through the post-transcriptional gene silencing mechanism.</description><subject>Amino Acid Sequence</subject><subject>Bacteria</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Capsid - genetics</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - growth & development</subject><subject>Nicotiana - virology</subject><subject>Nicotiana benthamiana</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Diseases - virology</subject><subject>Plant viruses and viroids</subject><subject>Plants, Genetically Modified - virology</subject><subject>Plants, Toxic</subject><subject>Potyvirus - genetics</subject><subject>Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>Transcription, Genetic</subject><subject>Transformation, Genetic</subject><subject>Transgenes</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Turnip mosaic virus</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0EFP3DAQBWCrAsGy7bm3ykKIW-g4thOb2wpBWwnBhZ6jWWdCvUqc1HaQ9t8T2q2Qenpz-GY0eox9FnAloJRfN9cCwFihAeqyFh_YSuhaFlZW5oitwFZlYYywp-wspR3AG5Yn7FSAqo2ysGK7pzkGP_FhTOgdn8a8f_FxTjxS8iljcMR94A_ejdljQL6lkH_h8GduKfoXavl2vyymXOSIIbnop-zHgD1_pkA8-Z6C8-H5IzvusE_06ZBr9vPu9unme3H_-O3Hzea-cFJBLkxLtVCAArUCS5qgM9ZZdLZ1ri1djaA7XerKKCWpg9I4CUSoRFV1BEKu2eXfu1Mcf8-UcjP45KjvMdA4p0bUsrTKVAs8_w_uxqWN5bem1lJbUy6xZl8OaN4O1DZT9APGffOvwgVcHAAmh323dOB8enfCalMp-QrE8YFK</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>JAN, F.-J</creator><creator>PANG, S.-Z</creator><creator>FAGOAGA, C</creator><creator>GONSALVES, D</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>19990601</creationdate><title>Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing</title><author>JAN, F.-J ; PANG, S.-Z ; FAGOAGA, C ; GONSALVES, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-8de7140a1a5409e5e0f89c9ac9dccd2c7a05f52568443ef028c30eea4166fe013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Bacteria</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Capsid - genetics</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - growth & development</topic><topic>Nicotiana - virology</topic><topic>Nicotiana benthamiana</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Diseases - virology</topic><topic>Plant viruses and viroids</topic><topic>Plants, Genetically Modified - virology</topic><topic>Plants, Toxic</topic><topic>Potyvirus - genetics</topic><topic>Proteins</topic><topic>Sequence Analysis, DNA</topic><topic>Transcription, Genetic</topic><topic>Transformation, Genetic</topic><topic>Transgenes</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>Turnip mosaic virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JAN, F.-J</creatorcontrib><creatorcontrib>PANG, S.-Z</creatorcontrib><creatorcontrib>FAGOAGA, C</creatorcontrib><creatorcontrib>GONSALVES, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JAN, F.-J</au><au>PANG, S.-Z</au><au>FAGOAGA, C</au><au>GONSALVES, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing</atitle><jtitle>Transgenic research</jtitle><addtitle>Transgenic Res</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>8</volume><issue>3</issue><spage>203</spage><epage>213</epage><pages>203-213</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>The coat protein (CP) gene of turnip mosaic potyvirus isolate ESC8 (TuMV-ESC8) was cloned and sequenced. Comparisons of the 867-nucleotide (nt) CP region with those of 11 TuMV isolates showed 86.7-89.3% nucleotide identity and 92.4-95.5% amino acid identity. The CP gene was cloned into a plant expression vector and transformed into Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated leaf disk transformation. Progeny from R0 lines was screened for resistance to TuMV-ESC8. Five of 29 tested lines showed TuMV protection in more than 50% of their progeny. Interestingly, some of the resistant plants transformed with the CP gene of TuMV displayed mild mosaicism in the new growing leaves at the later stages of evaluation; but these mosaic symptoms disappeared when the leaves were fully expanded. Collective data from steady-state RNA analysis and nuclear run-on assay of a line showed that the resistance was RNA-mediated through the post-transcriptional gene silencing mechanism.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>10478490</pmid><doi>10.1023/A:1008915007271</doi><tpages>11</tpages></addata></record> |
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| subjects | Amino Acid Sequence Bacteria Base Sequence Biological and medical sciences Biotechnology Capsid - genetics Cloning, Molecular Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Genes Genetic engineering Genetic technics Methods. Procedures. Technologies Molecular Sequence Data Nicotiana - genetics Nicotiana - growth & development Nicotiana - virology Nicotiana benthamiana Phytopathology. Animal pests. Plant and forest protection Plant Diseases - virology Plant viruses and viroids Plants, Genetically Modified - virology Plants, Toxic Potyvirus - genetics Proteins Sequence Analysis, DNA Transcription, Genetic Transformation, Genetic Transgenes Transgenic animals and transgenic plants Transgenic plants Turnip mosaic virus |
| title | Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing |
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